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Microbiological assessment of indoor air of a teaching hospital in Nigeria

Asian Pacific Journal of Tropical Biomedicine 2012年第6期2卷 465-468页

作者： Awosika SA Olajubu FA Amusa NA Department of Microbiology Olabisi Onabanjo University Department of Medical Microbiology & Parasitology Olabisi Onabanjo University Teaching Hospital Department of Plant Science & Applied Zoology Olabisi Onabanjo University

Objective:To investigate the quality of indoor air of different wards and units of Olabisi Onabanjo University Teaching Hospital, Sagamu, to ascertain their contribution to infection rate in the hospital.Methods:The microbial quality of indoor air of nine wards/units of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria was conducted. Sedimentation technique using open Petri-dishes containing different culture media was employed and samplings were done twice daily, one in the morning shortly after cleaning and before influx of people/patients into the wards/units and the other in the evening when a lot of activities would have taken place in these wards. Isolates were identified according to standard methods.Results:Results showed that there was a statistically significant difference(氈2= 6.0167) in the bacteria population of the different sampling time whereas it was not so for fungi population(氈2= 0.2857). Male medical ward(MMW) and male surgical general(MSG) recorded the highest bacterial and fungal growth while the operating theatre(OT) was almost free of microbial burden. The bacteria isolates were Staphylococcus aureus, Klebsiellasp., Bacillus cereus, Bacillus subtilis, Streptococcus pyogenes andSerratia marscenceswhile the fungi isolates includedAspergillus flavus, Penicilliumsp.,Fusariumsp.,Candida albicansandAlternariasp.Staphylococcus aureuswas the predominantly isolated bacterium whilePenicilliumsp. was the most isolated fungus.Conclusions:Though most of the microbial isolates were potential and or opportunistic pathogens, there was no correlation between the isolates in this study and the surveillance report of nosocomial infection during the period of study, hence the contribution of the indoor air cannot be established. From the reduction noticed in the morning samples, stringent measures such as proper disinfection and regular cleaning, restriction of patient relatives' movement in and out of the wards/units need to be enforced so as to improve the quality of indoor air of our hospital w

关键词： Indoor air oouth Open-plate technique Nosocomial infections Staphylococcus aureus Microbiological assessment Microbial quality Bacterial isolate Fungal isolate Opportunistic pathogen Nosocomial infection Infection rate Microbial isolate Penicilliumsp Sedimentation technique

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Seminal Fluid Characteristics of Men Attending Infertility Clinic of a Teaching Hospital

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Open Journal of Medical Microbiology 2013年第1期3卷 1-4页

作者： Festus Abiose Olajubu Deji-Agboola Mope Olubunmi Adetokunbo Osinupebi Olusoji Edward Jagun Department of Medical Microbiology & Parasitology Obafemi Awolowo College of Health Sciences Olabisi Onabanjo University Sagamu Nigeria Department of Medical Microbiology & Parasitology Obafemi Awolowo College of Health SciencesOlabisi Onabanjo University Sagamu Nigeria Department of Medical Microbiology & Parasitology Olabisi Onabanjo University Teaching Hospital Sagamu Nigeria Department of Obstetrics and Gynaecology College of Health Sciences Olabisi Onabanjo University Sagamu Nigeria

The semen quality of 479 men in infertile marriages, who attended the infertility clinic of oouth, Sagamu between May 2004 and April 2009 were analyzed. The semen samples were physically examined, microscopically analyzed and aerobically cultured. The result showed that out of 51.5% oligospermic cases recorded, 39.8% could be classified severe. Cryptozoospermia were diagnosed in 72 (15%) cases. Bacteriospermia and Necrozoospermia were found in 149 and 6 cases respectively. The predominant organism isolated was Staphylococcus aureus (50.32%) while Neisseria gonorrhoea was isolated in only 2 cases. Highest infection of 62.4% was noticed among the group with 1% - 19% motility. From the studied population, 8.8% were azoospermic. The commonest morphological defects were pin and ragged heads (48%). It is therefore obvious that the assessment of semen quality remains one of the important steps in infertility interventions.

关键词： Sperm Concentration oouth Infertile Marriages Motility Bacteriospermia

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