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Induction of Anthracnose Disease Resistance on Chili Fruit by Treatment of oligochitosan—Nanosilica Hybrid Material

Agricultural Sciences 2017年第10期8卷 1105-1113页

作者： Pham Dinh Dzung Le Thanh Hung Le Si Ngoc Hoang Dac Hiet Bui Van Le Nguyen Tien Thang Dang Van Phu Nguyen Ngoc Duy Nguyen Quoc Hien Research and Development Center for Hi-Tech Agriculture Ho Chi Minh City Vietnam Graduate University of Science and Technology Vietnam Academy of Science and Technology Hanoi Vietnam University of Science Vietnam National University Ho Chi Minh City Vietnam Research and Development Center for Radiation Technology Vietnam Atomic Energy Institute Ho Chi Minh City Vietnam

oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with the size of 10 - 30 nm was synthesized by calcination of acid treated rice husk. The mixture of 1% OC - 1% nSiO2 was prepared by dispersion of nSiO2 in OC solution. The morphology of nSiO2 in the mixture of OC-nSiO2 was measured from images of transmission electron microscopy (TEM). The effect of foliar application of the mixture of OC-nSiO2 on the induction of resistance against anthracnose disease caused by Colletotrichum gloeosporioides fungus on chili fruits was investigated. Results indicated that foliar application of OC-nSiO2 with the concentration of 60 mg/l - 60 mg/l was found to be as the optimal treatment that reduced the disease severity on chili fruits to 22.2% compared with 90.0% of the control. Thus, OC-nSiO2 hybrid material could be considered as an effective biotic elicitor to prevent anthracnose disease infection for chili fruits. Furthermore, the prepared OC-nSiO2 hybrid material can also be used as an environmentally friendly agrochemical product for sustainable development of agriculture.

关键词： oligochitosan Nanosilica Chili Fruit Colletotrichum gloeosporioides Anthracnose

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Studies on Solubility and Stability of oligochitosan in Effective Microorganisms

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Agricultural Science & Technology 2012年第3期13卷 623-626,629页

作者：潘国平吴国忠钟磊中国科学院上海应用物理研究所上海201800 嘉兴科瑞生物科技有限公司浙江嘉善3141003 浙江中科辐射高分子材料研发中心浙江嘉善314100

[Objective] This study aimed to explore the synergistic effect of oligochi- tosan and EM bacteria and provide a theoretical basis for its application in animal health, aquaculture, sewage treatment and other fields. [Method] Different concentrations of oligochitosan were added into EM bacteria liquid and the stability was preliminarily studied. [Result] Addition of oligochitosan with appropriate proportion had played a supporting role in the stable storage of EM bacteria liquid and had shown certain inhibition effect on flatulence of EM bacteria liquid. Addition of oligochitosan had no significant effect on the quality and re-fermentation of EM bacteria. [Conclusion] The study indicated that oligochitosan could be utilized in conjunction with EM bacteria liquid, having a promising practical potential application.

关键词： oligochitosan Effective microorganisms (EM) Stability

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Chitosan as A Preservative for Fruits and Vegetables:A Review on Chemistry and Antimicrobial Properties

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Journal of Bioresources and Bioproducts 2019年第1期4卷 11-21页

作者： Chao DUAN Xin MENG Jingru MENG MdIqbal Hassan KHAN Lei DAI Avik KHAN Xingye AN Junhua ZHANG Tanzina HUQ Yonghao NI College of Bioresources Chemical and Materials Engineering Shaanxi University of Science&TechnologyXi’an 710021China Department of Chemical Engineering University of New BrunswickFrederictonNB E3B 5A3Canada Tianjin Key Laboratory of Pulp and Paper Tianjin University of Science and TechnologyTianjin 300457China Faculty of Chemical Engineering Kunming University of Science and TechnologyKunming 650500China

Chitosan,derived from chitin,a major constituent(in quantity)of crustaceans,is a unique aminopolysaccharide with emerging commercial potential in agriculture,food,pharmaceuticals and nutraceuticals due to its nontoxic,biodegradable and biocompatable *** coating on fruits and vegetables has been found to be effective for the reduction of a variety of harmful micro-organims and extend the shelf-life of these *** this review,our focus is on the antimicrobial properties of chitosan and its application as a natural preservative for fresh *** detailed the key properties that are related to food preservation,the molecular mechanism of the antimicrobial activity of chitosan on fungi,gram-positive and gram-negative bacteria,coating methods for using chitosan and its formulation for preserving fruits and vegetables,as well as the radiation method of producing chitosan from *** the economic and scientific factors of chitosan’s production and efficiency as a preservative will open its practical application for fruits and vegetable preservation.

关键词： chitosan food preservative oligochitosan coating antimicrobial property

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Macrophage membrane coated ROS-responsive nanoparticles for the treatment of atherosclerosis

Macrophage membrane coated ROS-responsive nanoparticles for ...

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2018年第十二届中国药物制剂大会

作者：高成 Qiaoxian Huang Chonghui Liu Simon M.Y.Lee Ruibing Wang State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences University of Macau

Selected drug release to targeted tissues has been one of the most challenging tasks in drug delivery, which, if works as expected, would improve therapeutic efficacy and reduce side effects. In reality, the amount of drug released in targeted tissue is often far from adequacy, as most of the drugs are usually metabolized or eliminated during blood circulation before reaching targeted tissue.1 Recently, a variety of stimuli-sensitive drug delivery systems exhibiting selective drug release behaviors in response to the microenvironment in the specific tissues have been developed, however, few of these materials are able to achieve ideal targeting efficiency2. Herein, we report macrophage cell membrane coated ROS responsive nanoparticles（MM-NPs） based on oligochitosan. On the one hand, the membrane of immune cells significantly lowered the immunogenicity of the NPs and contributed to the targeted drug transport to inflammatory tissues where a high level of ROS is present. On the other hand, after reaching the targeted inflammatory tissue, the ROS sensitive NPs were broken apart and the encapsulated therapeutic payload was released in response to the high level of ROS. Transmission electron microscope（TEM） and dynamic light scattering（DLS） analysis exhibited a uniform nanosphere and good stability in PBS and cell culture medium. In vitro drug release profiles of the MM-NPs under various ROS conditions as well as in ROS-generating cellular model exhibited decent ROS-responsiveness. Furthermore, atorvastatin loaded NPs（MM-AT-NPs） improved the cell viability and lowered apoptosis rates of macrophage treated by lipopolysaccharide（LPS）, similar to those treated by free AT. The NO level and inflammatory cytokines were also attenuated on LPS treated macrophages by MM-AT-NPs. Finally, in vivo experiment on mouse showed significantly improvement on the accumulation of MM-NPs at the site of atherosclerosis in comparison to free drug and ROS responsive NPs. In summary, this cell m

关键词： atherosclerosis ROS-responsiveness macrophage oligochitosan

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