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Domestication of marine microalga Nannochloropsis oceanica to freshwater medium and the physiological responses

Journal of Oceanology and Limnology 2019年第4期37卷 1353-1362页

作者： GUO Li LIANG Sijie ZHANG Zhongyi LIU Hang WANG Songwen YANG Guanpin College of Marine Life Sciences Ocean University of China Qingdao 266003 China Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China Qingdao 266003 China Institutes of Evolution and Marine Biodiversity Ocean University of China Qingdao 266003 China College of Agriculture and Resources and Environment Tianjin Agricultural University Tianjin 300384 China

Marine microalga Nannochloropsis oceanica LAMB0001 were domesticated (~730 generations,~two days each) to adapt freshwater BG11 medium. A number of freshwater medium adapted colony-derived strains were obtained. The strains were verified phylogenetically to be N. oceanica LAMB0001 based on the 18S ribosomal RNA gene. Freshwater-medium adapted strain (FA1) grew faster in the BG11 medium prepared with freshwater than wild-type N. oceanica grew in f/2 medium prepared with seawater. We assumed that (1) the expression patterns of the genes that expressed differentially between FA1 and the wild-type N. oceanica exposing to the BG11 medium (WT-F) have been reprogrammed;(2) the physiological processes in which these genes involved have been modified;and (3) a Gene Ontology (GO) term or a KEGG pathway enriched by DEGs between FA1 and WT-F has been up- or down-regulated if it was enriched simultaneously by up- or down-regulated DEGs between FA1 and WT-F, respectively. Under these assumptions, we found that FA1 reprogrammed the expression patterns of a set of genes that involved in cell adhesion, membrane and membrane integrity, material transportation, cell movement, and cellular signaling network. These changes in cellular functions and metabolic pathways indicate that the microalga modified its gene expression pattern in a wide function range and at a high regulation rank in order to adapt to the freshwater medium. It is feasible to domesticate marine microalgae to a freshwater habitat, which may aid to modify their cultivation performances.

关键词： Nannochloropsis oceanica domestication acclimation adaptation genetic variation

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Transcriptomic Response of Nannochloropsis oceanica to Benzo[a]pyrene

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Journal of Ocean University of China 2019年第3期18卷 680-686页

作者： ZHANG Zhongyi GUO Li LIU Hang LIANG Sijie YANG Guanpin Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China Qingdao 266003 China College of Marine Life Sciences Ocean University of China Qingdao 266003 China Institutes of Evolution and Marine Biodiversity Ocean University of China Qingdao 266003 China

In this study, the RNA sequencing was used to describe the response of Nannochloropsis oceanica, a marine microalga, to benzo[a]pyrene(BaP), a polycyclic aromatic hydrocarbon, in order to elucidate the metabolic pathways(or processes) involved in microalgal response to this stubborn pollutant. N. oceanica was exposed to BaP at a concentration of 90 μg L^-1 for 72 h, and its transcriptome was sequenced through the Illumina HiSeq^TM 2500 platform. This concentration of BaP was selected as it is the lowest for modeling the most appropriate growth inhibition of N. oceanica for transcriptomic analysis. We found that N. oceanica responds to BaP through degrading proteins and repairing DNA damaged by BaP. In addition, superoxide dismutase(SOD) strengthened its performance by increasing its transcript abundance. The physiological mechanism underlining the response of N. oceanica to BaP as revealed by transcriptomic analysis was consistent with the biochemical insights documented previously.

关键词： Nannochloropsis oceanica benzo[a]pyrene transcriptome physiological response

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Trace metals in Posidonia oceanicain a coastal area of the Ionian Sea (Calabria, Italy)

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Open Journal of Ecology 2013年第2期3卷 102-108页

作者： Radiana Cozza Adele Iaquinta Davide Cozza Luisa Ruffolo Dipartimento di Biologia Ecologia e Scienze della Terra Università della Calabria Arcavacata di Rende Cosenza Italy

As, Cd, Cu and Pb concentrations were measured in Posidonia oceanica sampled from meadows located in two sites along the calabrian coast (Ionian Sea, South-eastern Mediterranean). By dating the scales and the rhizome using retrospective procedures (lepidochronology), a time series over a period of nine years (1995-2004) was analysed. Throughout the whole lepidochronological period, the arsenic content in the scales was tenfold higher than that measured in the rhizome with a peak in the lepidochronological year 1996-1997 and a general decrease in the years after. Pb concentration also showed a tendency to decrease with time, whereas the Cd and Cu concentration were increasing. A comparison of the metal level in dead sheaths (scales) and living tissue (leaves) was also performed. The detailed distribution of the trace metals along the leaf axis and in the leaves at different developmental stages (adult, intermediate and juvenile) showed some differences in the metal content. The results also indicate that the arsenic content measured in the leaf blade reflects the high As content measured at the superficial sediments at one of the two sampled sites, demonstrating, for the first time, that P. oceanica would be a good indicator for this element.

