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Phytochemical Study and Evaluation of the Antiradical Activity of Extracts of oleaginous seeds of Panda oleosa and Isolona hexaloba from Gabon

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Food and Nutrition Sciences 2022年第2期13卷 165-180页

作者： M.-A. N’negue ép Mezui-Mbeng Darina Medza Prosper Edou Engonga Ognane Bikoro Lié Lyne Mengome Nestor Engone Abougone Sophie Laboratory of Chemistry-Biochemistry of the Faculty of Medicine University of Health Sciences Libreville Gabon Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA) National Center for Scientific and Technical Research (CENAREST) Department of Phytochemistry Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA) Centre National de la Recherche Scientifique et Technique (CENAREST) Département de Phytochimie Libreville Gabon Ecole Normale Supérieure Department of Physical Sciences Laboratoire Pluridisciplinaire des Sciences (LAPLUS) Libreville Gabon

Our study focused on phytochemical tests and evaluation of the anti-free radical activity of seed extracts of two oleaginous plants from Gabon used in traditional medicine or as condiments: Panda oleosa and Isolona hexaloba. The extraction was carried out by maceration with solvents of increasing polarity: cyclohexane, trichloroethylene, acetone, ethanol and finally distilled water. The total yields of the extracts are about 69.50% for Panda oleosa and 34.28% for Isolona hexaloba. The phytochemical tests carried out on the extracts of the seeds of Panda and Isolona highlight in both seeds the presence of alkaloids, polyphenols, triterpenes, carotenoids, reducing compounds, flavonoids, total sugars, coumarins, anthraquinones, free quinones, free anthracene derivatives, and terpenoids. Isolona seeds also contain leucoanthocyanins, sterols, cardiac glycosides and saponins. Phytochemical tests revealed the absence of tannins and mucilage in both seeds. The free radical scavenging activity was measured by scavenging the free radical cation of 2,2’-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS-+) with gallic acid as the reference antioxidant. The results of the free radical scavenging activity of the aqueous and ethanolic extracts of both seeds showed that the aqueous extracts were more active than the ethanolic extracts. The IC50s of the aqueous and ethanolic extracts of Panda seeds are 40 and 60 μg·mL-1 respectively, and those of the aqueous and ethanolic extracts of Isolona are 37.5 and 95 μg·mL-1 respectively. Gallic acid, the reference antioxidant (IC50 = 0.37 μg·mL-1) is about 10 times more active than the aqueous extracts of both seeds, 16 times more active than the ethanolic extract of Panda and 25 times more active than the ethanolic extract of Isolena.

关键词： Panda oleosa Isolona hexaloba oleaginous seeds Extracts Phytochemical Screening Antiradical Activity ABTS-+ Gallic Acid

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Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type

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Agricultural Sciences 2013年第7期4卷 32-44页

作者： Yah Gwladys Gnamien Irié Arsène Zoro Bi Yatty Justin Kouadio Yves Brostaux Jean-Pierre Baudoin Laboratory of Biology and Improving Vegetable Productions Natural Sciences UnitUniversity Nangui Abrogoua(UNA)AbidjanCote d’Ivoire Tropical Crop Husbandry and Horticulture Gembloux Agro-Bio Tech(GxABT)University of LiègeGemblouxBelgium Department of Applied Statistics Computer Science and MathematicsGembloux Agro-Bio Tech(GxABT)University of LiègeGemblouxBelgium

To regenerate adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were tested. Shoot induction proved to depend on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar allowed the production of numerous shoots without a callus phase. After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots with an average of 12.26 shoots per explant and a mean shoot length of 17.13 mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal parts ranged from 54% to 96%. Rooted plantlets were acclimatized and transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of the in-vitro-regenerated plantlets by means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets and mother plants.

关键词： AFLP Genetic Stability In Vitro oleaginous seeds Organogenesis

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