检索结果-南通市图书馆

Taste masking of ofloxacin and formation of interpenetrating polymer network beads for sustained release

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2017年第4期7卷 244-251页

作者： A. Michael Rajesh Kiritkumar Mangaldas Popat Reverse Osmosis Membrane Division Central Salt and Marine Chemicals Research InstituteCouncil of Scientific & Industrial Research (CSIR)G.B. MargBhavnagar 364 002India Academy of Scientific and Innovative Research Central Salt and Marine Chemicals Research InstituteCouncil of Scientific & Industrial Research (CSIR)G.B. MargBhavnagar 364 002India

The objective of this study was to carry out taste masking of ofloxacin(Ofl) by ion exchange resins(IERs)followed by sustained release of Ofl by forming interpenetrating polymer network(IPN) beads. Drug-resin complexes(DRCs) with three different ratios of Ofl to IERs(1:1, 1:2, 1:4) were prepared by batch method and investigated for in vivo and in vitro taste masking. DRC of methacrylic acid-divinyl benzene(MD) resin and Ofl prepared at a ratio of 1:4 was used to form IPN beads. IPN beads of MD 1:4 were prepared by following the ionic cross-linking method using sodium carboxymethyl xanthan gum(SCMXG) and SCMXG-sodium carboxymethyl cellulose(SCMXG-SCMC). IPN beads were characterized with FT-IR and further studied on sustained release of Ofl at different pH. In vivo taste masking carried out by human volunteers showed that MD 1:4 significantly reduced the bitterness of Ofl. Characterization studies such as FT-IR, DSC, P-XRD and taste masking showed that complex formation took place between drug and resin. In vitro study at gastric pH showed complete release of drug from MD 1:4 within 30 min whereas IPN beads took 5 h at gastric pH and 10 h at salivary pH for the complete release of drug. As the crosslinking increased the release kinetics changed into non-Fickian diffusion to zero-order release mechanism. MD 1:4 showed better performance for the taste masking of Ofl and IPNs beads prepared from it were found useful for the sustained release of Ofl at both the pH, indicating a versatile drug delivery system.

关键词： Ion exchange resins ofloxacin in vivo & in vitro taste masking Interpenetrating polymer network Sustained release

Cuprous Oxide/Nitrogen-doped Graphene Nanocomposites as Electrochemical Sensors for ofloxacin Determination

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2016年第3期32卷 468-473页

作者： WU Fanghui XU Fan CHEN Le JIANG Binbin SUN Wenbin WEI Xianwen School of Chemistry and Chemical Engineering Anhui University of Technology Maanshan 243002 P. R. China

Cu2O/nitrogen-doped grapheme（NG） nanocomposite material was prepared via a facile one step chemical reduction and characterized by means of X-ray diffraction（XRD） and scanning electron microscopy（SEM）. A new electrochemical sensor was then fabricated by coating Cu2O/nitrogen-doped graphene nanocomposite with Nation on glassy carbon electrode（Cu2O/NG/Nation/GCE）. The electrochemical response of this modified electrode toward of- loxacin was examined by cyclic voltammetry. The results indicate that Cu2O/NG/Nafion composite-modified elec- trode exhibits higher catalytic activity in the electrochemical oxidation of ofloxacin compared with glassy carbon electrode（GCE）, Cu2O/Nafion modified electrode（Cu2O/Nafion/GCE）, and N-doped graphene/Nation modified electrode（NG/Nafion/GCE）. Under optimal conditions, the peak current was found to be linearly proportional to the concentration of ofloxacin in the 0.5-27.5 μmol/L and 27.5-280 μmol/L ranges with a lower detection limit of 0.34 μmol/L, higher sensitivity of 39.32 μA-L-mmoV1 and a shorter reaction time of less than 2 s. In addition, Nation can enhance the stability of the modified electrode and prevent some negative species. Thus the modified electrode exhibits good selectivity and a long working life. The Cu2O/NG/Nafion composite modified electrode shows promising application in electrochemical sensors, biosensors, and other related fields because of its excellent properties.

