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3D micro-devices for enhancing the lateral resolution in optical microscopy

Light(Advanced Manufacturing) 2024年第2期5卷 50-63页

作者： Gordon Zyla Goran Maconi Anton Nolvi Jan Marx Dimitra Ladika Ari Salmi Vasileia Melissinaki Ivan Kassamakov Maria Farsari Institute of Electronic Structure and Laser Foundation for Research and Technology-HellasHeraklionGreece Electronics Research Laboratory Department of PhysicsUniversity of HelsinkiHelsinkiFinland Applied Laser Technologies Ruhr University BochumBochumGermany

Although optical microscopy is a widely used technique across various multidisciplinary fields for inspecting small-scale objects,surfaces or organisms,it faces a significant limitation:the lateral resolution of optical microscopes is fundamentally constrained by light *** micro-spheres,however,offer a promising solution to this issue as they are capable of significantly enhancing lateral resolution through extraordinary phenomena,such as a photonic *** upon the potential of dielectric micro-spheres,this paper introduces a novel approach for fabricating 3D micro-devices designed to enhance lateral resolution in optical *** proposed 3D micro-device comprises a modified coverslip and a micro-sphere,facilitating easy handling and integration into any existing optical *** manufacture the device,two advanced femtosecond laser techniques are employed:femtosecond laser ablation and multi-photon *** laser ablation was employed to create a micro-hole in the coverslip,which allows light to be focused through this ***-photon lithography was used to fabricate a micro-sphere with a diameter of 20μm,along with a cantilever that positions the above the processed micro-hole and connect it with the *** this context,advanced processing strategies for multi-photon lithography to produce a micro-sphere with superior surface roughness and almost perfect geometry(λ/8)from a Zr-based hybrid photoresist are *** performance of the micro-device was evaluated using Mirau-type coherence scanning interferometry in conjunction with white light illumination at a central wavelength of 600 nm and a calibration grid(Λ=0.28μm,h>50 nm).Here,the 3D micro-device proved to be capable of enhancing lateral resolution beyond the limits achievable with conventional lenses or microscope objectives when used in ***,it maintained the high axial resolution characteristic of Mirau-type coherenc

关键词： Photonic nanojet Multi-photon lithography optical microscopy Micro-optics Multi-photon grayscale lithography Extreme manufacturing

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Observation of the in—vivo Reporter of Green Fluorescent Protein in a Plant Root by Scanning Near—Field optical microscopy

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Chinese Physics Letters 2002年第9期19卷 1389-1391页

作者：孙家林刘进元等 KeyLaboratoryofAtomicandMolecularNanosciencesofEducationMinistryandDepartmentofPhysics TsinghuaUniversityBeijing100084 DepartmentofLifeScienceandBiotechnology TsinghuaUniversity

An in vivo reporter of green fluorescent protein (GFP) in a living plant root has been imaged by scanning near-field optical microscopy (SNOM) in the transmission mode. The exciting light is 488 nm wavelength of the argon ion laser and the bandpass filter (514±10) nm is put into the detecting optical pathway. The results indicate that, in the living plant cells, the GFPs gather together and form an area of 2-4 μm, rather than being individually distributed. The transmission coefficient of the eigenfunction is incorporated into Bethe's theoretical model modified by Grober, and the near-field excited light intensity is calculated along the fibre probe axis (z-axis) in the air medium and biological medium. Based on this, along the z-axis direction of the GFP detected in the sample, numerous GFPs locate near the epidermal cells wall (in the range of 0-38 nm) in the living root. The experiments show that SNOM has an advantage of optical nanometre-scale resolution along the z-axis

关键词： Root Argon Ion Lasers modes of transmission Near field scanning optical microscopy Eigenfunctions near-field optical microscopy Green Fluorescent Proteins

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When optical microscopy meets all-optical analog computing:A brief review

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Frontiers of physics 2023年第4期18卷 249-264页

作者： Yichang Shou Jiawei Liu Hailu Luo Laboratory for Spin Photonics School of Physics and ElectronicsHunan UniversityChangsha 410082China

