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两岸三地药理学与临床药理学术会议

作者： XIE Shu-wu~(1,2) ZHU Yan~(1,2) MA Li~(1,2) LI Zhi-ling~(1,2) GUI You-lun~(1,2) LU Ying-ying~3 ZHAO Zhi-fang~(1,2) CAO Lin~(1,2) (1. Department of Reproductive Pharmacology, Shanghai Institute of Planned Parenthood Research 2. National population and family planning key laboratory of contraceptive drugs and devices, Shanghai 200032, China 3. Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011,China)

Objective To establish a rat model of sterility associated with epididymis and epididymal gene ex- pression profiles relation to fertility by *** Rats were treated with 10 mg·kg·***-chlorohydrin for 10 consecutive *** maturation and other fertility parameters were ana- *** sperm motility and morphology were evaluated by computer-assisted sperm analysis (CASA);sperm survival rate was assessed by SYBR-14 and propidium iodide (PI) fluorescent staining;the weights of testes,epididymides,prostates and seminal vesicles were determined by electronic balance;histological exam- ination of above tissues were evaluated by HE staining;and serumal dihydrotestosterone (DHT) and testos- terone (T) of rats were detected by enzyme-labeled *** male rat was paired with 2 female rats in *** rats were examined the next morning for the presence of sperm in vaginal smears and underwent a cesarean section on clay 12 of *** the reproductive indices were calculated as follows:copulation index (number of sperm positive females/number of pairings),pregnancy index (num- ber of pregnancies/number of sperm positive females),and fertility index (number of pregnancies/num- ber of pairings).After that we used Affymetfix Rat 230 2.0 oligo-microarray to identify epididymal special genes associated with ***,we validated some of these genes by Real-Time quantitative poly- merase chain *** The motility of spermatozoa from the cauda epididymidis of treated rats showed a significant decrease in percentage of motile,progressively motile sperm,and sperm survival *** the same time,the morphology of cauda epididymal spermatozoa was also adversely affected by the treat- *** addition,the serumal androgen levels of treated animals weren’t changed compared with the con- trol ***,matings with treated males resulted in no successful ***,we classified general functions of the dow

关键词： male infertile rat ACH antifertility agents oligonucleotide microarray

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Gene Transcription Profile in Mice Vaccinated with Ultraviolet-attenuated Cercariae of Schistosoma japonicum Reveals Molecules Contributing to Elevated IFN-γ Levels

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 254-264页

作者： Xiang ZHU Zhao-Song ZHANG Min-Jun JI Hai-Wei WU Yong WANG Xiao-Ping CAI Lei ZHANG Shu-Ying HU Lin-Lin FU Feng LIU Chuan SU Guan-Ling WU Department of Pathogenic Biology,Nanjing Medical University.Nanjing 210029.China Department of Pathogenic Biology Nanjing Medical UniversityNanjing 210029 China

Vaccination with ultraviolet-attenuated cercariae of Schistosoma japonicum induced protectiveimmunity against challenge infection in experimental animal *** preliminary study on the transcrip-tion levels of IFN-γ and IL-4 in splenic CD4~+ T cells revealed that attenuated cercariae elicited predomi-nantly a Th1 response in mice at the early stage,whereas normal cercariae stimulated primarily Th2-dependent *** analysis on the gene profile of the skin-draining lymph nodes demonstratedthat the levels of IFN-γ were significantly higher in vaccinated mice than those in infected mice at day 4,7and 14 post-vaccination or ***,for IL-12 and IL-4,the potent inducers of Th1 andTh2 responses,respectively,as well as IL-10,there were no differences over the course of the experimentbetween the infected and vaccinated *** explore the underlying factors that may potentially contributeto elevated IFN-γ in vaccinated mice,the mRNA profiles of the skin-draining lymph nodes at day 4 post-exposure were compared using oligonucleotide *** the 847 probe sets with increased signalvalues,we focused on chemokines,cytokines and relevant receptors,which were validated by semi-quantitativeRT-PCR,A comprehensive understanding of the immune mechanisms of attenuated cercariae-inducedprotection may contribute to developing efficient vaccination strategies against ***,especiallyduring the early stare of infection.

