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DT-13, a saponin of dwarf lilyturf tuber, exhibits anti-cancer activity by down-regulating C-C chemokine receptor type 5 and vascular endothelial growth factor in MDA-MB-435 cells

引用

Chinese Journal of Natural Medicines 2014年第1期12卷 24-29页

作者： ZHAO Ren-Ping LIN Sen-Sen YUAN Sheng-Tao YU Bo-Yang BAI Xian-Shu SUN Li ZHANG Lu-Yong Jiangsu Center for Drug Screening China Pharmaceutical University Jiangsu Center for Pharmacodynamics Research and Evaluation China Pharmaceutical University Department of Complex Prescription of Traditional Chinese Medicine School of Chinese Material Medicine China Pharmaceutical University Department of Molecular Physiology University of Saarland Key Laboratory of Drug Quality Control and Pharmacovigilance China Pharmaceutical University Ministry of Education

AIM： To investigate the anticancer activity of DT-13 under normoxia and determine the underlying mechanisms of action. METHODS： MDA-MB-435 cell proliferation, migration, and adhesion were performed to assess the anticancer activity of DT-13, a saponin from ophiopogonjaponicus, in vitro. In addition, the effects of DT-13 on tumor growth and metastasis in vivo were evaluated by orthotopic implantation of MDA-MB-435 cells into nude mice; mRNA levels of vascular endothelial growth factor （VEGF）, C-C chemokine receptor type 5 （CCR5） and hypoxia-inducible factor 1a （HIF-1a） were evaluated by real-time quantitative PCR; and CCR5 protein levels were detected by Western blot assay. RESULTS： At 0.01 to 1 umol·L -1, DT-13 inhibited MDA-MB-435 cell proliferation, migration, and adhesion significantly in vitro. DT-13 reduced VEGF and CCR5 mRNAs, and decreased CCR5 protein expression by down-regulating HIF-1 a. In addition, DT-13 inhibited MDA-MB-435 cell lung metastasis, and restricted tumor growth slightly in vivo. CONCLUSION： DT-13 inhibited MDA-MB-435 cell proliferation, adhesion, and migration in vitro, and lung metastasis in vivo by reducing VEGF, CCR5, and HIF-la expression.

关键词： DT- 13 Saponin ophiopogonjaponicus Anticancer activity CCR5 VEGF HIF- 1 a

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Fingerprint analysis of Ophiopogonis Radix by HPLC-UV-ELSD coupled with chemometrics methods

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Journal of Chinese Pharmaceutical Sciences 2013年第1期22卷 55-63页

作者：李宁车彦云张梁张加余周渊姜勇屠鹏飞北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of 27 Ophiopogonis Radix samples were analyzed, and 18 reference substances were used for the identification of the common peaks. The fingerprint was further analyzed by chemometrics methods including similarity analysis （SA）, hierarchical clustering analysis （HCA） and principal component analysis （PCA）. The results indicated that the combination of chromatographic fingerprint and chemometrics analysis could be used for the geographical differentiation and quality evaluation of Ophiopognnis Radix.

关键词： Ophiopogonis Radix ophiopogonjaponicus HPLC-UV-ELSD Fingerprint Chemometrics Quality evaluation

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