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Functional characterization of seven terpene synthases from ophiopogon japonicus via engineered Escherichia coli

Science of Traditional Chinese Medicine 2024年第4期2卷 312-318页

作者： Wen Xu Qing Ma Tong Chen Haiyan Zhang Guanglei Ma Sibongile Mafu Juan Guo Xiaohui Fan Guanghong Cui Baolong Jin State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingChina College of Pharmaceutical Sciences Zhejiang UniversityHangzhouChina National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang UniversityJiaxingChina Department of Biochemistry and Molecular Biology University of Massachusetts-AmherstAmherstMAUnited States

Background:ophiopogon japonicus(L.f)*** in zhejiang is recognized as the Dao-di medicinal herb for the production of ophiopogonis ***-7-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside,a prominent pharmacologically active compound,serves as a marker distinguishing *** in zhejiang from those in other geographical *** is synthesized from borneol through glycosylation,with terpene synthase(TPS)being the critical enzyme catalyzing the conversion of terpene precursors into ***:The aim of the study was to define key genes involved in biosynthesis of borneol in ***:The candidate terpene synthase genes were identified from the root and leaf transcriptome data of *** in zhejiang and the functions of these enzymes were identified using engineered Escherichia ***:This study developed a rapid expression system for monoterpene and sesquiterpene synthases based on engineered *** terpene synthase genes(OjTPS1 to OjTPS7)were identified in different terpene synthase subfamilies,including 2 from TPS-a,4 from TPS-b,and 1 from *** analysis using an engineered system *** demonstrated that all the 7 terpene synthases produced monoterpenes,and OjTPS3,OjTPS5,and OjTPS6 also yielded ***:These 7 terpene synthases produced 13 monoterpenes and 8 ***,OjTPS1 produced borneol establishing the groundwork for elucidating the biosynthetic pathways of borneol-7-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside and other volatile oil components.

关键词： ophiopogon japonicus ophiopogon japonicus in zhejiang Terpene synthase Borneol

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