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Plantlets Regeneration via Somatic Embryogenesis from the nucellus tissues of Kinnow Mandarin (Citrus reticulata L.)

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American Journal of Plant Sciences 2016年第6期7卷 798-805页

作者： Mubashir Hussain Naveed Iqbal Raja Muhammad Iqbal Anam Iftikhar Huma Mehreen Sadaf Sidra Sabir Muhammad Asim Sultan Muhammad Nasim Ashraf Faz Department of Botany PMAS Arid Agriculture University Rawalpindi Pakistan Department of Botany University of Sargodha Sargodha Pakistan Department of Botany Government College University Faisalabad Pakistan

Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. nucellus tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins, cytokinins and malt extract for primary callus induction. The best response for primary callus induction (90%) was obtained when MS medium was supplemented with 5 mg/l 2,4-D and 500 mg/l malt extract. Best results for embryogenic callus induction (80%) were obtained in C8 medium. The induction of somatic embryos was highest when MS medium was supplemented with 1 mg/l BAP and maturation of somatic embryos occurred when MS medium was supplemented with 5 mg/l 2,4-D and 1 mg/l BAP. Maximum plantlets were regenerated (92%) from the somatic embryos on half strength MS medium with no hormones. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (100%) was achieved in potting mixture containing sand and peat moss (2:1).

关键词： tissue Culture MS Media Citrus nucellus tissue Callogenesis Somatic Embryos

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