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Microbiological assessment of indoor air of a teaching hospital in Nigeria

Asian Pacific Journal of Tropical Biomedicine 2012年第6期2卷 465-468页

作者： Awosika SA Olajubu FA Amusa NA Department of Microbiology Olabisi Onabanjo University Department of Medical Microbiology & Parasitology Olabisi Onabanjo University Teaching Hospital Department of Plant Science & Applied Zoology Olabisi Onabanjo University

Objective:To investigate the quality of indoor air of different wards and units of Olabisi Onabanjo University Teaching Hospital, Sagamu, to ascertain their contribution to infection rate in the hospital.Methods:The microbial quality of indoor air of nine wards/units of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria was conducted. Sedimentation technique using open Petri-dishes containing different culture media was employed and samplings were done twice daily, one in the morning shortly after cleaning and before influx of people/patients into the wards/units and the other in the evening when a lot of activities would have taken place in these wards. Isolates were identified according to standard methods.Results:Results showed that there was a statistically significant difference(氈2= 6.0167) in the bacteria population of the different sampling time whereas it was not so for fungi population(氈2= 0.2857). Male medical ward(MMW) and male surgical general(MSG) recorded the highest bacterial and fungal growth while the operating theatre(OT) was almost free of microbial burden. The bacteria isolates were Staphylococcus aureus, Klebsiellasp., Bacillus cereus, Bacillus subtilis, Streptococcus pyogenes andSerratia marscenceswhile the fungi isolates includedAspergillus flavus, Penicilliumsp.,Fusariumsp.,Candida albicansandAlternariasp.Staphylococcus aureuswas the predominantly isolated bacterium whilePenicilliumsp. was the most isolated fungus.Conclusions:Though most of the microbial isolates were potential and or opportunistic pathogens, there was no correlation between the isolates in this study and the surveillance report of nosocomial infection during the period of study, hence the contribution of the indoor air cannot be established. From the reduction noticed in the morning samples, stringent measures such as proper disinfection and regular cleaning, restriction of patient relatives' movement in and out of the wards/units need to be enforced so as to improve the quality of indoor air of our hospital w

关键词： Indoor air OOUTH open-plate technique Nosocomial infections Staphylococcus aureus Microbiological assessment Microbial quality Bacterial isolate Fungal isolate Opportunistic pathogen Nosocomial infection Infection rate Microbial isolate Penicilliumsp Sedimentation technique

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Investigation of airborne fungi at different altitudes in Shenzhen University

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Natural Science 2010年第5期2卷 506-514页

作者： Li Li Chao Lei Zhi-Gang Liu 不详

Aim: To investigate the richness of species or genera of airborne fungi, the amount of airborne fungi, and its seasonal variation at different al-titudes in Shenzhen University. The effect of meteorological factors on airborne fungi was also analyzed. Methods: Slide-exposure me- thod and open-plate method were used. Results: There were 27 genera or species of fungus spores identified. Among the identified fungal genus, Cladosporium, Ustilago, Alternaria, Helminth-sporium and Uredinales were more prevalent. There were 18 genera of fungi colonies identified. Among which Penicillium, non-sporulating fungi, Aspergillus, Saccharomyces and Cladosporium were more common. The airborne fungal spores were present in the atmosphere of Shenzhen University all year round. The peaks of airborne spores appeared during April and October, while the lowest numbers were observed during January, July and December from March 2005-Febrary 2006. The highest volumes of fungi colonies were observed during April, October and September, while the lowest numbers were de-tected during in January, July and December or May from March 2005-Febrary 2006. The meteoro-logical factors had no relationship between the total monthly spore count at 10 and 30 meter height. At 70 meter, the total spores count was negatively correlated with solar radiation. Conclusions: Most of the fungi spores decreased along with the increase of altitudes.

关键词： Airborne Fungi open-plate Method Slide-Exposure Method

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