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Occurrence state and oil content evaluation of Permian Fengcheng Formation in the Hashan area as constrained by NMR and multistage Rock-Eval

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Petroleum Science 2023年第3期20卷 1363-1378页

作者： Yue Wang Xiang-Chun Chang Zhong-Quan Liu Peng-Fei Zhang Wei-Zheng Gao Zhi-Ping Zeng Gui-Sheng Li College of Earth Science and Engineering Shandong University of Science and TechnologyQingdaoShandong266590China Laboratoryfor Marine Mineral Reources Pilot National Laboratory for Marine Science and Technology QingdaoShandong 266071China Research Institute of Petroleum Exploration and Development Shengli Oil CompanySinopecDongyingShandong257001China No.5 Ooil Production Plant of Dagang oilfield Company CNPCTianjin300280China

The Hashan area,neighboring the Mahu Sag that is rich in the shale oil resources,showed commercial oil flow in the corresponding lacustrine shales of the Lower Permian Fengcheng Formation(P)with reserve scale approximately 789 million tons,presenting great potential for oil exploration.Despite their geographical proximity,the hydrocarbon occurrence and oil-bearing capacity of shale in the Hashan area and Mahu Sag greatly differ owing to the complex tectonic evolution.Therefore,understanding the occurrence state and oil content of the Pif in the Hashan area is crucial for ongoing shale oil exploration activities and the development of the northwestern margin of the Junggar Basin.In this study,an in-tegrated investigation,including petrological observations,scanning electron microscopy(SEM)obser-vation,analysis of nuclear magnetic resonance(NMR)Ti-T2 spectra,and conventional and multistage Rock-Eval pyrolysis methods were conducted to evaluate the occurrence state and oil content of the Pif shale in the Hashan area.The results indicate that plagioclase(average 30.7%)and quartz(24.1%)dominate the mineral compositions of the Pf shale samples.A method involving quartz-plagioclase-carbonate minerals is proposed to conduct lithofacies classification.In the Hashan area,the organic matter abundance in the Pf shale is scaled in fair to good range,the thermal maturity ranges from immature to early mature stage,and the primary organic matter types are Types I and Ilj.Intergranular and dissolution pores are the two most common pore types.The free oil is mostly found in the pores and microfractures of the mineral matrix,whereas the adsorbed oil is mostly adsorbed on the surfaces of kerogen and clay minerals.The high organic matter abundance,quartz content,and porosity account for substantial increase in the oil content,the area rich in shale oil resources coincides with that rich in free oil.The most favorable lithofacies in the Hashan area is the calcareous mudstone/shale,which hosts the highest free oil content(average 2.49

关键词： Hashan area oil content Occurrence state Pore types Ti-T2 NMR Shale oil

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The key parameter of shale oil resource evaluation: oil content

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Petroleum Science 2022年第4期19卷 1443-1459页

作者： Min Wang Ming Li Jin-Bu Li Liang Xu Jin-Xu Zhang State Key Laboratory of Shale oil and Gas Enrichment Mechanisms and Effective Development Beijing102206China SINOPEC Key Laboratory of Petroleum Accumulation Mechanisms WuxiJiangsu 214126China Shandong Provincial Key Laboratory of Deep oil and Gas QingdaoShandong 266580China School of Geoscience China University of Petroleum(East China)QingdaoShandong 266580China

The United States has become the world's largest oil producer of shale oil. China has abundant shale oil resources, but its resource potential has not yet been exploited. The core of the evaluation is the selection of parameters and their reliability. By combining the parameters of the shale oil resource evaluation, we investigated the key parameters in the evaluation model and reviewed the research results. The adsorption and retention of heavy hydrocarbons, loss of light hydrocarbons, and original oil saturation are key in the evaluation of shale oil resources. The adsorption and retention of heavy hydrocarbons can be determined by the pyrolysis, FID curve, and hydrocarbon generation kinetics of shale before and after extraction. The loss of light hydrocarbons mainly occurs in coring(change in temperature and pressure),sample treatment, which can be evaluated using the GC spectrum, rock pyrolysis, crude oil volume coefficient, mass balance, component hydrocarbon generation kinetics, and other methods. The original oil saturation evaluation includes indirect, direct, logging, and simulation methods. The most reliable parameters can be obtained by using the sealed or pressure-maintained coring immediately after thawing(without crushing), and the recovery of light hydrocarbon loss is critical for the resource evaluation of medium to high mature shale. Therefore, the experimental determination of shale oil content and the study of the influencing factors of the parameters should be strengthened.

