检索结果-南通市图书馆

Circuit-Specific Control of Blood Pressure by PNMT‑Expressing Nucleus tractus solitarii Neurons

神经科学通报:英文版 2023年第8期39卷 1193-1209页

作者： Shirui Jun Xianhong Ou Luo Shi Hongxiao Yu Tianjiao Deng Jinting Chen Xiaojun Nie Yinchao Hao Yishuo Shi Wei Liu Yanming Tian Sheng Wang Fang Yuan Department of Neurobiology Hebei Medical UniversityShijiazhuang 050017China Institute of Cardiovascular Research Southwest Medical UniversityLuzhou 646000China Core Facilities and Centers Institute of Medicine and HealthHebei Medical UniversityShijiazhuang 050017China Hebei Key Laboratory of Neurophysiology Shijiazhuang 050017China

The Nucleus tractus solitarii(NTS)is one of the morphologically and functionally defined centers that engage in the autonomic regulation of cardiovascular activity.Phenotypically-characterized NTS neurons have been implicated in the differential regulation of blood pressure(BP).Here,we investigated whether phenylethanolamine N-methyltransferase(PNMT)-expressing NTS(NTS^(PNMT))neurons contribute to the control of BP.We demonstrate that photostimulation of NTS^(PNMT)neurons has variable effects on BP.A depressor response was produced during optogenetic stimulation of NTS^(PNMT)neurons projecting to the paraventricular nucleus of the hypothalamus,lateral parabrachial nucleus,and caudal ventrolateral medulla.Conversely,photostimulation of NTS^(PNMT)neurons projecting to the rostral ventrolateral medulla produced a robust pressor response and bradycardia.In addition,genetic ablation of both NTS^(PNMT)neurons and those projecting to the rostral ventrolateral medulla impaired the arterial baroreflex.Overall,we revealed the neuronal phenotype-and circuit-specific mechanisms underlying the contribution of NTS^(PNMT)neurons to the regulation of BP.

关键词： Nucleus tractus solitarii Blood pressure Rostral ventrolateral medulla Optogenetics Neural circuit

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Neural Circuit Mechanism Controlling Breathing by Leptin in the Nucleus tractus solitarii

引用

Neuroscience Bulletin 2022年第2期38卷 149-165页

作者： Hongxiao Yu Luo Shi Jinting Chen Shirui Jun Yinchao Hao Shuang Wang Congrui Fu Xiang Zhang Haiyan Lu Sheng Wang Fang Yuan Department of Physiology Hebei Medical UniversityShijiazhuang 050017HebeiChina Core Facilities and Centers Institute of Medicine and HealthHebei Medical UniversityShijiazhuang 050017HebeiChina School of Nursing Hebei Medical UniversityShijiazhuang 050000HebeiChina Department of Orthodontics College of StomatologyHebei Medical UniversityShijiazhuang 050017HebeiChina Hebei Key Laboratory of Neurophysiology Shiji azhuang 050017HebeiChina

Leptin,an adipocyte-derived peptide hormone,has been shown to facilitate breathing.However,the central sites and circuit mechanisms underlying the respiratory effects of leptin remain incompletely understood.The present study aimed to address whether neurons expressing leptin receptor b(LepRb)in the Nucleus tractus solitarii(NTS)contribute to respiratory control.Both chemogenetic and optogenetic stimulation of LepRb-ex-pressing NTS(NTS^(LepRb))neurons notably activated breathing.Moreover,stimulation of NTS^(LepRb) neurons projecting to the lateral parabrachial nucleus(LPBN)not only remarkably increased basal ventilation to a level similar to that of the stimulation of all NTS^(LepRb) neurons,but also activated LPBN neurons projecting to the preBotzinger complex(preBotC).By contrast,ablation of NTS^pRb neurons projecting to the LPBN notably eliminated the enhanced respiratory effect induced by NTSLepRb neuron stimulation.In brainstem slices,bath application of leptin rapidly depolarized the membrane potential,increased the spontaneous firing rate,and accelerated the Ca2+transients in most NTSLepRb neurons.Therefore,leptin potentiates breathing in the NTS most likely via an NTS-LPBN-preBdtC circuit.

