检索结果-南通市图书馆

Development of an analytical method based on solid-phase extraction and LC-MS/MS for the monitoring of current-use pesticides and their metabolites in human urine

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2022年第1期34卷 153-163页

作者： Beibei Gao Giulia Poma Govindan Malarvannan Catalina Dumitrascu Michiel Bastiaensen Minghua Wang Adrian Covaci Toxicological Centre University of AntwerpWilrijk 2610Belgium Department of Pesticide Science College of Plant ProtectionState and Local Joint Engineering Research Center of Green Pesticide Invention and ApplicationNanjing Agricultural UniversityNanjing 210095China

Pyrethroids,organophosphorus pesticides and fipronil have been listed as priority chemicals in human biomonitoring studies because of their wide use and potential health effects in humans.The determination of 13 pesticides,including pyrethroids(deltamethrin,cypermethrin,permethrin,cyfluthrin,bifenthrin),organophosphorus(chlorpyrifos,chlorpyrifosmethyl,and malathion),fipronil,neonicotinoids(imidacloprid,acetamiprid and thiacloprid)and triazole(prothioconazole),together with 13 corresponding metabolites in human urine samples was achieved by solid-phase extraction and analysis by liquid chromatography coupled to tandem mass spectrometry.All targeted compounds,except malathion dicarboxylic acid,were measured with a mean within-accuracy(n=5)of 71%–114%(RSD:1%–14%)and between-run(n=15)accuracy of 80%–118%(RSD:2%–14%).Limits of quantitation of the targeted analytes ranged from 0.1 to 16 pg/m L.The detection result of urine samples from 25 volunteers indicated that the detection frequencies of 3,5,6-trichloro-2-pyridinol(median:448 pg/m L),6-chloropyridine-3-carboxylic acid(median:193 pg/m L),2-methyl-3-phenylbenzoic acid(median:181 pg/m L),3-phenoxybenzoic acid(median:99 pg/m L),2-isopropyl-6-methyl-4-pyrimidinol(median:77 pg/m L),cyfluthrin(median:59 pg/m L),cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid(cis-DCCA,median:53 pg/m L),trans-DCCA(median:25 pg/m L),prothioconazole(median:21 pg/m L),imidacloprid(median:7 pg/m L),and prothioconazole-desthio(median:1 pg/m L)were>50%.The obtained results show that the validated method is suitable for the human biomonitoring of these currentuse pesticides and their metabolites.

关键词： Pyrethroids organophosphorus Fipronil Neonicotinoids Urine Biomonitoring

Role of penehyclidine in acute organophosphorus pesticide poisoning

在线全文

学校读者我要写书评

暂无评论

World Journal of Emergency Medicine 2020年第1期11卷 37-47页

作者： Shi-yuan Yu Yan-xia Gao Joseph Walline Xin Lu Li-na Zhao Yuan-xu Huang Jiang Tao An-yong Yu Na Ta Ren-ju Xiao Yi Li Department of Emergency Medicine Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijingChina Department of Emergency Medicine the First Affi liated Hospital of Zhengzhou UniversityZhengzhouChina Accident and Emergency Medicine Academic Unit Prince of Wales Hospitalthe Chinese University of Hong KongHong KongChina Department of Emergency Medicine the Fourth Affi liated Hospital of Jishou UniversityHuaihuaChina Department of Emergency Medicine the First Affi liated Hospital of Zunyi Medical UniversityZunyiChina Department of Emergency Medicine Chifeng Municipal HospitalChifengChina Department of Emergency Medicine Xingyi People's HospitalXingyiChina