关键词： Schistosoma japonicum attenuated cercariae oligonucleotide microarray IFN-γ chemokines

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Gene expression profile analyses of mice livers injured by Leigongteng

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World Journal of Gastroenterology 2007年第26期13卷 3619-3624页

作者： Yong Chen, Xiao-Ming Zhang Feng-Mei Han Peng Du Qi-Song Xia Hubei Provincial Key Laboratory of Traditional Chinese Medicine & Biotechnology School of Life Science Hubei University Wuhan 430062 Hubei Province China

AIM： To analyze the gene expression profiles of mice livers injured by Leigongteng and explore the relationship between the differentially expressed genes and liver damage. METHODS： The experimental mice were randomly divided into a control group and a liver-injured group in which the mice were administrated 33 μγ, of triptolide/ kg per day for 30 d. Liver mRNAs were extracted from animals in both groups and were reverse-transcribed to cDNA with dUTP labeled by different fluorescence （Cy3, Cy5） as hybridization probes. The mixed probes were hybridized with oligonucleotide microarray chips. The fluorescent signal results were acquired by scanner and analyzed with software. RESULTS： Among the 35852 target genes, 29 genes were found to be significantly differentially expressed, with 20 genes up-regulated and 9 genes down-regulated. The reliability of the differentially expressed genes was validated by RT-PCR experiments of 5 randomly selected differentially expressed genes. CONCLUSION： Based on the biological functions of the differentially expressed genes, it is obvious that the occurrence and development of liver damage induced by Leigongteng in mice are highly associated with immune response, metabolism, apoptosis and the cell skeleton of liver cells. This might be important for elucidating the regulatory network of gene expression associated with liver damage and it may also be important for discovering the pathogenic mechanisms of liver damage induced by Leigongteng.

关键词： Tripterygium Wilfordii Hook.f. Gene expre-ssion profile oligonucleotide microarray Mice

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Hepatic gene expression profiles associated with fibrosis progression and hepatocarcinogenesis in hepatitis C patients

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World Journal of Gastroenterology 2005年第13期11卷 1995-1999页

作者： Yujin Hoshida Motoyuki Otsuka Naoya Kato Ryosuke Tateishi Takuma Teratani Shuichiro Shiina Hiroyoshi Taniguchi Masaru Moriyama Takao Kawabe Masao Omata Department of Gastroenterology

AIM: To determine fibrosis progression and hepatocellular carcinoma (HCC), using simultaneous gene expression analysis. METHODS: Total RNA samples were extracted from liver biopsies from 19 patients with hepatitis C virus (HCV) infection and 3 patients without HCV infection. Among the 19 HCV-infected patients, 7 and 12 patients had grade Fl-2 and F3-4 fibrosis, respectively. Of the 12 patients with F3-4 fibrosis, 8 had HCC. Gene expression in the liver samples was determined using an oligonucleotide microarray. The following comparisons were performed: normal livers vs HCV-infected livers; F1-2 vs F3-4; and F3-4 with HCC vs F3-4 without HCC. Genes that were differentially expressed between these groups were identified based on signal-to-noise ratios. RESULTS: In the HCV-infected livers, genes involved in immune responses were highly expressed. Expression levels of genes for plasma proteins and drug-metabolizing enzymes were decreased and those of genes involved in the cell cycle and oncogenesis were increased in the F3-4 cases as compared to the F1-2 cases. Among the F3-4 cases, genes involved in carbohydrate metabolism tended to be more highly expressed in patients with HCC than in patients without HCC. CONCLUSION: We identified genes that are associated with fibrosis progression and hepatocarcinogenesis. This information may be used to detect increased carcinogenic potential in the livers of patients with HCV infection.