关键词： Shale oil oil content oil saturation Light hydrocarbon recovery Resource evaluation

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Refinement of four major QTL for oil content in Brassica napus by integration of genome resequencing and transcriptomics

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The Crop Journal 2022年第3期10卷 627-637页

作者： Shuxiang Yan Huaixin Li Hongbo Chao Jianjie He Yiran Ding Weiguo Zhao Kai Zhang Yiyi Xiong Kang Chen Libin Zhang Maoteng Li Department of Biotechnology College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074HubeiChina Key Laboratory of Molecular Biophysics of the Ministry of Education Wuhan 430074HubeiChina School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001HenanChina

Rapeseed(Brassica napus)supplies about half of the vegetable oil in China.Increasing oil production and searching for genes that control oil content in the crop are research goals.In our previous studies,four major QTL for oil content located on A08,A09,C03 and C06 in the Ken C-8×N53-2(KN DH)mapping population were detected.The parental lines were resequenced to identify structural variations and candidate genes affecting oil content in these four major QTL regions.Insertion-deletion(In Del)markers were developed and used to narrow the regions.Differentially expressed genes located in the regions were investigated.GO and KEGG analysis showed that several genes were associated with lipid metabolism.Several transcription factors with higher expression in N53-2 than in Ken C-8 were identified.These results shed light on the genetic control of oil content and may be helpful for the development of highoil-content cultivars.

关键词： Brassica napus oil content QTL mapping Resequencing Transcriptome

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Profiling and geographical distribution of seed oil content of sunflower in Ukraine

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oil Crop Science 2023年第2期8卷 111-120页

作者： Vasyl Petrenko Andriy Topalov Liudmyla Khudolii Yuliia Honcharuk Valeria Bondar Department of Grain Technologies Institute of Food Resources NAASKyivUkraine Department of Computerized Control Systems of the Admiral Makarov National University of Shipbuilding MykolaivUkraine Department of Application Acceptance Institute of Plant Variety ExaminationKyivUkraine Scientific Secretary Institute of Horticulture NAAS KyivUkraine Department of Ecology National University of Life and Environmental SciencesKyivUkraine

The sunflower has the potential to be a highly competitive crop owing to its diversity market and the valuable oil it produces.Nevertheless,the amount of sunflower seed oil-a key factor for the crushing industry-is affected by genotype and environment,which sometimes is difficult to predict.The paper analyzes the theoretical principles of sunflower growth in Ukraine,highlights the current trends in the sowing of this crop,the influence of climatic factors of the environment on the prospects of the oilseed sunflower yield.The results of the research are presented for 3 years(2014-2016),during the authors conducted a grain quality observatory at the regional level,taking into account the climatic conditions of sunflower growth in different natural zones of Ukraine(from 45to 51north latitude and from 22to 37east longitude).Additionally,the research includes the coefficient of significance of the deviation from long-term climatic indicators,which makes it possible to forecast sunflower yield in the future.The analyzed data was obtained from the commercial fields with a minimum area of 40 ha in all Ukrainian regions except Crimea peninsula,Donetsk and Luhansk regions.The results obtained in research show the great diversity of oil content index as per both latitude and longitude meaning.If we consider the entire country,this survey shows that the seed oil content can change dramatically from year to year:e.g.47.5%in one year versus 45.7%in another year.Regionally,these changes have a completely different dynamic with a gradual decrease in seed oil content in the southern part of the country.The main goal of the research was to identify the best sunflower growing areas in Ukraine according to the oil content index.

关键词： Sunflower seeds oil content Longitude Latitude Location

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Effect of Spraying with GA<sub>3</sub>on the Quality and oil content of Syrian Okra Seeds <i>Abelmoschus esculentus</i>(L.)

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American Journal of Plant Sciences 2022年第1期13卷 83-90页

作者： Dima Zolfikar Khrmashow Miteadi Bouras Fahed Sahuni Department of Horticulture Faculty of Agriculture Tishreen University Lattakia Syria Department of Horticulture Second College of Agriculture Aleppo University Aleppo Syria

This study aimed to estimate the effect of spraying of okra plants, Abelmoschus esculentus (L.) with GA3 on the quality and oil content of the seeds. For this, the local okra variety was selected and used, which was growing widespread in the coastal region of Syria. This study was carried out in Al-Haffa area, Lattakia governorate, Syria (with 105 MSL elevation) for two seasons 2019-2020. The results showed that the effect of spraying with GA3 was more evident in the quality of the seeds as well as oil content at 75 ppm concentration with 68.7 g weight of 1000 seeds and 5.31% seed oil percentage. This study also showed that oil of local okra variety seed was rich in the essential fatty acids namely, linoleic, palmitic, oleic, stearic and linolenic, treatment with 75 ppm concentration of GA3 was more effective in the seed content of essential fatty acids with 42.30%, 32.55%, 9.20%, 6.73% and 5.72%, respectively. Finally, our results were indicated that treatment of okra plants at 75 ppm of GA3 enhanced the quality and oil content of seeds compared to the other concentrations.