关键词： Cu2O Nitrogen-doped graphene ofloxacin Electrochemical method Determination

Highly sensitive trivalent copper chelate-luminol chemiluminescence system for capillary electrophoresis chiral separation and determination of ofloxacin enantiomers in urine samples

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2014年第6期4卷 412-416页

作者： Hao-Yue Xie Zuo-Rong Wang Zhi-Feng Fu Key Laboratory of Luminescence and Real-Time Analysis (Ministry of Education) College of Pharmaceutical Sciences Southwest University Zigong Institute for Food and Drug Control

A simple,fast and sensitive capillary electrophoresis（CE） strategy combined with chemiluminescence（CL） detection for analysis of ofloxacin（OF） enantiomers was established in the present work.Sulfonated p-cyclodextrin（β-CD） was used as the chiral additive being added into the running buffer of luminol-diperiodatocuprate（Ⅲ）（K[Cu（HIO6）2],DPC） chemiluminescence system.Under the optimum conditions,the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits（S/N=3） of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin,respectively.The linear ranges were both 0.010-100 μM.The method was utilized for analyzing OF in urine;the results obtained were satisfactory and recoveries were 89.5-110.8%,which demonstrated the reliability of this method.This approach can also be further extended to analyze different commercial OF medicines.

关键词： ofloxacin Chiral analysis Sulfonated-β-CD Capillary electrophoresis Chemiluminescence

In vitro and in vivo evaluation of gastroretentive floating drug delivery system of ofloxacin

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Pharmaceutical Sciences 2013年第3期8卷 191-198页

作者： Rajani Shaky Panna Thapa Ranendra N.Saha Department of Pharmacy Kathmandu UniversityP.O.Box:6250DhulikhelKavreNepal Birla Institute of Technology and Science PilaniRajasthanIndia

This study aimed to develop hydrophilicmatrix based controlled release gastroretentive drug delivery system of ofloxacin and conducting its in vitro and in vivo evaluations.Effervescent floating gastroretentive drug delivery system of ofloxacin was prepared utilizing Boxe Behnken statistical design with 3 factors,3 levels and 15 experimental trials.Formulation optimization was done by setting targets on selected responses.In vivo studies were carried out for the optimized formulation with 12 healthy human volunteers and obtained pharmacokinetic parameters were compared with themarketed once daily formulation,“Zanocin OD”.Optimized formulation showed satisfactory controlled in vitro drug release for more than 12 h with excellent buoyancy properties(floating lag time16 h).Optimized and marketed formulations were found to have similar in vitro release profile(f2¼79.22)and also were found to be bioequivalent.Serum ofloxacin concentration was well maintained above its reported minimum inhibitory concentrations for most of the pathogens for sufficiently longer duration.Cmax and AUC values of optimized formulation were found to be significantly higher than of marketed product despite their bioequivalence.Bettertherapeutic effect can be expected since ofloxacin exhibits concentration dependent killing.Hence,gastroretention can be a promising approach to enhance bioavailability of ofloxacin with narrow absorption window in upper GIT.

关键词： ofloxacin Gastroretentive drug delivery system BoxeBehnken design Bioavailability Floating

Statistical modeling of radiolytic (6OCo γ) degradation of ofloxacin, antibiotic: Synergetic effect, kinetic studies & assessment of its degraded metabolites

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2019年第3期13卷 147-161页

作者： G. S. Muthu Iswarya B. Nirkayani A. Kavithakani V. C. Padmanaban Centre for Research Department of Biotechnology Kamaraj College of Engineering & Technology Madurai Tamilnadu India

The impact of human activities in the past few decades has paved the way for the release of pollutants due to the improper effluent treatment. Recent studies revealed that, ofloxacin, an antibiotic as one of the major pollutant affecting surface water and ground water. In this study, the radiolytic potential of ofloxacin was investigated. The effects of pH, dose and concentration of ofloxacin were analyzed using One Factor At a Time (OFAT) and the interactive effects between the parameters were studied using Face Centered Central Composite Design. The statistically optimised developed model shows 30% degradation at initial antibiotic concentration of ImM at pH 3.0 and at 2 kGy dose of gamma ray. The process efficiency was evaluated in terms of G value and its correlation with the concentration of antibiotic was also established. The process of degradation was augmented by the addition of H2O2 (1.5 mM). The reaction kinetics for the process was evaluated, the dose rate constant and the rate of degradation for the augmented process was found to be 0.232 kGy^-1 and 0.232 mM/kGy, respectively. The degraded metabolites of the radiolytic degradation of ofloxacin were analyzed through change in pH, reduction in TOC and GC-MS spectrum.