关键词： Hepatitis C Hepatocellular carcinoma oligonucleotide microarray

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Meta-analysis gene lists about subtypes of leukemia based on gene expression data

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Journal of Southeast University(English Edition) 2006年第2期22卷 248-255页

作者：杨锡南孙啸陆祖宏东南大学生物电子学国家重点实验室南京210096

To screen for molecular signatures that are commonly dysregulated in subtypes of a certain cancer, a novel meta-analysis is designed to perform rank score （RS） on lists of genes that are derived from different studies. RS is a promising way to detect signatures across platforms when integrating with one vs. all （OVA） or one vs. one （OVO） schemes of comparison. Among six published microarray expression datasets on acute leukemia, the biological signals hereafter provide stronger clustering support than systematic differences among microarray platforms. Moreover, the pediatric BCR_ABL specific genes can be used to correctly discriminate independent adult BCR ABL cases. The obtained results redound to discover, validate and treat the subtypes from microarray gene expression profiles of cancer, which have been plentifully researched, such as leukemia.

关键词： oligonucleotide microarray meta-analysis rank score leukemia

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Possible involvement of integrin signaling pathway in the process of recovery from restraint stress in rats

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Neuroscience Bulletin 2007年第4期23卷 229-235页

作者：高玉振郭试瑜印其章 Xiang—Qin CUI 久光正蒋星红苏州大学医学院神经生物学教研室苏州215123 苏州大学衰老与神经疾病实验室苏州215123 昭和大学医学部第一生理学教室东京142855日本阿拉巴马大学伯明翰分校生物统计学系阿拉巴马35294—0022美国

Objective To search novel genes or pathways involved in the recovery process after restraint stress in rats. Methods We compared the hypothalamus transcriptional profiles of two different recovery patterns （fast recovery vs slow recovery） from restraint stress in rats using oligonucleotide microarray, the recovery pattern was determined by the decrement of plasma adrenocorticotropic-hormone （ACTH） and corticosterone levels during one hour recovery period after stress. A real-time quantitative RT-PCR was applied to validate the differential expressed genes. Results Analysis of the microarray data showed that most of genes were not differentially expressed between fast recovery group and slow recovery group. Among the differentially expressed genes we found that talin, together with serine/threonine protein phosphatase PPl-beta catalytic subunit （PP-1B） and integrin α-6 precursor （VLA-6） genes, were at least 1.5 fold upregulated in the fast recovery group, while junctional adhesion molecule 1 （F11r） was 1.5 fold down-regulated in the fast recovery group. Conclusion The results implied that integrin signaling pathway may be involved in the recovery from restraint stress in rats. The present study provided a global overview of hypothalamus transcriptional profiles during the process of recovery from the restraint stress in rats. The integrin signaling pathway seems to be involved in the recovery process, which deserves further study to clarify the integrin-mediated recovery mechanism after restraint stress.

关键词： physical restraint adrenocorticotropic hormone corticosterone post-stress recovery oligonucleotide microarray

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Gene expression changes after hypoxic preconditioning in rat hepatocytes

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Hepatobiliary & Pancreatic Diseases International 2006年第3期5卷 416-421页

作者： Joan Rosello-Catafau Department of Experimental Pathology Instituto de Investigaciones Biomedicas de Barcelona CSICSpain

BACKGROUND: Hypoxic preconditioning can protect hepatocytes against hypoxic injury, but its mechanism has not been elucidated. The aim of this study was to profile gene expression patterns involved in hypoxic preconditioning and probable mechanism at the level of gene expression. METHODS: Hepatocytes were divided into 2 groups: control group and hypoxic preconditioning group. Biotinlabeled cRNA from the control group and the hypoxic preconditioning group was hybridized by oligonucleotide microarray. Genes that were significantly associated with hypoxic preconditioning were filtered, and validated at the level of transcript expression. RESULTS: Forty-three genes with significantly altered expression patterns were discovered and most of them had not been previously reported. Among these genes,genes encoding superoxide dismutase 2 (SOD2)and interleukin 10 (IL-10) in the hypoxic preconditioning group were confirmed to be up-regulated with real-time quantitative PCR. CONCLUSIONS: Many cytokines are involved in hypoxic preconditioning and protect hepatocytes from hypoxiareoxygenation injury, and the increase of oxygen freeradical scavengers and anti-inflammatory factors may play a key role in this phenomenon. Diverse signal pathways are probably involved.