关键词： Leptin Ventilation Nucleus tractus solitarii Neural circuit Chemogenetics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Role of the Nucleus tractus solitarii in the protection of pre-moxibustion on gastric mucosal lesions

引用

Neural Regeneration Research 2014年第2期9卷 198-204页

作者： Liang Peng Mi Liu Xiaorong Chang Zhou Yang Shouxiang Yi Jie Yan Yan Peng College of Acupuncture and Tuina Hunan University of Chinese Medicine

Previous studies have shown that somatic sensation by acupuncture and visceral nociceptive stimulation can converge in the Nucleus tractus solitarii where neurons integrate signals impact- ing on the function of organs. To explore the role of the Nucleus tractus solitarii in the protective mechanism of pre-moxibustion on gastric mucosa, Nucleus tractus solitarii were damaged in rats and pre-moxibustion treatment at the Zusanli （ST36） point followed. The gastric mucosa was then damaged by the anhydrous ethanol lavage method. Morphological observations, enzyme linked immunosorbent assays, and western immunoblot analyses showed that gastric mucosa surface lesion and the infiltration of inflammatory cells were significantly ameliorated after pre-moxibustion treatment. Furthermore, the gastric mucosal damage index and somatostatin level were reduced, and epidermal growth factor content in the gastric mucosa and heat-shock protein-70 expression were increased. These results were reversed by damage to the Nucleus tractus solitarii. These findings suggest that moxibustion pretreatment at the Zusanli point is protective against acute gastric mucosa injury, and Nucleus tractus solitarii damage inhibits these responses. Therefore, the Nucleus tractus solitarii may be an important area for regulating the signal transduction of the protective effect of pre-moxibustion on gastric mucosa.

关键词： nerve regeneration traditional Chinese medicine moxibustion Nucleus tractus solitarii gastric mucosal lesion heat shock protein-70 epidermal growth factor somatostatin NSFC grant neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Catecholamine inputs to expiratory laryngeal motoneurons in rat

Catecholamine inputs to expiratory laryngeal motoneurons in ...

引用

中国药理学会第十一次全国学术会议

作者： Robert G.BERKOWITZ Paul M.PILOWSKY Australian School of Advanced Medicine Macquarie UniversityNSW 2109Australia Departments of Otolaryngology and Paediatrics Royal Children's HospitalParkvilleVIC 3161Australia

OBJECTIVE Many motoneurons and respiratory neurons are demonstrated to receive catecholamine inputs,but the extent and distribution of these inputs to expiratory laryngeal motoneurons(ELM) in the caudal nucleus ambiguus(NA) is unknown. In the present study,we aim to:1) examine whether or not close appositions exist between tyrosine hydroxylase immunoreactive(TH-ir, ie,catecholaminergic) boutons and ELM by using intracellular labelling combined with immunohistochemistry,and,if so,identify the origins of the inputs,and,if yes,2) identify the potential origins of the catecholamine inputs by using tract-tracing study.METHODS We examined catecholamine inputs to ELM using a combination of intracellular labelling and immunohistochemistry in adult Sprague-Dawley rat.ELM were identified in vivo by their post-inspiratory firing pattern and antidromic responses to electrical stimulation of the recurrent laryngeal nerve.Further tract-tracing study was carried by injecting cholera toxinβ(CTB) into the caudal NA. The retrogradely labelled neurons and the TH-ir structures were revealed by dual fluorescence immunohistochemistry.RESULTS TH-ir close appositions were found on all ELMs,with an average number of 18±5 per neuron(n=7,mean±SD).Close appositions were commonly observed on the distal dendrites rather than on proximal ones,but none on somata or axons.Further tract-tracing study revealed that a large number of CTB labelled neurons in the ipsilateral Nucleus tractus solitarii(NTS) and area postrema(AP) were cat-echolaminergic, and the highest density appeared at 0.2 -0.4 mm caudal to the obex.CONCLUSION We demonstrated for the first time that the ELM receive TH-ir close appositions,suggesting that the catecholamine inputs may play a role in the activity of ELM.The afferents of the superior laryngeal nerve(SLN) project to the NTS neurons at the same longitudinal level,our further tract-tracing study suggests that catecholaminergic neurons located in the NTS at the obex level may play an important role in control of the SLN-med

关键词： laryngeal motoneurons tyrosine hydroxylase nucleus ambiguus Nucleus tractus solitarii intracellular recording

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：