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First Isolation and Characterization of Ochrobactrum anthropi from Pig

Engineering 2020年第1期6卷 49-55页

作者： Shijiang Gu Ruiqing Hou Shengguo Gao Zhe Sun Xiangdong Li Lufeng Zhai Yunyun Jin Qiaoyan Zhu Yonghong Liao Kegong Tian National Research Center for Veterinary Medicine Luoyang 471000China College of Animal Science and Veterinary Medicine Henan Agricultural UniversityZhengzhou 450002China

One Gram-negative Bacillus was isolated from a brain sample of a pig with neurological symptoms.Pathological examination showed meningitis at necropsy. Ochrobactrum anthropi (O. anthropi) was successfully isolated from the brain sample and was confirmed by biochemical reaction results (API 20 NE) and gene sequencing. The strain was highly resistant to b-lactam antibiotics. Mice were experimentally infected with O. anthropi and showed typical meningitis. This is the first report on O. anthropi isolated from a pig, and indicates that O. anthropi may have a broader host spectrum of infection.

关键词： Ochrobactrum anthropi Pig brain

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Identification and characterization of the chromium(Ⅵ) responding protein from a newly isolated Ochrobactrum anthropi CTS-325

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Journal of Environmental Sciences 2009年第12期21卷 1673-1678页

作者： CHENG Yangjian XIE Yongming ZHENG Jing WU Zhaoxian CHEN Zhi MA Xiaoyan LI Bin LIN Zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Fuzhou 350002 China Beijing Deputy Office Bruker Daltonics Inc. Beijing 100081 China Fujian Entry-exit Inspection and Quarantine Bureau Fuzhou 350001 China

A Gram-negative, chromium（Ⅵ） tolerant and reductive strain CTS-325, isolated from a Chinese chromate plant, was identified as Ochrobactrum anthropi based on its biochemical properties and 16S rDNA sequence analysis. It was able to tolerate up to 10 mmol/L Cr（Ⅵ） and completely reduce 1 mmol/L Cr（Ⅵ） to Cr（Ⅲ） within 48 h. When the strain CTS-325 was induced with Cr（Ⅵ）, a protein increased significantly in the whole cell proteins. Liquid chromatography tandem mass spectrometry （LC-MS/MS） analysis revealed that this protein was a superoxide dismutase （SOD） homology. The measured superoxide dismutase activity was 2694 U/mg after three steps of purification. The SOD catalyzes the dismutation of the superoxide anion （O2^←） into hydrogen peroxide and molecular oxygen. This protein is considered to be one of the most important anti-oxidative enzymes for O. anthropi as it allows the bacterium to survive high oxygen stress environments, such as the environment produced during the reduction process of Cr（Ⅵ）.

关键词： chromium LC-MS/MS superoxide dismutase Ochrobactrum anthropi

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Isolation and characterization of a azoxystrobin-degrading bacterial strain Ochrobactrum anthropi SH14

Isolation and characterization of a azoxystrobin-degrading b...

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中国植物病理学会第十二届青年学术研讨会

作者： Shaohua Chen Yinyue Deng Fei He Lianhui Zhang Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR) Department of Biological Sciences National University of Singapore

Continuous use of the strobilurin fungicide azoxystrobin(AZX) has resulted in serious environmental contamination problems.We report here that a novel bacterial strain SH14,which was isolated from wastewater treatment system using an enrichment culture technique,was able to degrade and utilize AZX as the carbon and nitrogen source in minimal medium.Based on the morphology,physio-biochemical characteristics,16 S rDNA gene analysis,and API 20 NE systems,strain SH14 was identified as Ochrobactrum anthropi.Strain SH14 degraded 86.3%of 50 mgL

关键词： azoxystrobin Ochrobactrum anthropi biodegradation kinetics

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一株无色杆菌Ochrobactrum anthropi CGMCC 1649质粒pW240及其衍生质粒的构建及应用

一株无色杆菌Ochrobactrum anthropi CGMCC 1649质粒pW240及其衍...

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第二届中国青年学者微生物遗传学学术研讨会

作者：王利英夏勉温东辉李毅北京未名凯拓农业生物技术有限公司北京大学环境学院环境科学系北京大学生命科学学院生物技术系

目前,分子生物学操作中使用质粒载体相当普遍,但大多数质粒载体来自于大肠杆菌系列,源于土壤、水体和一些污染环境中的环境微生物(如假单胞菌属、产碱杆菌属等)的工程载体并不很多,研究工作还有待深入。

关键词： Ochrobactrum anthropi 质粒荧光标记限制性酶切图谱

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Characterization of Plasmid pWM240 from Ochrobactrum anthropi KT-C024 and its Application as an Abroad Host Range Vector

Characterization of Plasmid pWM240 from Ochrobactrum anthrop...

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第十次全国环境微生物学术研讨会

作者： Wang Liying Qiao Lin Sha Shengde Cao Xiaojin Xia Mian 1,Beijing Wei Ming Kai Tuo Agriculture Biotechnology Co.,Ltd.Beijing,100085 2,Institute of Nuclear and New Energy Technology,Tsinghua University,Beijing,100084

pWM240,a plasmid with the size of 4,277 base-pair,was isolated from Ochrobactrum anthropi KT-C024,and its complete nucleotide sequence was determined.Sequence analysis indicated a putative oriT site and 5 ORFs,including one replication protein (ORF1) and one mobilizing protein (ORF3) in pWM240.Presence of ssDNA intermediate in O.anthropi cells suggested pWM240 replicates via rolling-circle- replication (RCR).pWM241 was constructed by inserting a kanamycin resistance gene into pWM240 and pWM242 was constructed with an addition of green fluorescent protein gene into pWM241 in order to confer pWM240 to screening markers. The mutation of hypothesis mobilizing protein gene (ORF3) disrupted the conjugative function of pWM241,which indicated ORF3 endowed the plasmid with transferable characters,pWM241 was employed successfully to express cyanide-degrading gene cya-1 from Pseudomonas aeruginosa KT-C001,which showed the vector pWM241 has the potential in applying the gene enhanced technology (GET) into wastewater treatment,pWM242 had a broad host range and was stably maintained with antibiotic selection when transformed into Escherichia coli, Pseudomonas aeruginosa,Pseudomonas stutzeri,Paracoccus denitrificans and ochrobactrum sp.The green fluorescent protein was expressed in these species too.

关键词： Ochrobactrum anthropi plasmid rolling-circle- replicating broad host range plasmid gene enhanced technology

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