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INVESTIGATION OF TUMORIGENIC EFFECT ON ULTRAWEAK LUMINESCENCE FROM nude mouse FOR ANIMAL MODEL

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Chinese Journal of Cancer Research 1992年第4期4卷 49-53页

作者：马玉琴宋学玲赵克俭张仲纶马斌郑雁珍刘成祥苏震 Institute of Biophysics Chinese Academy of Sciences Beijing

With nude mouse for animal model, this paper has investigated its whole body luminescence after or before it was inoculated with tumor, and the relationship between tumorigenesis and the luminescence, then, studied the luminescence difference from different mouse organs and find out sensitive luminescence organs. Furthermore, it has tested possibie luminescence mechanism with beta- carotene, NaN3 and D2O. This paper is supposed to provide a new feasible method for cancer diagnosis.

关键词： low level chemiluminescence nude mouse tumor.

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Orthotopic graft model of Transplantable Human Glioma tissue in nude mouse Brain

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Orthotopic graft model of Transplantable Human Glioma tissue...

裸体小鼠(nude mouse)的繁育和大量生产(二)

中国抗癌协会神经肿瘤专业委员会第八届学术会议

作者：李如军董军黄强苏州大学附属第二医院神经外科

BACKGROUND ＆ OBJECTIVE:In previous studies,most methods of orthotopic transplantation model of glioma were injecting cell suspension into mouse *** this operation is complex,time-consuming,and difficult to construct in a large scale within a short *** study was designed to improve human glioma xenografts implanted intracranial in nude mice by tumor tissue ***:Firstly,human glioma cells were injected subcutaneously into nude mice to form human glioma *** the transplantable human glioma xenografts（2 mm3） were put into trocar and directly injected into caudate nucleus of nude mice（fig.）.HE staining and MRI scanning were performed to investigate the histopathological and imaging features of this *** days later,the tumor were detected by 1.5T superconduct MR with micro-23 *** data of tumors were compared with H＆E sections data to evaluate the feasibility of MR imaging in tumor ***:It takes only less than 3minutes to implant human glioma xenografts in one nude *** 14 of 15 orthotopic grafted nude mice,xenograft tumors could be detected by MRI,which were also in line with the results observed under microscope;what’s more,the tumor shape,position and size in MR were accord with frozen section（p＞0.05）.The transplantation success rate was 93%（14/15）.CONCLUSION:Tissue quantitative implantation via trocar is simple,timesaving,high successful rate,and easy to construct model in large scale.

关键词： Brain neoplasm/glioma nude mouse Orthotopic graft model Orthotopic xenograft

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实验动物与比较医学 1981年第2期 105-108页

作者： Ediger,R. Giovanella,B.C. 漆畹生

供食和供水一、饲喂(接上期)裸体小鼠的营养需求与其他品系的小鼠相似。所以,任何市售饲块都可以使用。食物必须灭菌。可将食物铺成单层后放在小盘中或放在有孔的口袋中,进行高压蒸汽灭菌。灭菌前,不耐热的营养素要加大量(Williams 氏等... 详细信息

供食和供水一、饲喂(接上期)裸体小鼠的营养需求与其他品系的小鼠相似。所以,任何市售饲块都可以使用。食物必须灭菌。可将食物铺成单层后放在小盘中或放在有孔的口袋中,进行高压蒸汽灭菌。灭菌前,不耐热的营养素要加大量(Williams 氏等,1968)。灭菌后的食品应当在90天内用完。可补充全小麦面包和复制牛奶以改进生育性能,并防止同类噬食现象(Poiley 氏等,1974)。本文作者之一(Giovanella 氏)也认为补充上述食物可抑

关键词：小鼠小家鼠 nude mouse 雌鼠

裸小鼠(nude mouse)的繁育及其在肿瘤实验治疗中的应用

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国际药学研究杂志 1982年第5期 257-263页

作者：张素胤胥彬中国科学院上海药物研究所

过去在实验肿瘤研究中多用动物肿瘤模型作为实验材料,但它与人体肿瘤的生物学特性有很大差异,因此研究结果往往不能反映人体肿瘤本质。这种现象在抗癌药物研究中尤为突出,例如对动物肿瘤有效药物,经临床试用结果相关性较差,因此多年来... 详细信息

