检索结果-南通市图书馆

Development of nucleic acid sequence-based amplification Assay for Detection of Macrobrachium rosenbergii Nodavirus

Agricultural Biotechnology 2014年第3期3卷 42-45页

作者： Feng LIN Li LIU Dong QIAN Guijie HAO Pengcheng SHENG Zheng CAO Xuemei YUAN Jinyu SHEN Key Laboratory of Healthy Freshwater Aquaculture Ministry of AgricultureZhejiang Institute of Freshwater Fisheries School of Marine Sciences Ningbo University

A nucleic acid sequence-based amplification（NASBA）assay was established for the detection of Macrobrachium rosenbergii Nodavirus（MrNV）.The specific primers were designed according to the high conserved region of RNA2 sequence of *** 224 bp specific amplification product was obtained in positive sample determined with 3%agarose gel electrophoresis,while no product was generated from shrimp infected with other viruses including DNA viruses（IHHNV,WSSV）and RNA viruses（TSV,IMNV,YHV）.The detecting limit of the assay was 8pg nucleic acid,which is more sensitive than that of PCR method.

关键词： Macrobrachium rosenbergii Nodavirus nucleic acid sequence-based amplification Detection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research progress and prospects of nucleic acid isothermal amplification technology

引用

BIOCELL 2023年第11期47卷 2385-2395页

作者： SHUHUI WU PING XU XIANGBIN XU SONG-BAI LIU Suzhou Key Laboratory of Medical Biotechnology Suzhou Vocational Health CollegeSuzhou215009China School of Food Science and Engineering Hainan UniversityHaikou570228China The Affiliated Infectious Diseases Hospital Suzhou Medical College of Soochow UniversitySuzhou215100China

nucleic acid(DNA and RNA)detection and quantification methods play vital roles in molecular *** the development of molecular biology,isothermal amplification of DNA/RNA,as a new molecular biology technology,can be amplified under isothermal condition,it has the advantages of high sensitivity,high specificity,and high efficiency,and has been applied in various fields of biotechnology,including disease diagnosis,pathogen detection,food hygiene and safety detection and so *** paper introduces the progress of isothermal amplification technology,including rolling circle amplification(RCA),nucleic acid sequence-dependent amplification(NASBA),strand displacement amplification(SDA),loop-mediated isothermal amplification(LAMP),helicase-dependent amplification(HDA),recombinase polymerase amplification(RPA),cross-priming amplification(CPA),and its principle,advantages and disadvantages,and application development are briefly summarized.

关键词： Isothermal amplification Rolling circle amplification nucleic acid sequence-based amplification Strand displacement amplification Loop-mediated isothermal amplification Helicase-dependent amplification Recombinase polymerase amplification Cross-primer amplification

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：