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Plant peroxisomes:Small organelles with versatility and complexity

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Journal of Integrative Plant Biology 2019年第7期61卷 782-783页

作者： Jianping Hu MSU-DOE Plant Research Laboratory and Plant Biology Department Michigan State University

Peroxisomes are small,highly dynamic,and multifunctional organelles in *** to plant survival,peroxisomes house various crucial metabolic activities,such as degradation of hydrogen peroxide(H2O2),lipid metabolism,photorespiration,and hormone biosynthesis and catabolism,and remodel their proteome in response to developmental and environmental changes(Hu et al.2012;Pan and Hu 2018).The four reviews and three research articles in this special issue on plant peroxisomes provide new insights into the diverse roles and dynamics of these structurally simple but functionally complicated organelles,raising exciting new questions for future investigations.

关键词： Plant peroxisomes Small organelles versatility complexity

Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1754-1767页

作者： Chunhua Xing Shun Chen Yue Wang Zhennan Pan Yuanjing Zou Shaochen Sun Zili Ren Yu Zhang College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China

Background:Recently,defects in mammalian oocytes maturation induced by environmental pollution results in the decreasing animal *** exposed to glyphosate is largely unavoidable because glyphosate is one of the most widely used herbicide worldwide due to its high-efficiency and broad-spectrum effects,which causes glyphosate an environmental contaminant found in soil,water and *** the last few years,the growing and wider use of glyphosate has raised great concerns about its effects of reproductive *** this study,using porcine models,we investigated effects of glyphosate on organelle functions during oocyte ***:The results showed glyphosate exposure disrupted porcine oocyte *** levels of cumulus expansion-related genes were interfered,further indicating the meiotic *** damaging effects were mediated by destruction of mitochondrial distribution and functions,which induced ROS accumulation and oxidative stress,also indicated by the decreased mRNA expression of related antioxidant enzyme *** also found an interference of endoplasmic reticulum(ER)distribution,disturbance of Ca^(2+)homeostasis,as well as fluctuation of ER stress,showing with the reduced ER stress-related mRNA or protein expression,which could indicate the dysfunction of ER for protein processing and signal transduction in glyphosate-exposed ***,glyphosate exposure induced the disruption of lysosome function for autophagy,showing with the decrease of LAMP2 expression and autophagy-related genes mRNA ***,our data showed the distribution of Golgi apparatus and the functions of ribosome were disturbed after glyphosate exposure,which might affect protein synthesis and ***:Collectively,our study showed that exposed to glyphosate could affect animal reproduction by compromising the quality of oocytes through its wide toxic effects on organelle functions.

关键词： Endoplasmic reticulum Glyphosate Lysosome Mitochondria organelles Oocyte maturation

The Expansion and Diversification of Pentatricopeptide Repeat RNA-Editing Factors in Plants

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Molecular Plant 2020年第2期13卷 215-230页

作者： Bernard Gutmann Santana Royan Mareike Schallenberg-Rudinger Henning Lenz Ian R.Castleden Rose McDowell Michael AVacher Julian Tonti-Filippini Charles SBond Volker Knoop Ian D.Small Australian Research Council Centre of Excellence in Plant Energy Biology The University of Western AustraliaCrawleyPerth 6009WAAustralia School of Molecular Sciences The University of Western AustraliaCrawleyPerth 6009WAAustralia IZMB-Institut fur Zellulare und Molekulare Botanik Abteilung Molekulare EvolutionUniversitat BonnKirschallee 153115 BonnGermany Present address:EditForce Inc. West zone#429Kyushu University744 MotookaNishi-KuFukuokaJapan Present address:IBG-2:Plant Sciences Forschungszentrum Jiilich GmbH52425 JulichGermany Present address:Australian eHealth Research Centre Commonwealth Scientific and Industrial Research OrganisationFloreatPerth 6014WAAustralia

The RNA-binding pentatricopeptide repeat(PPR)family comprises hundreds to thousands of genes in most plants,but only a few dozen in algae,indicating massive gene expansions during land plant *** nature and timing of these expansions has not been well defined due to the sparse sequence data available from early-diverging land plant *** this study,we exploit the comprehensive OneKP datasets of over 1000 transcriptomes from diverse plants and algae toward establishing a clear picture of the evolution of this massive gene family,focusing on the proteins typically associated with RNA editing,which show the most spectacular variation in numbers and domain composition across the plant *** characterize over 2250000 PPR motifs in over 400000 *** lycophytes,polypod ferns,and hornworts,nearly 10%of expressed protein-coding genes encode putative PPR editing factors,whereas they are absent from algae and complex-thalloid *** show that rather than a single expansion,most land plant lineages with high numbers of editing factors have continued to generate novel sequence *** identify sequence variations that imply functional differences between PPR proteins in seed plants versus non-seed plants and variations we propose to be linked to seed-plant-specific editing ***,using the sequence variations across the datasets,we develop a structural model of the catalytic DYW domain associated with C-to-U editing and identify a clade of unique DYW variants that are strong candidates as U-to-C RNA-editing factors,given their phylogenetic distribution and sequence characteristics.