关键词： Biomonitoring Heavy Metals Ionian Sea Lepidochronology Posidonia oceanica

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Comparative analysis of bed density, total phenol content and protein expression pattern in Posidonia oceanica(L.) Delile

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Open Journal of Ecology 2013年第6期3卷 438-444页

作者： Alice Rotini Letizia Anello Maria Di Bernardo Agata Giallongo Luigi Valiante Luciana Migliore Dipartimento di Biologia Università Tor Vergata Rome Italy ECON Sistemi Avanzati per l’Ambiente s.r.l. Naples Italy Istituto di Biomedicina e Immunologia Molecolare Consiglio Nazionale delle Ricerche Palermo Italy

Posidonia oceanicameadows are experiencing a progressive decline, and monitoring their status is crucial for the maintenance of theseecosystems. We performed a comparativeanalysis of bed density, total phenol content and protein expression pattern to assess the conservation status ofPosidoniaplants from the S. Marinella (Rome, Italy) meadow. The total phenol content was inversely related to maximum beddensity, confirming the relationship betweenhigh phenol content and stressful conditions. In addition, protein expression pattern profilesshowed that the number of differentially expressed proteins was dramatically reduced in the latest years compared to previous analyses. Our results support the usefulness of integrating solid descriptors, such as phenol content, with novel biochemical/molecular approaches in the monitoring of meadows.

关键词： Posidonia oceanica Rhizome Seagrass Monitoring Total Phenols 2-D Electrophoresis (2-DE) Bed Density Protein Expression Pattern

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Assessment of Posidonia oceanica(L.) Delile conservation status by standard and putative approaches: the case study of Santa Marinella meadow (Italy, W Mediterranean)

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Open Journal of Ecology 2011年第2期1卷 48-56页

作者： Alice Rotini Carla Micheli Luigi Valiante Luciana Migliore 不详

The conservation status of the Posidonia oceanica meadow at Santa Marinella (Rome) was evaluated through both standard (bed density, leaf biometry, "A" coefficient, Leaf Area Index, rhizome production) and biochemical/genetic approaches (total phenol content and Random Amplified Polymorphic DNA marker). The bio-chemical/genetic results are in agreement with those obtained by standard approaches. The bed under study was ranked as a disturbed one, due to its low density, and high heterogeneity in leaf biometry, LAI values, "A" coefficient and primary production. This low quality ranking is confirmed by both mean phenol content in plants, quite high and scattered, and by the low genetic variability in the meadow, with a very high similarity of specimen at a local scale. Hence, these two putative approaches clearly identify the endangered conservation status of the meadow. They link plant biodiversity and ecophysiology to ecosystem 'health'. Furthermore, they are repeatable and standardizable and could be usefully introduced in meadows monitoring to check environmental quality.

关键词： Posidonia oceanica Genetic Variability Enviironmental Indicator RAPD Seagrass Monitoring Total Phenols

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Effect of Nitrogen Source on Biomass and Lipid Production of a Marine Microalga, Nannochloropsis oceanicaIMET1

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Green and Sustainable Chemistry 2015年第2期5卷 101-106页

作者： Yongxue Chi Feng Chen Yasuyuki Takiguchi Department of Life and Environmental Sciences Graduate School of Engineering Chiba Institute of Technology Chiba Japan Institute of Marine and Environmental Technology University of Maryland Center for Environmental Science Baltimore MD USA

The effects of the nitrogen sources sodium nitrate (NaNO3) and urea (CH4N2O) on growth, lipid production, and fatty acid composition of Nannochloropsis oceanica IMET1 were investigated. Nitrogen source affected cell density, dry cell weight, and lipid production. Cells grown in the nitrate medium increased dry cell weight and lipid weight in comparison with cells grown in the urea medium. The composition of fatty acids varied with nitrogen sources. IMET1cultured in the nitrate medium mainly contained C18:2 (14.9%) and C16:0 (6.3%) fatty acids, while IMET1 in the urea medium mainly contained C22:0 (33.1%), C18:3 (8.6%), and C16:0 (6.8%). This study demonstrates that nitrogen source can strongly influence lipid production and composition of N. oceanica IMET1.