BACKGROUND:Penehyclidine is a newly developed anticholinergic agent.We aimed to investigate the role of penehyclidine in acute organophosphorus pesticide poisoning(OP)patients.METHODS:We searched the Pubmed,Cochrane library,EMBASE,Chinese National Knowledge Infrastructure(CNKI),Chinese Biomedical literature(CBM)and Wanfang databases.Randomized controlled trials(RCTs)recruiting acute OP patients were identifi ed for meta-analysis.Main outcomes included cure rate,mortality rate,time to atropinization,time to 60%normal acetylcholinesterase(AchE)level,rate of intermediate syndrome(IMS)and rate of adverse drug reactions(ADR).RESULTS:Sixteen RCTs involving 1,334 patients were identifi ed.Compared with the atropineor penehyclidine-alone groups,atropine combined with penehyclidine significantly increased the cure rate(penehyclidine+atropine vs.atropine,0.97 vs.0.86,RR 1.13,95%CI[1.07–1.19];penehyclidine+atropine vs.penehyclidine,0.93 vs.0.80,RR 1.08,95%CI[1.01–1.15])and reduced the mortality rate(penehyclidine+atropine vs.atropine,0.015 vs.0.11,RR 0.17,95%CI[0.06–0.49];penehyclidine+atropine vs.penehyclidine,0.13 vs.0.08,RR 0.23,95%CI[0.04–1.28]).Atropine combined with penehyclidine in OP patients also helped reduce the time to atropinization and AchE recovery,the rate of IMS and the rate of ADR.Compared with a single dose of atropine,a single dose of penehyclidine also signifi cantly elevated the cure rate,reduced times to atropinization,AchE recovery,and rate of IMS.CONCLUSION:Atropine combined with penehyclidine benefi ts OP patients by enhancing the cure rate,mortality rate,time to atropinization,AchE recovery,IMS rate,total ADR and duration of hospitalization.Penehyclidine combined with atropine is likely a better initial therapy for OP patients than atropine alone.

关键词： Penehyclidine organophosphorus pesticide poisoning Meta-analysis

Biochar-mediated regulation of greenhouse gas emission and toxicity reduction in bioremediation of organophosphorus pesticide-contaminated soils

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2018年第12期26卷 2592-2600页

作者： Meinan Zhen Benru Song Xiaomei Liu Radhika Chandankere Jingchun Tang Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education) Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation College of Environmental Science and Engineering Nankai University Department of Building Civil and Environmental Engineering Concordia University

organophosphorus pesticides(OPPs) are a set of toxic persistent organic pollutants(POPs) present in the environment. Recently, biochar-mediated bioremediation has exhibited many advantages over conventional methods for the remediation of pesticide-contaminated soil. In the present study, biochar and nitrogen fertilizer(NH_4NO_3)were employed to remediate OPP-contaminated soil and the greenhouse gas(GHG) emission during 90 days of incubation was investigated. After thermal desorption treatment, the content of organophosphorus pesticides reduced from 175.61 μg·kg^(-1) to 62.68 μg·kg^(-1). The addition of NH_4NO_3 in the following bioremediation led to larger reduction(34.35%) of the pesticide concentration than that of biochar(31.90%) for the contaminated soils with thermal desorption treatment, while the simultaneous addition of biochar and NH_4NO_3 led to the largest reduction of pesticide concentration(11.07%) for the soil without thermal desorption treatment. The addition of biochar and NH_4NO_3 only slightly increased the emission rate of GHGs from the soil without thermal treatment,but remarkably increased the emission rate of GHGs from the soil after thermal treatment. In most cases, the addition of NH_4NO_3 is more effective than biochar to promote the degradation of pesticide, but also exhibited higher GHG emission. The microbial community analysis suggests that the enhanced degradation of pesticide is mainly owing to the increased activity of microorganism.

关键词： organophosphorus pesticides Biochar NH3NO4 Remediation Greenhouse gas Microbial community

Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies:Characterization and stability studies

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2019年第1期27卷 191-199页

作者： Meisam Sharifi Seyed-Mortaza Robatjazi Minoo Sadri Jafar Mohammadian Mosaabadi Department of Bioscience and Biotechnology Malek Ashtar University of Technology