关键词： RNA editing RNA-binding protein organelles

Proximity labeling: an emerging tool for probing in planta molecular interactions

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Plant Communications 2021年第2期2卷 1-15页

作者： Xinxin Yang Zhiyan Wen Dingliang Zhang Zhen Li Dawei Li Ugrappa Nagalakshmi Savithramma P.Dinesh-Kumar Yongliang Zhang State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology College of Biological SciencesChina Agricultural UniversityBeijing 100193P.R.China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Department of Plant Biology and the Genome Center College of Biological SciencesUniversity of CaliforniaDavisDavisCA 95616USA

Protein–protein interaction(PPI)networks are key to nearly all aspects of cellular ***,the identification of PPIs is important for understanding a specific biological process in an *** with conventional methods for probing PPIs,the recently described proximity labeling(PL)approach combined with mass spectrometry(MS)-based quantitative proteomics hasemerged as apowerful approach for characterizing ***,the application of PL in planta remains in its ***,we summarize recent progress in PL and its potential utilization in plant *** specifically summarize advances in PL,including the development and comparison of different PL enzymes and the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems.

关键词： protein interactions proximity labeling biotin ligase plant membrane contact sites organelles

Phosphatidylglycerol-containing ER-transport vesicles built and restore outer mitochondrial membrane and deliver nuclear DNA translation products to generate cardiolipin in the inner mitochondrial membrane

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Advances in Biological Chemistry 2012年第2期2卷 132-145页

作者： Amalia Slomiany Bronislaw L. Slomiany Research Center University of Medicine and Dentistry of New Jersey Newark USA

Phosphatidylglycerol (PG) an important membrane phospholipid required for the synthesis of diphos-phatidylglycerol (DPG) commonly known as cardiolipin (CL) was identified in the fraction of endo-plasmic reticulum (ER)-derived transport vesicles which had no affinity for Golgi. The vesicles were produced in the presence of Brefeldin A (BFA), the agent known to inhibit ER-Golgi transport, and found to display affinity to mitochondria. The analysis revealed that their cargo was not containing proteins that are transported to Golgi, and that their membrane was free of phosphatidylinositol (PI) and ceramides (Cer). The incubation of PG-containing transport vesicles with mitochondria afforded incorporation of their membrane into the Outer Mito-chondrial Membrane (OMM) and formation of lyso-phosphatidylglycerol (LPG). In turn, upon further incubation with fresh transport active cytosol, the mitochondrial LPG was converted to PG. The results of analysis of the OMM, Inner Mitochondrial Mem-brane (IMM) and Inner Mitochondrial Space Components (IMSC) strongly suggest that PG-containing transport vesicles deliver nuclear DNA translation products to the IMSC and thus facilitate CL synthesis in the IMM. In summary, our studies provide evidence that ER-generated PG-enriched transport vesicles represent the general pathway for restitution of mitochondrial membranes and the delivery of nuclear DNA translation products that generate CL, and thus sustain the mitochondrial matrix CL-dependent metabolic reactions.

关键词： ER-Transport Vesicles Mitochondrial Membranes Biogenesis Transport of Nuclear DNA Translation Products Cardiolipin Synthase Cell organelles Repair

Lipid-Protein Microinclusions in the Morphological Structures of Organelle Membranes Studied by Fluorescent Confocal Microscopy

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Advances in Biological Chemistry 2017年第1期7卷 42-59页

作者： Michael Yu. Chernyshov Vadim N. Nurminsky Natalia V. Ozolina Presidium of Irkutsk Scientific Center Siberian Branch Russian Academy of Sciences Irkutsk Russia Siberian Institute of Plant Physiology and Biochemistry Siberian Branch Russian Academy of Sciences Irkutsk Russia

Peculiar properties of morphological structures of organelle membranes were studied by fluorescent confocal microscopy. The list of objects in our experiments was represented by mitochondria, chloroplasts and vacuoles. During this study, identification of lipid microinclusions having the form of such lipid-protein structural microformations as lipid-protein microdomains, vesicles and membrane tubular structures (cytoplasmic transvacuolar strands and nanotubes) located in organelle membranes or bound up with them was conducted. Such membrane probes as laurdan, DPH, ANS and bis-ANS were used. Comparison of fluorescence intensity of these membrane probes was conducted. This investigation of the morphological properties of lipid-protein structural microformations was accompanied with analysis of 1) the phase state and 2) dynamics of microviscosity variations in the membrane elements of isolated plant cell organelles. Distributions of laurdan fluorescence generalized polarization (GP) values for the membrane on the whole and for the intensively fluorescing membrane segments were obtained. It was discovered that the microviscosity of intensively fluorescing membrane segments essentially differed from the microviscosity of the rest part of the membrane. In conclusion, some results of the study of peculiar properties of lipid-protein structural microformations related to the structure of organelle membranes and the discoveries made in this investigation are discussed.

关键词： Identification of Lipids Morphological Structure of Cell organelles Morphological Structure of Vacuolar Membrane Fluorescent Confocal Microscopy Fluorescent Probes Lipid-Protein Structural Membrane Microinclusions Types of Microformations Identified Lipid-Protein Microdomains Lipid-Protein Membrane Nanotubes Vesicles Cytoplasmic Strands Microviscosity