关键词： ofloxacin Gamma irradiation Face centered central composite design Reaction kinetics Gas Chromatography-Mass spectrum

Fly ash and zero-valent iron-based in situ advanced anaerobic digestion with emphasis on the removal of antibiotics and antibiotic resistance genes from sewage sludge

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Waste Disposal and Sustainable Energy 2022年第1期4卷 17-28页

作者： Minquan Zhang Yadan Wangjin Haidong Zhou Ziming Zhao Zhengcao Cao Zhenxi Ying School of Environment and Architecture University of Shanghai for Science and TechnologyShanghai 200093China Shanghai Technical Institute of Electronics Information ShanghaiChina

This study investigated the removal of antibiotics and antibiotic resistance genes from sewage sludge by using fly ash(FA)-based in situ advanced anaerobic digestion(AAD)under mesophilic conditions.Five antibiotics(sulfadiazine,sulfameth-oxazole,ofloxacin,tetracycline,and roxithromycin)and 11 corresponding antibiotic resistance genes(Ib-cr,qnrS,ermF,ermT,ermX,sul1,sul2,sul3,tetA,tetB,and tetG)were selected as the targets.Adding FA to anaerobic digestion to remove antibiotics and resistance genes allows waste to be treated with waste.FA-based in situ AAD of sewage sludge effectively enhanced the process stability and methane yield,and the optimal FA-added dosage was 50 mg/L.The cumulative methane yield could be well described with the improved Gompertz model.FA addition effectively increased the overall removal of ofloxacin,by up to 85.3%at 50 mg/L FA and 10μg/L antibiotics,and the combination of zero-valent iron and FA enhanced only the overall removal of ofloxacin to 92.4%and tetracycline to 85.6%.However,FA-based in situ AAD could not enhance the overall removal of other antibiotics from sewage sludge.Not all the same types of antibiotic resistance genes were strongly positively correlated with the concentrations of antibiotics.The removal of antibiotic resistance genes influenced by FA addition varied largely with the types of antibiotic resistance genes,FA dosage,antibiotic content,and the combination with zero-valent iron.FA addition could not be verified to enhance the removal of antibiotic resistance genes.The addition of FA or zero-valent iron and the antibiotic concentrations significantly changed the microbial community structure during in situ AAD,and the combination of zero-valent iron and FA significantly reduces the species diversity and microbial abundance.The most abundant bacteria were Methanogarcina,Methanoberium,unidentified_Archaea,Terrimonas,Methomethoxychlo-rovorans,and Candidatus_Cloacimonas in the ZVI-FA test.

关键词： Biogas production Methane yield ofloxacin Relative abundance Microbial community structure

TNF /TNFR1 signaling pathway and endocytoplasmic reticulum stress (ERs) mediated apoptosis are involved in ofloxacin and marbofloxacin-induced apoptosis of juvenile dog joint chondrocytes in early stage in vitro.

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

畜牧与兽医 2012年第S2期44卷 46-47页

作者： Fu-Tao Zhang Dan Xing Ming-Xing Ding College of Veterinary Medicine Huazhong Agricultural University

