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Effect of all-trans retinoic acid on growth of xenograft tumor and its metastasis in nude mice

Chinese Medical Journal 2000年第4期 57-61页

作者：陈玉强吴乔陈正明陈福苏文金

Objective To study the effect of all trans retinoic acid on growth of xenograft tumor and its metastasis in nude mice Methods Human gastric cancer BGC 823 and MKN 45 cells were inoculated into spleen subcapsule of nude mice, respectively The nude mice were subsequently administered with all trans retinoic acid every other day Food consuming and body weight of nude mice were measured weekly Six weeks later, the nude mice were killed Xenograft tumors in spleen and metastatic tumors in liver were pathologically examined Microvessel density in the tumors was detected immunohistochemically, and serum carcinoembryonic antigen was measured by radioimmunoassay Results After the nude mice were fed with all trans retinoic acid, the growth of splenic tumor and its liver metastasis were inhibited and the metastatic rates decreased by 50% (BGC 823) and 33 3% (MKN 45), respectively The microvessel density in splenic and hepatic tumors reduced by 28 58% and 35 47% (BGC 823), 19 45% and 14 52% (MKN 45), respectively The concentration of carcinoembryonic antigen decreased by 50 24% (BGC 823) and 48 10% (MKN 45) Conclusion All trans retinoic acid may effectively inhibit the growth of xenograft tumor in spleen and its metastasis to liver in nude mice, which can be corroborated by the decrease of carcinoembryonic antigen and microvessel density

关键词： all trans retinoic acid xenograft tumor metastasis nude mouse gastric cancer cell

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THE　COMPARISON　STUDY　OF　ESTABLISHING　ANIMAL　MODEL　OF　HUMAN　UTERINE　CANCERUSING　MATRIGEL

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Chinese Journal of Cancer Research 1995年第3期7卷 168-171页

作者：邓小虹 Department of Oncology Beijing Obstet & Gynecol Hospital Beijing China Department of Obstetrics & Gynecology Nihon University School of Medicine Tokyo Japan

The human endometrial adenocarcinoma cell liueIskikawa cells suspended either in Matrigel or MEM buffer medium were injected subcutaneously to nude mice (N=10 mice/group);and the fresh human cervical cancer tissues obtained directly from the surgery were coated by Matrigel and transplanted into nude mouse. The purpose of these studies were to see if Matrigel could enhance the human uteriue cancers' proliferation and differentiation in *** results showed that when cancer cells or tissues implanted into nude mice in Matrigel, the xenografts could turn out earlier, proliferate faster and kept the original differentiation *** is suggested that Matrigel is an ideal agent in establishing animal models of human uterine cancer.

关键词： Matrigel Endometrium cancer cell Implantation nude mouse Cervical cancer.

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In vivo comparison of transduction efficiency with recombinant adenovirus-mediated p53 in a human colon cancer mouse model by different delivery routes

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The Chinese-German Journal of Clinical Oncology 2008年第12期7卷 704-708页

作者： Qi Xie Biling Liang Jing Zhang Qihua Yang Xiongfei Gu Jing Xu Mingwang Chen Department of Radiology Nansha Center Hospital Guangzhou Municipal First People＇s Hospital Guangzhou Medical College Guangzhou 510180 China Department of Radiology the Second Affiliated Hospital Sun Yat-sen University Guangzhou 510275 China Department of Pathology Guangzhou Municipal First People＇s Hospital Guangzhou Medical College Guangzhou 510180 China Department of Biochemistry Medical School of Sun Yat-sen University Guangzhou 510275 China

Objective： To evaluate transduction efficiency with recombinant adenovirus-mediated p53 （rAd/p53） therapy in a human colon cancer mouse model by intra-tumoral injection and intra-arterial delivery. Methods： The tumor pieces of human colon cancer SW480 were implanted in the livers of 45 nude mice. These mice were administrated with rAd/p53 by intratumoral injection and intra-artedal delivery. After 24 h, 48 h and 72 h tAd/p53 administration, 5 mice each group were killed with over anesthesia and their livers were removed. P53 expression and apoptosis of tumor and liver were assessed. Results： P53 expression and apoptosis of intratumoral administration group was higher than tail vein group and control group. Apoptosis and p53 expression of livers in three groups had no significant difference. Conclusion： p53 gene transducUon efficiency and anticancer effect of rAd/p53 is much better by intra-tumoral injection than intra-arterial delivery,

关键词： nude mouse human colon cancer hepatic allograft model rAd/p53

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Ovostatin 2 knockdown significantly inhibits the growth,migration,and tumorigenicity of cutaneous malignant melanoma cells

Ovostatin 2 knockdown significantly inhibits the growth,migr...

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江苏省遗传学会2016年学术年会

作者： Ying-Xue Huang Hao Song Xue-Bao Shao Xue-Si Zeng Xiu-Lian Xu Jinliang Qi Jian-Fang Sun Key Laboratory of Molecular Biology for Skin Diseases Jiangsu ProvinceInstitute of DermatologyChinese Academy of Medical Sciences Department of Dermatology Xiangya HospitalCentral South University State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing University

We previously identified ovostatin 2 as a new candidate gene of cutaneous malignant melanoma in a Chinese *** the current study,we aim to investigate the exact role of ovostatin 2 in cell proliferation,invasion,and tumorigenesis of melanoma A375 ***-regulation of ovostatin 2 expression was performed using lentiviral vectors with specific *** effects of ovostatin 2 expression on cell proliferation,cell cycle,cell migration,cell invasion,as well as on the potentialof tumorigenesis,were further *** results showed that down-regulation of ovostatin 2 significantly suppressed the proliferation of A375 cells and led to a G2/M phase *** chamber assay showed that the reduced expression ofovostatin 2 also significantly inhibited the transmigration of A375 *** blot results showed down-regulated expression of p-FAK,p-AKT,and p-ERK,accompanied with up-regulated epithelial phenotypes E-cadherin and β-catenin,and down-regulated mesenchymal phenotype N-cadherin expression after ovostatin 2 was knocked *** mouse transplantation tumor experiment showed,that after an observation period of 32 days,the growth speed and weight of transplanted tumors were significantly suppressed in the group of 6 BALB/c mice subcutaneously injected with ovostatin 2 knocked down A375 *** of ovostatin 2 had significantly suppressive effects on the proliferation,motility,and migration capabilities of A375 cells,suggesting a crucial promotive role of ovostatin 2 in the pathogenesis and progression of cutaneous malignant *** involved mechanisms may at least be partly associated with the over-activation of FAK/MAPK/ERK and FAK/PI3 K/AKT signals and the enhancement of the epithelial–mesenchymal transition.

关键词： A375 alpha-Macroglobulin Melanoma nude mouse shRNA

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