关键词： hepatocyte hypoxic preconditioning oligonucleotide microarray superoxide dismutase 2 interleukin 10

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Investigation of molecular mechanism of CNS symptoms induced by DDVP poisoning

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世界急危重病医学杂志 2005年第3期2卷 702-706页

作者： ZHAO Zanmei WANG Hanbin Department of Internal Medicine Affiliated HospitalAcademy of Military Medical SciencesBeijing 100039P.R.China 不详

Objective To Screen differentially expressed genes related to dichlorphos(DDVP) poisoning from rat's hippocampus using oligonucleotide microarray technology in order to elucidate the mechanism of DDVP poisoning. Methods We composed probes of 40 genes of our interest. The probes were retrotranscribed on plata glass and oligonucleotide microarray was formed. 0.5 ml DDVP was given to the rats in experimental group and ***-pumping brine was given to the rats in control group by hypodermic injection, twenty minutes after convulsion, all hippocampus were collected for total RNA extraction, cDNAs were marked with Cy3 and Cy5 for control group and experiment group respectively, and hybridized with loligonucleotide microarray. Hybridization signals were collected and analyzed following scanning by laser co-focal scanner. Results There were 8 differentially expressed genes identified. Conclusion Many genes expressing changed by DDVP poisoning could be analyzed in a time period by using oligonucleotide microarray, which provides a powerful method for further studies on the molecular mechanism of DDVP poisoning.

关键词： DDVP hippocampus oligonucleotide microarray

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Identification of temporal responsive genes in human amnion epithelial FL cells induced by low concentration of N-methyl-N'-nitro-N-nitrosoguanidine

Identification of temporal responsive genes in human amnion ...

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2008年中国病理生理学会第十一届肿瘤和第十二届免疫专业委员会学术会议

作者： Hongjuan Li~(1,2) Jimin Shao~1 Xiangyun Lu~1 Yingnian Yu~(1*) 1 Department of Pathology and Pathophysiology,Zhejiang University School of Medicine,Hangzhou 310058,P.R.China 2 Department of Basic Medicine,Hangzhou Normal University,Hangzhou 310036,China Department of Basic Medicine,Hangzhou Normal University,Hangzhou 310036,China.

The alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) induces cellular DNA damage and other comprehensive alterations that lead to chromosomal aberrations,mutations, tumor initiation,and cell *** present s... 详细信息

关键词： N-methyl-N’-nitro-N-nitrosoguanidine Human amnion epithelial FL cells Differential gene expression oligonucleotide microarray Quantitative real-time RT-PCR

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Early transcriptional response induced by benzo(a)pyrene diol epoxide in human amnion epithelial FL cells

Early transcriptional response induced by benzo(a)pyrene dio...

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2008年中国病理生理学会第十一届肿瘤和第十二届免疫专业委员会学术会议

作者： Xiangyun Lu~§Jimin Shao~§Hongjuan Li Yingnian Yu~* Department of Pathology and Pathophysiology,Zhejiang University School of Medicine,Hangzhou 310058,P.R.China

(±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) is a carcinogen causing bulky-adduct DNA *** this study,we have investigated early transcriptional changes induced by various concentrations (0.005,0.05 and 0.5μM... 详细信息

关键词： (±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) Human amnion epithelial FL cells Early transcriptional response Differential gene expression oligonucleotide microarray Quantitative real-time RT-PCR

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