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Unveiling the emerging role of curcumin to alleviate ochratoxin a-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1638-1656页

作者： Piao Zhao Lin Feng Weidan Jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Lu Zhang Haifeng Mi Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Animal Disease‑Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu 611130SichuanChina Tongwei Co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610041SichuanChina

Background ochratoxin a(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of contamination in aquafeeds and is responsible for severe food *** developmental toxicity of OTA and the potential relieving strategy of natural products remain *** study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in *** A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the control group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur *** treatment consisted of 3 replicates(180 fish)for 60 *** Firstly,we cultured,purified,and identified myoblasts using the tissue block culture *** preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best ***,Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass ***,Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling ***,Cur could downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling *** In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in *** study confirms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

关键词： Curcumin Grass carp Myotoxic ochratoxin a Protein deposition

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Dietary curcumin alleviates intestinal damage induced by ochratoxin a in juvenile grass carp (Ctenopharyngodon idella): Necroptosis and inflammatory responses

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ANIMAL NUTRITION 2024年第3期18卷 119-132页

作者： Zhao, Piao Jiang, Wei-Dan Wu, Pei Liu, Yang Ren, Hong-Mei Jin, Xiao-Wan Feng, Lin Zhou, Xiao-Qiu Sichuan Agr Univ Anim Nutr Inst Chengdu 611130 Sichuan Peoples R China Sichuan Agr Univ Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov Chengdu 611130 Sichuan Peoples R China Minist Agr & Rural Affairs Minist Educ Key Lab Anim Dis Resistance Nutr Key Lab Sichuan Prov Beijing 611130 Sichuan Peoples R China

ochratoxin a (OTA) is one of the most common pollutants in aquatic feed. As a first line of defense, intestinal barriers could be utilized against OTA in order to prevent disorders. Natural product supplementation is one of the most popular strategies to alleviate toxicity induced by mycotoxins, but there is a lack of knowledge about how it functions in the teleost intestine. In this study, 720 juvenile grass carp of about 11 g were selected and four treatment groups (control group, OTA group, curcumin [Cur] group, and OTA + Cur group) were set up to conduct a 60-day growth test. After the test, the growth performance and intestinal health related indexes of grass carp were investigated. The addition of dietary Cur could have the following main results: (1) inhibit absorption and promote efflux transporters mRNA expression, reducing the residuals of OTA, (2) decrease oxidative stress by reducing oxidative damage and enhancing the expression of antioxidant enzymes, (3) promote mitochondrial fusion proteins to inhibit the expression of mitotic proteins and mitochondrial autophagy proteins and enhance mitochondrial function, (4) reduce necroptosis-related gene expression through inhibiting the tumor necrotic factor receptor-interacting protein kinase/mixed lineage kinase domain-like pathway, (5) reduce the expression of pro-inflammatory factors by inhibiting the Toll-like receptor 4/nuclear factor-kappa B signaling pathway to alleviate the intestinal inflammatory response. In summary, the results suggested that Cur could alleviate OTA-induced intestinal damage by enhancing antioxidant capacity and mitochondrial function as well as reducing necroptosis and inflammation in the grass carp intestine. This study provided a theoretical basis and production implications for dietary Cur that could improve growth performance and alleviate the intestinal damage induced by OTA in fish. (c) 2024 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co.

关键词： Curcumin ochratoxin a Grass carp growth performance Mid-intestine mitochondria function Necroptosis NF-KAPPA-B GROWTH-PERFORMANCE FISH METABOLISM TOXICITY EXPOSURE IMMUNITY MODEL

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ochratoxin a induces abnormal tryptophan metabolism in the intestine and liver to activate AMPK signaling pathway

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2674-2685页

作者： Weiqing Ma Yang Fu Shanshan Zhu Daiyang Xia Shuangshuang Zhai Deqin Xiao Yongwen Zhu Michel Dione Lukuyu Ben Lin Yang Wence Wang Guangdong Provincial Key Laboratory of Animal Nutrition and Regulation College of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China. School of Marine Sciences Sun Yat-Sen Universityand Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai 519082China. College of Animal Science YangtzeUniversityJingzhou 434025China.4 College of Mathematics and Informatics South China Agricultural UniversityGuangzhou 510642China. Int Livestock Res Inst 24265 DakarSenegal. Int Livestock Res Inst Nairobi 00100Kenya

