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Development and Identification of Introgression Lines from Cross of oryza sativa and oryza minuta

Rice science 2013年第2期20卷 95-102页

作者： GUO Si-bin WEI Yu LI Xiao-qiong LIU Kai-qiang HUANG Feng-kuan CHEN Cai-hong GAO Guo-qing Rice Research Institute Guangxi Academy of Agricultural Sciences Guangxi Crop Genetic Improvement and Biotechnology Laboratory Plant Protection Research Institute Guangxi Academy of Agricultural Sciences

Introgression line population is effectively used in mapping quantitative trait loci（QTLs）,identifying favorable genes,discovering hidden genetic variation,evaluating the action or interaction of QTLs in multiple conditions and providing the favorable experimental materials for plant breeding and genetic *** this study,an advanced backcross and consecutive selfing strategy was used to develop introgression lines（ILs）,which derived from an accession of oryza minuta（accession No.101133） with BBCC genome,as the donor,and an elite indica cultivar IR24（***）,as the *** segments from *** were screened using 164 polymorphic simple sequence repeat（SSR） markers in the genome of each *** segments carried by 131 ILs covered the whole *** *** average number of homozygous *** segments per introgression line was about *** average length of introgressed segments was approximate 14.78 cM,and about 79.64% of these segments had sizes less than 20 *** the genome of each introgression line,the *** chromosomal segments harbored chromosomal fragments of *** ranging from 1.15% to 27.6%,with an overall average of 8.57%.At each locus,the ratio of substitution of *** alleles had a range of 1.5% 25.2%,with an average of 8.3%.Based on the evaluation of the phenotype of these ILs,a wide range of alterations in morphological and yield-related traits were *** inoculation,ILs 41,11 and 7 showed high resistance to bacterial blight,brown planthopper and whitebacked planthopper,*** *** ILs will serve as genetic materials for identifying and using favorable genes from ***.

关键词： oryza sativa oryza minuta introgression line bacterial blight brown planthopper whitebacked planthopper

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Identification of quantitative trait loci for resistance to white-backed planthopper using introgression lines from oryza minuta

Identification of quantitative trait loci for resistance to ...

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第十四届全国植物基因组学大会

作者： Sibin Guo Guoqing Gao Rice Research Institute Guangxi Academy of Agricultural Sciences

The whitebacked planthopper(WBPH),Sogatella furcifera Horvath,is one of the most serious sucking insect pest of rice,oryza sativa *** this study,Quantitative trait loci(QTLs) for resistance to WBPH,were determined usi... 详细信息

关键词： whitebacked planthopper quantitative trait locus introgression lines oryza minuta

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Ortholog Alleles at Xa3/Xa26 Locus Confer Conserved Race-Specific Resistance against Xanthomonas oryzae in Rice

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Molecular Plant 2012年第1期5卷 281-290页

作者： Hong-Jing Li Xiang-Hua Li Jing-Hua Xiao Rod A.Wing Shi-Ping Wang National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Arizona Genomics Institute School of Plant SciencesBIO5 InstituteUniversity of ArizonaTucsonAZ 85721USA

The rice disease resistance （R） gene Xa3/Xa26 （having also been named Xa3 and Xa26） against Xanthomonas oryzae pv. oryzae （Xoo）, which causes bacterial blight disease, belongs to a multiple gene family clustered in chromosome 11 and is from an AA genome rice cultivar （oryza sativa L.）. This family encodes leucine-rich repeat （LRR） receptor kinase- type proteins, Here, we show that the orthologs （alleles） of Xa3/Xa26, Xa3/Xa26-2, and Xa3/Xa26-3, from wild oryza spe- cies O. officinalis （CC genome） and O. minuta （BBCC genome）, respectively, were also R genes against Xoo. Xa3/Xa26-2 and Xa3/Xa26-3 conferred resistance to 16 of the 18 Xoo strains examined. Comparative sequence analysis of the Xa3/Xa26 families in the two wild oryza species showed that Xa3/Xa26-3 appeared to have originated from the CC genome of O. minuta. The predicted proteins encoded by Xa3/Xa26, Xa3/Xa26-2, and Xa3/Xa26-3 share 91-99% sequence identity and 94-99% sequence similarity. Transgenic plants carrying a single copy of Xa3/Xa26, Xa3/Xa26-2, or Xa3PXa26-3, in the same genetic background, showed a similar resistance spectrum to a set of Xoo strains, although plants carrying Xa3/Xa26-2 or Xa3/Xa26-3 showed lower resistance levels than the plants carrying Xa3/Xa26. These results suggest that the Xa3/Xa26 locus predates the speciation of A and C genome, which is approximately 7.5 million years ago. Thus, the resistance spec- ificity of this locus has been conserved for a long time.

关键词： Bacterial blight broad-spectrum resistance durable resistance oryza officinalis oryza minuta oryza sativa.

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Identification of Intergeneric Hybrid Plants Between oryza sativa and *** via GISH and RAPD

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Agricultural Sciences in China 2003年第12期2卷 1297-1301页

作者： YU Shun-wu 1, 2, CHEN Bao-tang 1, TAO Ai-lin 1 and ZHANG Duan-pin 1 ( 1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P. R. China 2Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200030, P. R. China) National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P. R. China Plant Biotechnology Research Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 P. R. China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P. R. China

To transfer desirable resistance traits from O. minuta to O. sativa, intergeneric hybrid plants between O. sativa (AA, 2n=2X=24) and O. minuta (BBCC, 2n=4X=48) were produced by embryo rescue after sexual cross. Morphological observation and chromosome counts indicated their hybrid status (ABC, 2n=3X=36). Genomic in situ hybridization (GISH) was further applied to confirm the parentage of the chromosomes of F 1 hybrids. Chromosomes of O. minuta and O. sativa were distinguishable in the hybrids in different fluorescence colors. GISH indicated that A and BC chromosomes were not randomly assembled in a cell. RAPD profiles unequivocally revealed their hybrids with double parent patterns. The results of blast tests showed that the hybrids had obtained disease resistance from O. minuta, and had a level of susceptibility between the parents.

关键词： Genomic in situ hybridization Hybrid oryza minuta oryza sativa Blast

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