As a revolutionary observation tool in life science,biomedical,and material science,optical microscopy allows imaging of samples with high spatial resolution and a wide field of ***,conventional microscopy methods are limited to single imaging and cannot accomplish real-time image *** edge detection,image enhancement and phase visualization schemes have attracted great interest with the rapid development of optical analog *** two main physical mechanisms that enable optical analog computing originate from two geometric phases:the spin-redirection Rytov-Vlasimirskii-Berry(RVB)phase and the Pancharatnam-Berry(PB)***,we review the basic principles and recent research progress of the RVB phase and PB phase based optical *** we focus on the innovative and emerging applications of optical analog computing in microscopic *** analog computing is accelerating the transformation of information processing from classical imaging to quantum *** intersection with optical microscopy opens opportunities for the development of versatile and compact optical microscopy systems.

关键词： optical microscopy optical analog computing all-optical image processing

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Missed Opportunities for Diagnosing Bacilliferous Pulmonary Tuberculosis by optical microscopy versus GeneXpert MTB/RIF in Endemic Areas

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Advances in Infectious Diseases 2023年第4期13卷 652-659页

作者： Aba Yapo Thomas Nassoué Dobré Olivia Yéo Liomehin Monemo Pacome Yapo Matine Tatiana Achi Hoboueu Vincent Department of Infectious and Tropical Diseases University Hospital of Bouake Bouake Ivory Coast University Alassane Ouattara of Bouake Bouake Ivory Coast Department of Pneumology University Hospital of Bouake Bouake Ivory Coast Department of Bacteriology & Virology University Hospital of Bouake Bouake Ivory Coast

Objective: To assess the missed opportunities from the diagnosis of bacilliferous pulmonary tuberculosis by optical microscopy compared to GeneXpert MTB/RIF between 2015 and 2019. Methods: This is a retrospective analysis of the diagnostic results of bacilliferous pulmonary tuberculosis in patients suspected of pulmonary tuberculosis at their first episode during the period. GeneXpert MTB/RIF (GeneXpert) and optical microscopy (OM) after Ziehl-Neelsen stained smear were performed on each patient’s sputum or gastric tubing fluid sample. Results: Among 341 patients suspected of pulmonary tuberculosis, 229 patients were declared bacilliferous tuberculosis by the two tests (67%), 220 patients by GeneXpert and 95 patients by OM, i.e. 64.5% versus 28% (p i.e. 58.5% of the positive cases detected by the two tests (134/229 patients) and 39.3% of the patients suspected of tuberculosis (134/341 patients). On the other hand, among 95 patients declared positive by OM, the GeneXpert ignored 9 (9.5%), i.e. 4% of all the positive cases detected by the two diagnostic tests (9/229 patients) and 3% of the patients suspected of tuberculosis (9/341 patients). The differences observed between the results of the two tests were statistically significant at the 5% threshold (p Conclusion: This study reveals missed diagnostic opportunities for bacilliferous pulmonary mycobacteriosis, statistically significant with optical microscopy than GeneXpert. The GeneXpert/optical microscopy couple could be a good contribution to the strategies for the elimination of pulmonary tuberculosis in sub-Saharan Africa.

关键词： Bacilliferous Pulmonary Tuberculosis Missed Opportunity GeneXpert MTB/RIF optical microscopy

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Multi-prior physics-enhanced neural network enables pixel super-resolution and twin-imagefree phase retrieval from single-shot hologram

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Opto-Electronic Advances 2024年第9期7卷 22-38页

作者： Xuan Tian Runze Li Tong Peng Yuge Xue Junwei Min Xing Li Chen Bai Baoli Yao State Key Laboratory of Transient Optics and Photonics Xi’an Institute of Optics and Precision MechanicsChinese Academy of SciencesXi’an 710119China University of Chinese Academy of Sciences Beijing 100049China.