Background ochratoxin a(OTA)is a mycotoxin widely present in raw food and feed materials and is mainly pro-duced by Aspergillus ochraceus and Penicillium *** previous study showed that OTA principally induces liver inflammation by causing intestinal flora disorder,especially Bacteroides plebeius(***)***,whether OTA or *** alteration leads to abnormal tryptophan-related metabolism in the intestine and liver is largely *** study aimed to elucidate the metabolic changes in the intestine and liver induced by OTA and the tryptophan-related metabolic pathway in the *** and methods A total of 30 healthy 1-day-old male Cherry Valley ducks were randomly divided into 2 *** control group was given 0.1 mol/L NaHCO3 solution,and the OTA group was given 235μg/kg body weight OTA for 14 consecutive *** metabolites were determined by intestinal chyme metabolomics and liver tryptophan-targeted ***-related signaling pathway factors were analyzed by Western blot-ting and mRNA *** Metabolomic analysis of the intestinal chyme showed that OTA treatment resulted in a decrease in intesti-nal nicotinuric acid levels,the downstream product of tryptophan metabolism,which were significantly negatively correlated with *** *** contrast,OTA induced a significant increase in indole-3-acetamide levels,which were positively correlated with *** ***,OTA decreased the levels of ATP,NAD+and dipeptidase in the *** tryptophan metabolomics analysis showed that OTA inhibited the kynurenine metabolic pathway and reduced the levels of kynurenine,anthranilic acid and nicotinic ***,OTA increased the phosphorylation of AMPK protein and decreased the phosphorylation of mTOR *** OTA decreased the level of nicotinuric acid in the intestinal tract,which was negatively correlated with *** *** abnormal metabolism of try

关键词： AMPK Metabolomics ochratoxin a Tryptophan metabolism

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ochratoxin a:Carryover from animal feed into livestock and the mitigation strategies

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Animal Nutrition 2021年第1期7卷 56-63页

作者： Abirami Ramu Ganesan Balamuralikrishnan Balasubramanian Sungkwon Park Rajesh Jha Ines Andretta Archibold G.Bakare In Ho Kim Department of Food Science and Home Economics School of Applied SciencesCollege of EngineeringScience and TechnologyFiji National UniversitySuvaFiji Islands Department of Food Science and Biotechnology College of Life ScienceSejong UniversitySeoulSouth Korea Department of Human Nutrition Food and Animal SciencesCollege of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaHonoluluHIUSA Universidade Federal do Rio Grande do Sul Faculdade de AgronomiaDepartment of Animal SciencePorto AlegreRio Grande do SulBrazil Department of Animal Science College of AgricultureFisheries&ForestryFiji National UniversityFiji Islands Department of Animal Resource and Science Dankook UniversityCheonanSouth Korea

This review aims to highlight the effects of ochratoxin a(OTA)in the feed of meat-producing *** accumulation of OTA in feed and its distribution in various farm animals were compared and ***,the oral administration of OTA-contaminated feed and the predisposition in an animal's vital organ were critically examined in this *** collated reports show that OTA directly associated with endemic nephropathy and its high concentration leads to degeneration of liver cells,and necrosis of intestinal and lymphoid *** present,limited reports are available in the recent liter-ature on the problems and consequences of OTA in ***,this review focused on the OTA carryover from feed to farm animals and the interaction of its secondary metabolites on their biochemical ***,this report provides greater insights into animal health related to OTA residues in meat and meat *** article also explores mitigation strategies that can be used to prevent the carryover effects of OTA in livestock feeds and the effects in the food chain.