The plant cellulose powder was activated by two different methods using 1,4-butanediol diglycidyl ether(BTDE)and 1,1′-Carbonyldiimidazole(CDI) as the chemical coupling agents.organophosphorus hydrolase(OPH) from Flavobacterium ATCC 27551 was immobilized on any of activated support through covalent bonding.The optimal conditions of affecting parameters on enzyme immobilization in both methods were found, and it was demonstrated that the highest activity yields of immobilized OPH onto epoxy and CDI treated cellulose were 68.32%and 73.51%, respectively.The surface treatment of cellulose via covalent coupling with BTDE and CDI agents was proved by FTIR analysis.The kinetic constants of the free and immobilized enzymes were determined, and it was showed that both immobilization techniques moderately increased the Kmvalue of the free OPH.The improvements in storage and thermal stability were investigated and depicted that the half-life of immobilized OPH over the surface of epoxy modified cellulose had a better growth compared to the free and immobilized enzymes onto CDI treated support.Also, the pH stability of the immobilized preparations was enhanced relative to the free counterpart and revealed that all enzyme samples would have the same optimum pH value for stability at 9.0.Additionally, the immobilized OPH onto epoxy and CDI activated cellulose retained about 59% and 68% of their initial activity after ten turns of batch operation, respectively.The results demonstrated the high performance of OPH enzyme in immobilized state onto an inexpensive support with the potential of industrial applications.

关键词： organophosphorus hydrolase Cellulose powder Activation Immobilization Stability

Complete genome sequence of Bacillus amyloliquefaciens YP6, a plant growth rhizobacterium efficiently degrading a wide range of organophosphorus pesticides

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2019年第11期18卷 2668-2672页

作者： MENG Di ZHAI Li-xin TIAN Qiao-peng GUAN Zheng-bing CAI Yu-jie LIAO Xiang-ru Key Laboratory of Industrial Biotechnology Ministry of Education School of Biotechnology Jiangnan University

Bacillus amyloliquefaciens YP6,a plant growth promoting rhizobacteria,is capable of efficiently degrading a wide range of organophosphorus pesticides(OPs).Here,we report the complete genome sequence of this bacterium with a genome size of 4009619 bp,4210 protein-coding genes and an average GC content of 45.9%.Based on the genome sequence,several genes previously described as being involved in solubilizing-phosphorus,OPs-degradation,indole-3-acetic acid(IAA)and siderophores synthesis.Interestingly,compared with the genomes of B.amyloliquefaciens species,strain YP6 had larger genome size and the most protein-coding genes.Moreover,the four categories of“cell envelope biogenesis,outer membrane(M),”“translation,ribosomal structure and biogenesis(J),”“transcription(K),”and“signal transduction mechanisms(T)”were fewer.These differences may be related to extensive environmental adaptability of the genus B.amyloliquefaciens.These results expand the application potential of strain YP6 for environmental bioremediation,provide gene resources involved in OPs degradation for biotechnology and gene engineering,and contribute to provide insights into the relationship between microorganism and living environment.

关键词： organophosphorus pesticides genome sequence plant growth promoting rhizobacteria Bacillus amyloliquefaciens

Study on the Quantitative Relationship between the Structure and Toxicity of organophosphorus Pesticide

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2015年第10期34卷 1473-1478页

作者：莫凌云覃如琼覃礼堂曾鸿鹄梁延鹏 Department of Environmental Science and Engineering Guilin University of Technology Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology Guangxi Polytechnic of Construction

The molecular electronegativity distance vector（MEDV） was applied to characterize the molecular structures of 30 organophosphorous compounds. Optimum MEDV descriptors were selected by using the variable selection and modeling method based on the prediction（VSMP） technique. The quantitative structure-toxicity relationship（QSTR） model was built for acute toxicity（96h pLC50） of organophosphorous compounds to steelhead. The developed QSTR model with strictly internal and external validations presents relatively high correlation coefficient（R2） of 0.9518, leave-one-out（LOO） cross-validated correlation coefficient（Q2LOO） of 0.9355, and leave-many-out（LMO） cross-validated correlation coefficient（Q2LMO） of 0.9290. The robustness of the model was confirmed by the y-randomization test（R2yrand = 0.0772 and Q2 yrand = –0.5313） and bootstrapping（R2bstr = 0.9502 and Q2 bstr = 0.9177） method. The result of external validation, Q2F1 = 0.9336, Q2F2 = 0.9336, Q2F3 = 0.9447, r2 m = 0.8120, and CCC = 0.9602, shows that the QSTR model has a high predictive ability.