Digital in-line holographic microscopy(DIHM)is a widely used interference technique for real-time reconstruction of living cells’morphological information with large space-bandwidth product and compact ***,the need for a larger pixel size of detector to improve imaging photosensitivity,field-of-view,and signal-to-noise ratio often leads to the loss of sub-pixel information and limited pixel ***,the twin-image appearing in the reconstruction severely degrades the quality of the reconstructed *** deep learning(DL)approach has emerged as a powerful tool for phase retrieval in DIHM,effectively addressing these ***,most DL-based strategies are datadriven or end-to-end net approaches,suffering from excessive data dependency and limited generalization ***,a novel multi-prior physics-enhanced neural network with pixel super-resolution(MPPN-PSR)for phase retrieval of DIHM is *** encapsulates the physical model prior,sparsity prior and deep image prior in an untrained deep neural *** effectiveness and feasibility of MPPN-PSR are demonstrated by comparing it with other traditional and learning-based phase retrieval *** the capabilities of pixel super-resolution,twin-image elimination and high-throughput jointly from a single-shot intensity measurement,the proposed DIHM approach is expected to be widely adopted in biomedical workflow and industrial measurement.

关键词： optical microscopy quantitative phase imaging digital holographic microscopy deep learning super-resolution

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A microvascular image analysis method for optical-resolution photoacoustic microscopy

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Journal of Innovative optical Health Sciences 2020年第4期3卷 69-79页

作者： Jingxiu Zhao Qian Zhao Riqiang Lin Jing Meng School of Information Science and Engineering Qufu Normal University 80 Yantai Road North Rizhao 276826P.R.China Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Institute of Biomedical and Health Engineering 1068 Xueyuan Avenue Shenzhen 518055 P.R.China

optical-resolution photoacoustic microscopy(OR-PAM)has been shown to be an excellent tool for high-resolution imaging of microvasculature,and quantitative analysis of the microvascula-ture can provide valuable information for the early diagnosis and treatment of various vascular-related *** order to address the characteristics of weak signals,discontinuity and small diameters in photoacoustic microvascular images,we propose a method adaptive to the micro-vascular segmentation in photoacoustic images,including Hessian matrix enhancement and the morphological connection *** accuracy of our vascular segmentation method is quantitatively evaluated by the multiple *** obtain more precise and continuous mi-crovascular skeletons,an improved skeleton extraction framework based on the multistencil fast marching(MSFM)method is *** carried out in vivo OR-PAM microvascular imaging in mouse ears and subcutaneous hepatoma tumor model to verify the correctness and superiority of our proposed *** with the previous methods,our proposed method can extract the microvascular network more completely,continuously and accurately,and provide an ef-fective solution for the quantitative analysis of photoacoustic microvascular images with many small branches.

关键词： Biomedical photonics photoacoustic imaging optical microscopy microvascularnetwork

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Development of Morus alba Reinforced Poly-Lactic Acid with Elevated Mechanical and Thermal Properties

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Materials Sciences and Applications 2024年第7期15卷 186-200页

作者： Girish Kumar Reddy Madda Jens Schuster Yousuf Pasha Shaik Department of Applied Logistics and Polymer Sciences University of Applied Sciences Kaiserslautern Institute for Plastics Engineering West Pfalz (IKW) Pirmasens Germany

This research investigates the mechanical and thermal properties of Morus alba combined with polylactic acid in comparison with other natural fibers. The study uses three different fiber and PLA compositions - 20%, 30%, and 40% respectively - to produce composite materials. In addition, another composite with the same fiber volume is treated with a 4% NaOH solution to improve mechanical properties. The composites are processed by twin-screw extrusion, granulation, and injection molding. Tensile strength measurements of raw fibers and NaOH-treated fibers were carried out using a single-fiber tensile test with a gauge length of 40 mm. It was observed that the NaOH surface treatment increases the resistance against tensile loading and exhibited improved properties for raw fiber strands. The diameter of the fibers was measured using optical microscopy. During this research, flexural tests, impact tests, differential scanning calorimetry (DSC), and heat deflection temperature measurements (HDT) were conducted to evaluate the mechanical and thermal properties of the developed composite samples. The results indicate that the mechanical properties of NaOH-treated Morus alba-reinforced polylactic acid outperform both virgin PLA samples and untreated Morus alba samples.