关键词： ochratoxin a Feed Livestock Meat Mitigation strategy

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Characterization and Comparison of ochratoxin a-Ovalbumin(OTA-OVA) Conjugation by Three Methods

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Journal of Integrative Agriculture 2014年第5期13卷 1130-1136页

作者： WANG Xi-chun BAO Ming WU Jin-jie LUO Ying MA Liang-you WANG Ying ZHANG Ai-hua HE Cheng-hua ZHANG Hai-bin College of Animal Science and Technology Anhui Agricultural University Aquatic Technology Promotion Station of Anhui Province College of Veterinary Medicine Nanjing Agricultural University

In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten （ochratoxin a, OTA） was coupled to ovalbumin （OVA） by an active ester reaction. To develop a technique for detecting the conjugation, the hapten-protein conjugate （OTA-OVA） was characterized thoroughly by immunoarray technology, ultraviolet （UV） spectroscopy and matrix-assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF-MS）, respectively. The molecular weight of OTA-OVA was 50 350.141 Da, and the molecular weight of OVA was 44 887.506 Da, which were determined by MALDI-TOF-MS, respectively. In OTA-OVA, the molecular coupling ratio was 13：1 by MALDI-TOF-MS while the molecular coupling ratio was 10：1 by UV. In this experiment, UV and MALDI-TOF-MS were selected as the efficient methods to evaluate the cross-linking effect and calculate the molecular coupling ratio.

关键词： ochratoxin a conjugated antigen cartier protein coupling ratio

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A Label-free Fluorescent Aptasensor for Turn-on Monitoring ochratoxin a Based on AIE-active Probe and Graphene Oxide

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Chemical Research in Chinese Universities 2018年第3期34卷 363-368页

作者： MA Lian XU Bin LIU Leijing TIAN Wenjing State Key Laboratory of Supramolecular Structure and Materials Jilin University Changehun 130012 P. R. China

A label-free fluorescent aptasensor for specific and ultrasensitive monitoring ochratoxin a（OTA） was developed using the specific aptamer of OTA（OSA） as recognition dement, an aggregation-induced emission（AIE） molecule（a 9,10-distyrylanthracene with two ammonium groups, DSAI） as a fluorescent probe, and graphene oxide（GO） as a quencher. In the absence of OTA, the AIE probe DSAI and OSA complex（DSAI/OSA） is adsorbed on the GO surface, and the fluorescence of DSAI will be quenched efficiently via the fluorescence resonance energy transfer（FRET） from DSAI to GO. Upon the OTA addition, a more stable complex（OSA-OTA） is formed and released from GO. Meanwhile, DSAI and OSA-OTA can form a new complex（DSAI/OSA-OTA）, then the fluorescent signal of DSAI recovers gradually. Therefore, by introducing GO and DSAI, the fluorescence signal of DSAI can be easily turned from ＂off＂ to ＂on＂ after the addition of OTA, and the ultrasensitive detection of OTA by monitoring the change of the fluorescence signal of DSAI can be readily realized. The detection limit of the assay can reach 0.324 nmol/L with a linear detection range of 10-200 nmol/L. And the aptasensor exhibits high selectivity for OTA against other analogues. Moreover, it has been successfully applied for the detection of OTA in red wine samples.

关键词： Label-free Aptasensor Aggregation-induced emission(AIE)-active probe Graphene oxide ochratoxin a

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Aptamer based detection and separation platforms for ochratoxin a:A systematic review

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BIOCELL 2022年第12期46卷 2537-2557页

作者： RAHELEH TORABI ABBAS ALI REZVANIPOUR HADI ESMAEILI GOUVARCHINGHALEH REZA RANJBAR MOHAMMAD HEIAT Laboratory of Bioanalysis Institute of Biochemistry&BiophysicsUniversity of TehranTehranIran Baqiyatallah Research Center for Gastroenterology and Liver Diseases(BRCGL) Baqiyatallah University of Medical SciencesTehranIran Applied Virology Research Center Baqiyatallah University of Medical SciencesTehranIran Molecular Biology Research Center Systems Biology and Poisonings InstituteBaqiyatallah University of Medical SciencesTehranIran

ochratoxin a(OTA),one of the most dangerous mycotoxins for human health,has been subjected to numerous studies for separation and detection in minimal *** as novel recognition elements have been employed to fabricate ultrasensitive biosensors for the detection of OTA and designing delicate analytical *** review attempted to comprehensively examine all reported aptamer-based detection and separation platforms for *** most relevant databases were considered to discover all specific aptamers for dealing with ***-based detection and separation devices specified for OTA were searched for,analyzed,discussed,and classified based on their *** optical aptasensors have gathered a higher interest than electrochemical aptasensors,which can achieve a lower limit of ***,some extraction platforms based on these aptamers were also ***,aptamer-based devices seem to have some challenges in their application.