关键词： molecular electronegativity distance vector（MEDV） QSTR organophosphorus pLC50

The Chemical Kinetics in Migration of organophosphorus in Clay with Different pH

在线全文

学校读者我要写书评

暂无评论

Acta Geologica Sinica(English Edition) 2013年第4期87卷 1162-1167页

作者： LI Hongyan ZHANG Guangxin SONG Dingfeng WANG Yanliang LI Jianping LI Xuqian Key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Jilin Institute of Geo-Environment Monitoring SIPPR Engineering Group Co. Ltd Limited Company of Metallurgy Engineering of China College of Environment and Resources Jilin University

In order to study the characters of chemical kinetics for organophosphorus migration in clay with different pH, waste of organophosphorus was put under pressure to leakage permeating the cohesive soil, and simulate the process of organophosphorus leakage permeating the Aquitard, searching the characters of chemical kinetics for organophosphorus migration in clay with different pH. It is shown that the ability of migration of organophosphorus leakage permeating the cohesive soil fall with increase of pH; the penetration rate of organophosphorus is about 1.25% when pH is 7.5, organophosphorus has not penetrated the cohesive soil when pH is equal or greater than 8.5. The effect of retardarce is obvious. Concentration of PO43？ that comes from the mineralization of organophosphorus is lowered slightly with increases of pH of clay, and rise with extension of time. The Chemical Kinetics equation is log c=-0.1pH＋0.2172k1t＋S.

关键词： organophosphorus pH dynamics of migration

Mn-mediated reductive C(sp3)–Si coupling of activated secondary alkyl bromides with chlorosilanes

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第12期33卷 5061-5064页

作者： Liangliang Qi Xiaobo Pang Kai Yin Qiu-Quan Pan Xiao-Xue Wei Xing-Zhong Shu State Key Laboratory of Applied Organic Chemistry(SKLAOC) College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China School of Chemistry and Chemical Engineering Southeast UniversityJiangsu Optoelectronic Functional Materials and Engineering LaboratoryNanjing 211189China Zhejiang Nanjiao Chemistry Co. Ltd.Shangyu Economic and Technological Development ZoneShangyu 312369China

The construction of secondary alkylsilanes is a challenging subject in the synthetic community.The cross-coupling provides a practical solution to address this problem,but it typically relies on organometallic species.Herein,we report an Mn-mediated reductive C(sp^(3))-Si coupling to synthesize these compounds from alkyl and silyl electrophiles.This approach avoids the requirement for activation of Si-Cl by transition metals and thus allows for the coupling of various common chlorosilanes.The reaction proceeds under mild conditions and shows good functional group compatibility.The method offers access toα-silylated organophosphorus and sulfones with a scope that is complementary to those obtained from the established methods.

关键词： Organosilanes Alkylsilanes Reductive coupling Chlorosilanes organophosphorus Sulfones

Studies　on　organophosphorus　Compounds　97．Cu_2Ocatalyzed　Reaction　of　Isocyanoacetate　with　1－Oxoalkylphosphonate．A　Novel　Stereoselective　Synthesis　of　β－Phosphoryl　α，β－Dehydroamino　Acid　Derivatives

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1996年第9期7卷 797-798页

作者： Wei Sheng HUANG Yi adn ZHANG and Cheng Ye YUAN(Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences.354 Fenglin Lu.Shanghai 200032) Shanghai Inst. of Organic Chemistry Chinese Academy of Sciences 354 Fenglin Lu Shanghai 200032

Cu2O-Catalyzed reaction of isocyanoacetate with 1-oxoalkylphosphonates afford predominantly Z-α-formylamino-β-phosphoryl-α,β-alkenoate.

关键词：梗梗 Reaction Novel Stereoselective Acid Derivatives Compounds organophosphorus Synthesis Dehydroamino 桑桑 Cu2Ocatalyzed