Quinolones (QNs) are widely used for their broad antibacterial spectrum and good antibacterial activities,desirable pharmacokinetic characteristics and few cross reactions with other therapeutic agents. However,QNs have adverse effects including chondrotoxicity in juvenile animals,so the use of QNs is contraindicated in children and adolescents. In regarding to the chondrotoxicity of QNs,numerous studies have been done. The current hypothesis suggests that QNs compete with the β1 integrin receptors residing on chondrocyte surface for extracellular Mg ions,which leads to alternation in β1 integrin expression,or function and eventually results in chondrocyte death. Stupack et al (2001)demonstrated that caspase-8 could be recruited to unligated integrins in adherent cells and further initiate apoptosis in a death receptor-independent manner. Sheng et al (2008) found that ofloxacin induced rabbit's chondrocyte apoptosis by causing disturbance of β1 integrin functions and subsequently through caspase-8-dependent mitochondrial pathway.Apoptosis could be initiated through the stimulation of death receptors and through an intrinsic pathway from mitochondria. Santangelo and Bertone (2011) and Wu et al (2011) found that TNFα could be expressed in human primary condrocytes inducing by interleukin-1beta (IL-1) or lipopolysaccharide (LPS). However,to date there have not been sufficient results to support that signaling from the death receptors was involved in QNs-induced chondrocyte apoptosis.In addition,dilated cisternae of rough endoplasmic reticulum (ER) have been noticed in QNs-induced arthropathy.ER can participate in the initiation of apoptosis. Varieties of harmful cellular stimuli could lead to ERs (ER stress). Elevated ERs results in cellular apoptosis. But whether ERs mediates apoptosis in QNs-treated chondrocytes is not clear yet.In this study,we chose two QNs agents and chondrocytes were treated with ofloxacin and marbofloxacin at final concentrations of 20 μg / mL,50 μg / mL and 100 μg / mL respectively in vitro

关键词： ofloxacin marbofloxacin TNF/TNFR1 ERs apoptosis

Synthesis and Characterization of Soy Protein Isolate/MMT Nanocomposite Film for the Control Release of the Drug ofloxacin

在线全文

学校读者我要写书评

暂无评论

World Journal of Nano Science and Engineering 2011年第2期1卷 27-36页

作者： Preetishree Nayak Sanjib Kumar Sahoo Anamika Behera Prativa Kumari Nanda P.L. Nayak B.C. Guru 不详

Nanocomposites were prepared by blending soy protein isolate with different percentage of MMT by melt extrusion technique. The nanocomposites were characterized by using, XRD, TEM, SEM and TGA methods. The XRD studies indicated the absence of diffraction peaks for the bio-nanocomposites. From the TEM studies it was ascertained that the degree of exfoliation increased with increase in MMT content. The morphology of the nanocomposites was ascertained from the SEM studies. The degradation pattern of the nano-composites was evaluated from the TG analysis. The drug delivery system of the nanocmposites was investigated by blending the nanocomposites with ofloxacin at different pH media. The various kinetic parameters were evaluated and the mechanism of drug delivery has been postulated based on the kinetic data.

关键词： Nanocomposites Soy Protein MMT Drug Delivery ofloxacin

PVA Nanofibers Containing ofloxacin-Cyclodextrin Inclusion Complex: Improve Optical Stability of ofloxacin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Organic Polymer Materials 2019年第2期9卷 29-46页

作者： Mohammad Mehrizi Sahar Amiri Seyed Hajir Bahrami Department of Polymer Engineering Science and Research Branch Islamic Azad University Tehran Iran Department of Textile Engineering Amirkabir University of Tehran Tehran Iran

ofloxacin is an antibiotic with a wide range of activity against bacterial infections, but due to the high potential for toxicity when exposed to light, resolving this problem and further stabilizing the drug are among the posed challenges. Inclusion complex formation between α-cyclodextrin (α-CD), ofloxacin (OFL) and polyethylene glycol (PEG) was prepared via two methods to produce nanocontainers with desirable stability. The effect of PEG as compatible solubilizing agent and mixing condition (in ultrasonic bath) were investigated in formation of an inclusion complex between α-CD/OFL. Obtained complexes were examined by FTIR, H-NMR, SEM, EDX and UV which indicated the formation of an inclusion complex between α-CD/OFL, in turn, is a mixture of the cage and channel structures. Differences between 1H-NMR, FTIR and XRD spectra of OFL, CDs and inclusion complex indicated the formation of α-CD/OFL and supramolecular containers in solid phase. These inclusion complexes loaded in PVA-based nanofibers for smart nanofibers with controlled release manner and higher stability of OFL. Obtained nanofiber showed that nanofibers containing CDs/OFL under sonic energy containing higher degree of OFL.

关键词： Supramolecular Containers α-Cyclodextrin ofloxacin Optical Stability Electrospinning