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Inhibition of obtusifolin on retinal pigment epithelial cell growth under hypoxia

International Journal of Ophthalmology(English edition) 2019年第10期12卷 1539-1547页

作者： Li-Fei Wang Zhong-Yang Yan Ya-Lin Li Yan-Hui Wang Sheng-Juan Zhang Xin Jia Lu Lu Yan-Xia Shang Xin Wang Yun-Huan Li Shan-Yu Li Fundus Surgery Ward Hebei Provincial Eye HospitalXingtai054001Hebei ProvinceChina Diabetic Eye Disease Ward Hebei Provincial Eye HospitalXingtai054001Hebei ProvinceChina Corneal Disease Ward Hebei Provincial Eye HospitalXingtai054001Hebei ProvinceChina

AIM: To explore the effect of obtusifolin on retinal pigment epithelial cell growth under ***: In vitro chemical hypoxia model of ARPE-19 cells was established using cobalt chloride(CoCl2). Cell viability was tested by cell counting kit-8(CCK-8) assay. Western blot and real-time quantitative polymerase chain reaction were applied to detect proteins and mRNAs respectively. Flow cytometry was used to examine the cell cycle. Secretion of vascular endothelial growth factor(VEGF) was tested by using enzyme linked immunosorbent assay(ELISA).RESULTS: Under the chemical hypoxia model established by CoCl2, hypoxia inducible factor-1α(HIF-1α) mRNA and protein levels was up-regulated. Cell viability was increased and the proportion of S phase was higher. obtusifolin could reduce cell viability under hypoxic conditions and arrest cells in G1 phase. obtusifolin reduced the expression of Cyclin D1 and proliferating cell nuclear antigen(PCNA) in the hypoxic environment and increased the expression of p53 and p21. The levels of VEGF, VEGFR2 and eNOS proteins and mRNA were significantly increased under hypoxia while obtusifolin inhibited the ***: obtusifolin can inhibit cell growth under hypoxic conditions and down-regulate HIF-1/VEGF/eNOS secretions in ARPE-19 cells.

关键词： retinal pigment epithelial cells obtusifolin vascular endothelial growth factor hypoxia

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