过去在实验肿瘤研究中多用动物肿瘤模型作为实验材料,但它与人体肿瘤的生物学特性有很大差异,因此研究结果往往不能反映人体肿瘤本质。这种现象在抗癌药物研究中尤为突出,例如对动物肿瘤有效药物,经临床试用结果相关性较差,因此多年来科研工作者一直响往用人体肿瘤模型进行实验肿瘤的研究,这一理想由于裸小鼠(nude mouse,我国又称无毛、无胸腺小鼠)的发现而成为现实。1966年Flanagan首次报道一种无毛突变型小鼠并定名为裸小鼠(缩写nu/nu)。Pantelouris

关键词：人体肿瘤细胞株裸小鼠实验动物实验治疗 nude mouse

一种对免疫和肿瘤研究有价值的动物——先天性无胸腺小鼠(nude mouse)

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遵义医科大学学报 1979年第1期 125-129页

作者：张颖悟遵义医学院微生物学教研组

胸腺是主要的免疫器官之一,它在免疫系统中的作用是:一方面向其他淋巴样组织输送T淋巴细胞,另一方面对来自其他免疫器官的前体T淋巴细胞进行诱导,使其分化成T淋巴细胞。关于胸腺以及与此有关的免疫机制和免疫反应等的研究,以往主要是用... 详细信息

胸腺是主要的免疫器官之一,它在免疫系统中的作用是:一方面向其他淋巴样组织输送T淋巴细胞,另一方面对来自其他免疫器官的前体T淋巴细胞进行诱导,使其分化成T淋巴细胞。关于胸腺以及与此有关的免疫机制和免疫反应等的研究,以往主要是用摘除胸腺或X线照射的办法在动物身上进行的。这些方法虽然在近代免疫学的研究上起了一定的作用,但却不可能排除在胎儿时期已体现作用的胸腺的影响。在肿瘤的实验研

关键词：小鼠无胸腺小家鼠胸腺发育 nude mouse 动物模型肿瘤研究

裸体小鼠(nude mouse)的繁育和大量生产(一)

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实验动物与比较医学 1981年第1期 52-54页

作者： Ediger,R. Giovanela,B.C. 漆畹生

引言要想生产高质量的裸体小鼠,要求实验设备的精心设计和一定条件,再加上合理措施。具有正常免疫力的小鼠能够抵抗某些细菌和病毒,裸体小鼠对它们却是敏感的,因

关键词：小鼠小家鼠 nude mouse 层流室

Development and characterization of multidrug resistant human hepatocarcinoma cell line in nude mice

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World Journal of Gastroenterology 2006年第41期12卷 6614-6619页

作者： Bao-Jin Zhai Ze-Yong Shao Chun-Liang Zhao Kai Hu Feng Wu Clinical Center for Tumor Therapy of 2nd Affiliated Hospital and Institute of Ultrasonic Engineering in Medicine Chongqing University of Medical Sciences Chongqing China

AIM： To establish a multidrug resistant （MDR） cell subline from the human hepatocardnoma cell line （HepG2） in nude mice. METHODS： HepG2 cell cultures were incubated with increasing concentrations of adriamycin （ADM） to develop an ADM-resistant cell subline （HepG2/ADM） with crossresistance to other chemotherapeutic agents. Twenty male athymic BALB/c-nu/nu mice were randomized into HepG2/nude and HepG2/ADM/nude groups （10 in each group）. A cell suspension （either HepG2 or HepG2/ADM） was injected subcutaneously into mice in each group. Tumor growth was recorded, and animals were sacrificed 4-5 wk after cell implantation. Tumors were prepared for histology, and viable tumor was dispersed into a single-cell suspension. The IC50 values for a number of chemotherapeutic agents were determined by 2, 3-bis （2-methoxy-4-nitro-5-sulfophenyl）-2H-tetrazolium-5-carboxanilide inner salt （MTT） assay. Rhodamine-123 retention/efflux and the level of resistance-associated proteins were determined by flow cytometry. The mRNA expression of mdr1, mrp and Irp genes was detected using reverse transcriptase polymerase chain reaction （RT-PCR） in HepG2/nude and HepG2/ADM/nude groups. RESULTS： The appearances of HepG2/nude cells were slightly different from those of HepG2/ADM/nude cells. Similar tumor growth curves were determined in both groups. A cross-resistance to ADM, vincristine, cisplatin and 5-fluorouracil was seen in HepG2/ADM/nude group. The levels of P-glycoprotein and multidrug resistanceassociated proteins were significantly increased. The mRNA expression levels of mdrl, mrp and/rp were higher in HepG2/ADM/nude cells. CONCLUSION： ADM-resistant HepG2 subline in nude mice has a cross resistance to chemotherapeutic drugs. It may be used as an in vivo model to investigate the mechanisms of MDR, and explore the targeted approaches to overcoming MDR.