关键词： oil content GA3 Fatty Acids Linoleic Palmitic Okra

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An Integrated Quantitative Trait Locus Map of oil content in Soybean,Glycine max(L.) Merr.,Generated Using a Meta-Analysis Method for Mining Genes

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Agricultural Sciences in China 2011年第11期10卷 1681-1692页

作者： QI Zhao-ming HAN Xue SUN Ya-nan WU Qiong SHAN Da-peng DU Xiang-yu LIU Chun-yan JIANG Hong-wei HU Guo-hua CHEN Qing-shan Soybean Research Institute Northeast Agricultural University Harbin 150030 P.R. China Crop Research and Breeding Center of Land-Reclamation Harbin 150090 P.R.China Heilongjiang Academy of Agricultural Sciences Suihua Institute Suihua 152052 P.R.China

Soybean is a major cash crop in the world, and its oil content was one of the very important traits. Therefore, the study of gene mapping for oil content in soybean is very important for breeding application. At present, at least 130 QTL loci for soybean oil content have been published; however, the mapping results of oil content were dispersed and a coalescent public map should be established to integrate the published QTLs, and to more efficiently mine genes based on the meta- analysis method of the bioinformatics tools. This study was to construct an integrated map of QTLs for soybean oil content and accelerate the application of bioinformation resource related to oil content improvement in the practice of soybean breeding. We collected information of 130 QTLs reported over the past 20 yr for soybean oil content and used the Software BioMercator 2.1 to project QTLs from their own maps onto a reference map, which was an early-integrated map constructed by Song （2004） for oil-content quantitative trait loci （QTLs） in soybean. Gene mining was performed based on the meta-analysis by running the local ver. GENSCAN and InterProScan. The confidence interval of QTLs was efficaciously narrowed using the meta-analysis method, and 25 consensus QTLs were mapped on the reference map. Using a local version of GENSCAN, 12 805 sequences in the consensus QTL intervals were predicted. With BLAST, these predicted sequences were aligned to gene sequences from the International Protein Index database using InterProScan locally. Thirteen predicted genes were in the class of the geme ontology （GO） accession （0006631）, which were involved in the fatty acid metabolic process. These genes were analyzed using BLAST at the NCBI website to examine whether they were related to oil content. Six genes were found in the oil-synthesis pathway. Twenty-five consensus QTLs and six genes were found in the oil-synthesis pathway. These results would lay the foundation for marker-assisted selection and mapping QTL precisely, and these genes will faci

关键词： soybean oil content meta-analysis consensus QTL gene ontology （GO）

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Moisture sorption and thermodynamic properties of Camellia oleifera seeds as influenced by oil content

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International Journal of Agricultural and Biological Engineering 2021年第1期14卷 251-258页

作者： Guangfei Zhu Qi Jin Yanhong Liu Yawen Lin Jun Wang Xingyi Li College of Engineering China Agricultural UniversityBeijing 100083China School of Pharmacy Gannan Medical UniversityGanzhou 341000JiangxiChina

Moisture sorption isotherms and thermodynamic properties of Camellia oleifera seeds as influenced by oil content were investigated.Moisture desorption and adsorption isotherms of Camellia oleifera seeds,kernels and shells from three varieties were determined using constant temperature and humidity chamber method at different temperatures(10°C,25°C,and 40°C)with water activity ranging from 0.20 to 0.90.Six selected mathematic models were employed to fit the experimental data.The Peleg model gave the best results for both seeds and kernels and Langmuir model was the best for shells.The difference of equilibrium moisture contents at the same water activities during desorption and adsorption indicated the occurrence of hysteresis of adsorption processes and the equilibrium moisture contents tended to decrease with the increasing oil content and temperature.The binding energy and average capacity per unit mass decreased with increasing temperature and oil content.The relationships between water activity and the logarithm of sorption activity showed the capillary porous body characteristics of the seeds.

关键词： Camellia oleifera seeds moisture desorption and adsorption isotherms equilibrium moisture content oil content thermodynamic property

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QTL Analysis of the oil content and the Hull content in Brassica napus L.

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Agricultural Sciences in China 2007年第4期6卷 414-421页

作者： JIN Meng-yang LI Jia-na FU Fu-you ZHANG Zheng-sheng ZHANG Xue-kun LIU Lie-zhao College of Agronomy and Biochnology Southwest University Beibei 400715 P.R.China oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 P.R.China

The QTLs of the oil content and the hull content were analyzed in Brassica napus L. by constructing the linkage map. The F2：6RIL population with 188 lines, derived from the cross of GH06 × P147, was used as the mapping population. The SRAP, SSR, AFLP, and TRAP markers were used to construct the linkage map, and the composite interval mapping （CIM） to identify the quantitative trait loci associated with the oil content and the hull content. 300 markers were integrated into 19 linkage groups, covering 1 248.5 cM in total. Seven QTLs were found to be responsible for the oil content with the single contribution to phenotypic variance ranging from 3.73 to 10.46%; four QTLs were found for the hull content with the single contribution to phenotypic variance ranging from 4.89 to 6.84%. The yellow-seeded Brassica napus L. has the advantage of higher oil content and the hull content has a significant effect on the oil content. In addition, the SRAP marker is good for detecting QTL.