关键词： Aptamer Aptasensors ochratoxin a Mycotoxin Biosensor

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Determnation of ochratoxin a in grain by monoclonal antibody－based enzyme－linked immunosorbent assay

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Journal of Environmental Sciences 1994年第1期6卷 116-122页

作者： Yang Chuanhe Luo Xueyun Liu Chang Li Wenyan Li Yiepeng Zhao Danyu Ji RongInstitute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China

The simple rapid and sensitive enzyme-linked immunosorbent assay (ELISA) methods, di-rect and indirect ELISA, for quantitation of ochratoxin a in cereal had been developed by theutilization of monoclonal antibody on immunomicroplate. Direct FLIAS was found to be less timeconsuming than indirect ELISA. For direct FLISA, recovery of 1 -500 ppb OA added to wheat was78.9-100.0% and rice was 88.9- 120.0%. For indirect ***, recovery of 1-500 ppb OA addedto wheat was 79.0- 110.0% and rice was 82.0 120.0%. The minimal detection level for OA was Ippb. Analyses of 31 samples that caused humanintoxicant for OA showed that the ELISA resultsagreed wtll with those obtained by thin-layer chromatogrdphy.

关键词： enzyme-linked immunosorbent assay (ELISA) ochratoxin a monoclonal antibody cereal.

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Molecularly imprinted polymer based on upconversion nanoparticles for highly selective and sensitive determination of ochratoxin a

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Journal of Central South University 2019年第3期26卷 515-523页

作者： YAN Zhen FANG Guo-zhen State Key Laboratory of Food Nutrition and Safety Tianjin University of Science and Technology

A novel molecularly imprinted polymer (MIP) based on upconversion nanoparticles (UCNPs) was successfully synthesized for determination of ochratoxin a (OTA). The MIP was developed on the silica-coated UCNPs using N-(1-hydroxy-2-naphthoyl amido)-(L)-phenylalanine (HNA-Phe) as the alternative template. The final composite combined the advantages of the high selectivity of MIP with the high fluorescence intensity of UCNPs which was selective and sensitive to OTA. Under the optimal condition, the fluorescence intensity of UCNPs@SiO2@MIP decreases linearly when the concentration of OTA increases from 0.05 to 1.0 mg/L. The detection limit of OTA with the method was 0.031 mg/L. At three spiked concentration levels (50, 100 and 200 μg/kg), the recovery ranges of OTA in corn, rice and feed are 88.0%–91.6%, 80.2%–91.6% and 89.2%–90.4%, respectively.

关键词： molecularly imprinted polymer upconversion nanoparticles fluorescent sensing ochratoxin a

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Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin a and zearalenone in corn, wheat, and feed samples

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第11期19卷 871-883页

作者： Xian ZHANG Ke HE Yun FANG Tong CAO Narayan PAUDYAL Xiao-feng ZHANG Hou-hui SONG Xiao-liang LI Wei-huan FANG China-Australian Joint Laboratory for Animal Health Big Data Analytics Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet TechnologyCollege of Animal Science and TechnologyZhejiang A&F UniversityLin'an 311300China Zhejiang University Institute of Preventive Veterinary Medicine Zhejiang Provincial Key Laboratory of Preventive Veterinary MedicineHangzhou 310058China Zhejiang Academy of Science and Technology for Inspection and Quarantine Hangzhou 310012China

A one-step dual flow immunochromatographic assay （DICGA）, based on a competitive format, was de- veloped for simultaneous quantification of ochratoxin a （OTA） and zearalenone （ZEN） in corn, wheat, and feed sam- ples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53-12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06-39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry （LC-MS/MS） and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.

关键词： Immunochromatographic assay Gold nanoparticles ochratoxin a Zearalenone Quantification

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