关键词： Hepatocarcinoma Multidrug resistance Adriamycin Model nude mouse

Silencing of signal transducer and activator of transcription 3 expression by RNA interference suppresses growth of human hepatocellular carcinoma in tumor-bearing nude mice

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World Journal of Gastroenterology 2009年第21期15卷 2602-2608页

作者： Jing Li Yun-Feng Piao Zheng Jiang Li Chen Hai-Bo Sun Department of Gastroenterology the First Affiliated Hospital of Liaoning Medical University Jinzhou 121000 Liaoning Province China Department of Gastroenterology the First Hospital of Jilin University Changchun 130021 Jilin Province China Department of Orthopedics the Second Hospital of Jinzhou City Jinzhou 121003 Liaoning Province China

AIM： To explore the effect of silencing of signal transducer and activator of transcription 3 （STAT3） expression by RNA interference （RNAi） on growth of human hepatocellular carcinoma （HCC） in tumorbearing nude mice in vivo.METHODS： To construct the recombinant plasmid of pSilencer 3.0-H1-STAT3-siRNA-GFP （pSHI-siRNA- STAT3） and establish the tumor-bearing nude mouse model of the HCC cell line SMMC7721, we used intratumoral injection together with electroblotting to transfect the recombinant plasmid pSHI-siRNA- STAT3 into the transplanted tumor. The weight of the nude mice and tumor volumes were recorded. STAT3 gene transcription was detected by semi-quantitative reverse transcription polymerase chain reaction （RT- PCR）. Level of protein expression and location of STAT3 were determined by Western blotting and immunohistochemical staining. STAT3-related genes such as survivin, c-myc, VEGF, p53 and caspase3 mRNA and protein expression were detected in tumor tissues at the same time. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to detect apoptosis of tumor cells.RESULTS： The weight of the treated nude mice increased, and the tumor volume decreased markedly compared with those of the mock-treated and negative control groups （P 〈 0.01）. The results of RT-PCR and Western blotting showed that mRNA and protein levels of STAT3 declined markedly in the treated group. The change in STAT3-related gene expression in tumor tissues at the mRNA and protein level also varied, the expression of survivin, VEGF and c-myc were obviously reduced, and expression of p53 and caspase3 increased （P 〈 0.01）. Most of the tumor tissue ceils in the treated group developed apoptosis that was detected by TUNEL assay.CONCLUSION： Silencing of STAT3 expression by RNAi significantly inhibits expression of STAT3 mRNA and protein, and suppresses growth of human HCC in tumor-bearing nude mice. The mechanism may be related to down-regulation of survi

关键词： RNA interference Signal transducerand activator of transcription 3 transcription factor Hepatocellular carcinoma Xenograft model antitumorassays nude mouse

Intravesical Instillation in Pure Line LEW Rats and nude Mice

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第3期27卷 288-290页

作者：周洁谢蜀生郭晓云莫曾南 Department of Urology the First Affiliated Hospital of Guangxi Medical University The Laboratory of Immunopharmacology Health Science Center Peking University Department of Gastroenterology the First Affiliated Hospital of Guangxi Medical University Department of Urology 3Department of Gastroenterology the First Affiliated Hospital of Guangxi Medical University

In order to study bladder intravesical instillation methods in pure line LEW rats and nude mice, female LEW rats and nude mice aged 2 to 4 weeks were sacrificed. Their urethra and bladder were observed under anatomical microscopy. A trochar was prepared according to the outline and an- gle of the urethra. Ink was poured into female rats and nude mice bladder though urethra. Filling and staining of bladder were observed and evaluated under anatomical microscopy. Status and urethral injury of rats and mice were observed. The results showed that urethra anatomic structure of rats and nude mice was different from that of human urethra. When bladder was filled with ink and became blue, liquid was not seen to leak out. The success rate of intubation was high (100%). Living activi- ties of animals weren’t influenced by intravesical instillation. It was concluded that bladder irrigation might be a kind of valid and utilizable method in pure line rat and nude mouse empirical study. The model may be a more effective tool for study of bladder tumor.

关键词： intravesical instillation bladder neoplasms animal model nude mouse

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