关键词： Brassica napus L. genetic map molecular markers QTL oil content hull content

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Progress on genetic and genomic research for enhanced oil content and quality in peanut

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oil Crop Science 2016年第1期1卷 1-8页

作者： Boshou LIAO Yong LEI Li HUANG Silong CHEN Xiaoping REN Liying YAN Yurong LI Dongxin HUAI Xiaojing ZHOU Liyun WAN Yuning CHEN Wei HUA Huifang JIANG oil Crops Research Institute of Chinese Academy of Agricultural Sciences Wuhan 430062 P. R. China Cereal and oil Crops Research Institute of Hebei Academy of Agricultural and Forestry Sciences Shijiazhuang 050035 P. R. China

Peanut or groundnut (Arachis hypogaea L.) is an important source of vegetable oil in the world.Genetic enhancement for high yield and high oil content has greatly contributed to enhanced productivity of peanut and increased supply of peanut oil.Further improving oil content and quality of peanut is still crucial for increasing productivity of arable land and market competitiveness of peanut oil.Based on investigation among the peanut germplasm accessions including wild Arachis species,the oil content could be as high as 65％.Heterosis has been observed for oil content in hybrids derived from diverse crossing parents.Segregates with enhanced oil content have been obtained by pyramiding different genes or alleles with major and minor additive effects.Improved testing techniques for oil content in breeding lines with reduced cost have accelerated breeding progress for high oil content.SSR markers associated with oil content have been identified by association and linkage analysis.The stability of oil content in peanut across seasons and locations is highly associated with cold tolerance,high nutrition efficiency and drought tolerance.Recent progress on improving fatty acids in particular marker assisted backcrossing breeding has contributed to higher quality peanut oil and other products.High oil peanut lines with improved resistance to aflatoxin production have been developed.The wild Arachis species would be of great value for peanut breeding in increasing oil content.

关键词： Arachis hypogaea L. wild Arachis species oil content oleic acid fatty acid components resistance to aflatoxin production heterosis marker assisted breeding

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Heterosis for Seed Yield, oil content and Other Characters in Rapeseed (Brassica napus L.)

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Journal of Northeast Agricultural University(English Edition) 2010年第1期17卷 1-9页

作者： HUANG Zesu LAOSUWAN Paisan MACHIKOWA Thitipom CHEN Zehui Guizhou Academy of Agricultural Sciences Guiyang 550006 China School of Crop Production Institute of Agriculture Technology Suranaree University of Technology Nakhon 30000 Ratchasima Thailand Faculty of Science and Technology Hatyai University Hatyai 90110 Songkla Thailand

Nine inbred lines of rapeseed （Brassica napus L.） used as male were crossed to five recessive genetic male sterile （RGMS） lines used as female to produce 45 single crosses. The crosses, their parents and a check hybrid were tested at two locations during 2007 to 2008 for testing the performance and heterosis of hybrids for seed yield and other characters. Results showed that variations for seed yield, oil content, days to flowering and days to maturity were significant. Mean squares for hybrids were significant for all characters. High heterosis （-4.5%-88.3%）, heterobeltiosis （-15.6%-81.1%） and standard heterosis G34.8%-33.1%） were found for the seed yield. The highest heterosis and heterobeltiosis were found in the cross QH303-4A× 1190. The highest standard heterosis was found in the cross Qianyou8A×Q034. Both positive and negative heterosis of single crosses were detected for the oil content. Small heterosis was found for days to flowering and days to maturity. Among parents, Ⅲ 188, Ⅲ224, and Q034 were proved to be the superior for seed yield when used as parents in most of the hybrid combinations. 2365, Ⅲ224, and QH303-4AB were good for high oil content breeding, Ⅲ 176, 2313 and Qianyou3AB were good for the early hybrid breeding. Total 11 hybrids yielded higher than 2 500 kg·hm^-2 and also gave significantly positive heterosis, heterobeltiosis and standard heterosis. Among them, 10 crosses gave higher oil content than that of check. These crosses can be used in the future breeding program for the seed yield and the oil content. Two crosses including Qianyou3A × Ⅲ224 and Qianyou3A×2313 can be used for the early breeding program.

关键词： Brassica napus L. yield oil content heterosis

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