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NADPH oxidase 2 does not contribute to early reperfusion-associated reactive oxygen species generation following transient focal cerebral ischemia

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Neural Regeneration Research 2016年第11期11卷 1773-1778页

作者： Yuan Zhang Ting Wang Ke Yang Ji Xu Jian-ming Wu Wen-Ian Liu Central Laboratory Shenzhen Second People's HospitalFirst Affiliated Hospital of Shenzhen University Shenzhen Key Laboratory of Neurosurgery Shenzhen Second Peoples HospitalFirst Affiliated Hospital of Shenzhen University Department of Pathophysiology Baotou Medical College Graduate School of Guangzhou Medical University Department of Neurosurgery Shenzhen Second People's HospitalFirst Affiliated Hospital of Shenzhen University

Excess production of reactive oxygen species （ROS） critically contributes to occurrence of reperfusion injury, the paradoxical response of ischemic brain tissue to restoration of cerebral blood flow. However, the enzymatic sources of ROS generation remain to be unclear. This study examined nox2-ontaining NADPH oxidase （nox2） expression and its activity in ischemic brain tissue following post-ischemic reperfusion to clarify the mechanism of enzymatic reaction of ROS. Male Sprague-Dawley rats were subjected to 90-minute middle cerebral artery occlusion, followed by 3 or 22.5 hours of reperfusion. Quantitative reverse transcriptase PCR and western blot assay were performed to measure mRNA and protein expression of nox2. Lucigenin fluorescence assays were performed to assess nox activity. Our data showed that nox2 mRNA and protein expression levels were significantly increased （3.7-fold for mRNA and 3.6-fold for protein） in ischemic brain tissue at 22.5 hours but not at 3 hours following post-ischemic reperfusion. Similar results were obtained for the changes of NADPH oxidase activity in ischemic cerebral tissue at the two reperfusion time points. Our results suggest that nox2 may not contribute to the early burst of reperfusion-related ROS generation, but is rather an important source of ROS generation during prolonged reperfusion.

关键词： nerve regeneration NADPH oxidase cerebral ischemia nox family reactive oxygen species reperfusion central nervous system stroke blood flow neural regeneration

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