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DNA Barcoding:An Effective Tool for Species Identification of Lepidopteran oak pests

Animal Husbandry and Feed Science 2018年第2期10卷 103-108页

作者： Yang Ruisheng Zhang Junyi Chen Yubo Wang Yong Jiang Yiren Shi Shenglin Qin Li College of Bioscience and Biotechnology Shenyang Agricultural UnirersityLiaoning Engineering and Techuology Research Center for Insect Resource

[Objective] The paper was to improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests.[Method] DNA barcoding technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit Ⅰ（COⅠ） as the standard *** barcoding technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from oak.[Result] The DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran *** were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,*** obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest *** them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects（No.1-20） had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples（No.21-22） had high difference degree（8.6%） with homologous sequences.[Conclusion] The established DNA barcoding technique is an effeetive tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.

关键词： DNA bareode oak pest Lepidopteran insects Molecular identification Cytochrome CO Ⅰ

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DNA barcode:an effective tool for species identification of lepidopteran oak pests

DNA barcode:an effective tool for species identification of ...

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中国蚕学会2021年学术年会

作者： Ni Mingyang Gu Yujian Jiang Yiren Shi Shenglin Yang Ruisheng Department of Sericulture College of Bioscience and BiotechnologyShenyang Agricultural University

To improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests,DNA barcode technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit I（CO I） as the standard *** technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from *** DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran *** were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,*** obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest *** them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples had high difference degree（8.6%） with homologous *** established DNA barcoding technique is an effective tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.

关键词： DNA barcode oak pest Lepidopteran insects Molecular identification Cytochrome C oxidase subunit I

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