关键词： Nannochloropsis oceanica Nitrogen Source Lipid Production Growth Performance Fatty Acid Composition

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Diet of fish populations in posidonia oceanica meadows off the Island of Ischia (Gulf of Naples, Italy):

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Natural Science 2010年第11期2卷 1274-1286页

作者： Valerio Zupo Dorothea Stübing 不详

The gut contents of fish in three Posidonia oceanica meadows off the island of Ischia (Bay of Naples, Italy) were investigated. A total of 926 individual fish belonging to 28 species was sampled by bottom trawl in the leaf canopy. Labridae, Pomacentridae, Scorpaenidae, and Serranidae were the best represented families (41%, 38%, 8% and 6% of the total number of individuals, respectively). Of the 94 taxa detected in the gut contents, 42 were identified to the species level. The most common food items were decapod crustaceans (15% of the gut contents, on average), copepods (13%), amphipods (14%), brown fragments of P. oceanica (6%), and ostracods (6%). The most abundant species of labridae, Symphodus ocellatus and S. rostratus, showed a broad spectrum of prey. This generalist feeding may positively influence their numerical abundance. Seasonal variations in the diets of fish, also at prey-species level, were demonstrated. The fish taxon plays essentially a macro-carnivore trophic role. In the investigated seagrass meadows the main trophic fluxes start from plant detritus, macrophyta, and microphyta (as primary producers) towards crustacean decapods, copepods, ostracods, and gammarid amphipods (as secondary producers) to fish. A low recycling rate (4%) within the fish community was observed. Larger fish predators (e.g., Sparidae), swimming over the leaf canopy, are the main exporters to adjacent coastal systems.

关键词： Fish Food Webs Spatial Variability Posidonia oceanica Seagrass Seasonality

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Epiphytic Diatom Communities on Sub-Fossil Leaves of Posidonia oceanicaDelile in the Graeco-Roman Harbor of Neapolis: A Tool to Explore the Past

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American Journal of Plant Sciences 2014年第5期5卷 549-553页

作者： Paola Cennamo Paolo Caputo Mario De Stefano Elda Russo Ermolli Maria Rosaria Barone Lumaga Dipartimento di Biologia Orto Botanico Università di Napoli “Federico II” Naples Italy Dipartimento di Scienze della Terra dell'Ambiente e delle Risorse (DISTAR) Università di Napoli Federico II Naples Italy. Département de Préhistoire Muséum National d’Histoire Naturelle Centre National de la Recherche Scientifique Paris France Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche Seconda Università di Napoli Caserta Italy Facoltà di Lettere Università degli Studi Suor Orsola Benincasa Naples Italy

The Graeco-Roman harbor of Neapolis (Naples, Italy), chronologically constrained between the late 4th century BC and the 6th century AD, offers a unique sight on relationship between past and present into the history of the marine biodiversity. In fact, the digs expose fossil leaf of Posidonia oceanica with epiphytic communities of microorganisms. Posidonia oceanica is a seagrass endemic to theMediterraneanthat forms large meadows whose remains can persist in the sediment for thousands of years. In this communication, we report results of analyses carried out using both molecular and morphological techniques on sub-fossil leaves of P. oceanica and their associated epiphytic communities.

关键词： Posidonia oceanica Epiphytic Communities Ancient DNA

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Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae

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Molecular Plant 2019年第4期12卷 474-488页

作者： Yi Xin Chen Shen Yiting She Hong Chen Cong Wang Li Wei Kangsup Yoon Danxiang Han Qiang Hu Jian Xu Single-Cell Center CAS Key Laboratory of Biofuels and Shandong Laboratory of Energy Genetics Qingdao Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences Qingdao Shandong 266101 China Center for Microalgal Biotechnology and Biofuels Institute of Hydrobiology Chinese Academy of Sciences Wuhan Hubei 430072 China Core Laboratory Qingdao Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences Qingdao Shandong 266101 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao Shandong 266237 China University of Chinese Academy of Sciences Beijing 100049 China

The composition of polyun saturated fatty acids (PUFAs) in triacylglycerols (TAGs) is key to health ben efits and for oil applications, yet the underlying genetic mechanism remains poorly understood. In this study, by in si Iico, ex vivo, and in vivo profili ng of type-2 diacylglycerol acyltransferases (DGAT2s) in Nannochloropsis oceanica we revealed two novel PUFA-preferring enzymes that discriminate individual PUFA species in TAG assembly, with NoDGAT2J for linoleic acid (LA) and NoDGAT2K for eicosapentaenoic acid (EPA). The LA and EPA composition of TAG molecules is mediated in vivo via the functional partitioning between NoDGAT2J and 2K, both of which are localized in the chloroplast envelope. By modulating transcript abundance of the DGAT2s, an N. oceanica strain bank was created, where proportions of LA and EPA in TAG vary by 18.7-fold (between 0.21% and 3.92% dry weight) and 34.7-fold (between 0.09% and 3.12% dry weight), respective!y. These findings lay the foundation for producing designer TAG molecules with tailored health benefits or for biofuel applications in industrial microalgae and higher-plant crops.

关键词： microalgae polyunsaturated fatty acids diacylglycerol acyltransferase Nannochloropsis oceanica

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