关键词： Morus Alba Fiber (MAF) White Mulberry Polylactic Acid (PLA) Sodium Hydroxide (NaOH) Solution Single Fiber Tensile Test Thermal Stability optical microscopy

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Substrate mediated dissolution of redox active nanoparticles;electron transfer over long distances

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Nano Research 2022年第1期15卷 429-437页

作者： Ruo-Chen Xie Christopher Batchelor-McAuley Minjun Yang Richard G.Compton Department of Chemistry Physical and Theoretical Chemistry LaboratoryUniversity of OxfordSouth Parks RoadOxford OXl 3QZUK

Reflective dark field microscopy is used to observe the decrease in the light scattered from Ag nanoparticles immobilised on differing solid *** nanoparticles are exposed to solutions containing halide ions,both at open circuit and under potentiostatic control,leading to the loss of the *** coupling optical and electrochemical techniques the physical origin of this transformation is demonstrated to be the electrochemical dissolution of the metal nanoparticles driven by electron transfer to ultra-trace dissolved *** dissolution kinetics of the surface-supported metal nanoparticles is compared on four substrate materials(i.e.,glass,indium titanium oxide,glassy carbon and platinum)with different electrical *** three conductive substrates catalyse the redox-driven dissolution of Ag nanoparticles with the electrons transferred from the nanoparticles,via the macroscopic electrode to the dioxygen electron acceptor.

关键词： substrate electrocatalysis oxidative dissolution metal nanoparticles optical microscopy electrochemistry

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Applications of nanostructures in wide-field, label-free super resolution microscopy

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Chinese Physics B 2018年第11期27卷 186-195页

作者： Xiaowei Liu Chao Meng Xuechu Xu Mingwei Tang,Chenlei Pang Qing Yang State Key Laboratory of Modern optical Instrumentation College of Optical Science and Engineering Zhejiang University. Hangzhou 310027 China

Super resolution imaging capable of resolving details beyond the diffraction limit is highly desired in many scientific and application fields, including bio-medicine, nanomaterial science, and opto-electronic integration. Up to now, many different methods have been proposed, among which wide-field, label-free super resolution microscopy is indispensable due to its good applicability to diverse sample types, large field of view（FOV）, and high imaging speed. In recent years,nanostructures have made a crucial contribution to the wide-field, label-free subdiffraction microscopy, with various working mechanisms and configuration designs. This review summarizes the recent applications of the nanostructures in the wide-field, label-free super resolution microscopy, with the emphasis on the designs of hyperlens with hyperbolic dispersion, microsphere with ＂nano-jets＂, and nanowire ring illumination microscopy based on spatial frequency shift effect. The bottlenecks of the current techniques and possible solutions are also discussed.

关键词： optical microscopy spatial resolution nanowire metamaterials

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Deep learning facilitated whole live cell fast super-resolution imaging

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Chinese Physics B 2022年第4期31卷 757-763页

作者： Yun-Qing Tang Cai-Wei Zhou Hui-Wen Hao Yu-Jie Sun Institute of Systems Biomedicine School of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China Academy for Advanced Interdisciplinary Studies Peking UniversityBeijing 100871China State Key Laboratory of Membrane Biology Biomedical Pioneer Innovation Center(BIOPICBeijing 100871China School of Life Sciences Peking UniversityBeijing 100871China School of Future Technology Peking UniversityBeijing 100871China

A fully convolutional encoder-decoder network(FCEDN),a deep learning model,was developed and applied to image scanning microscopy(ISM).Super-resolution imaging was achieved with a 78μm×78μm field of view and 12.5 Hz-40 Hz imaging *** and dual-color continuous super-resolution images of microtubules and cargo in cells were obtained by *** signal-to-noise ratio of the obtained images was improved from 3.94 to 22.81 and the positioning accuracy of cargoes was enhanced by FCEDN from 15.83±2.79 nm to 2.83±0.83 *** a general image enhancement method,FCEDN can be applied to various types of microscopy *** with conventional spinning disk confocal microscopy was demonstrated and significantly improved images were obtained.

关键词： optical microscopy imaging and optical processing image processing

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