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Relationship between notch receptors and Hyperoxia-induced Lung Injury in Newborn Rats

Journal of Huazhong University of Science and Technology(Medical Sciences) 2005年第2期25卷 155-158页

作者：张谦慎常立文刘汉楚容志惠陈红兵 Department of Pediatrics. Tongji Hospital. Tongji Medical College. Huazhong Universityof Science and Technology. Wuhan 430030. China

Summary: To investigate role of notch1-3 in hyperoxia-induced lung injury in newborn rat exposed to 85% O 2, SD rat litters born on the 22th day were randomly divided into two groups: room air group and hyperoxia group. The animals were sacrificed 1, 4, 7, 10, 14 and 21 days after continued exposure to oxygen (n=40, oxygen>0.85) or room air (n=40). 6 rats each group were used to assess lung histological changes by HE staining and expression of notch in lungs by immunohistochemistry. Total RNA was extracted by Trizol reagent from frozen lung tissues. notch mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR). Our results showed that 7, 14 and 21 days after O 2 exposure, hyperoxia group showed lung injury characterized by pulmonary edema, hemorrhage and lung development arrest. Positive staining for notch1, notch 2 in hyperoxia group was much lower than those in room air group at all time points (Pnotch3 (P>0.05). Immunostained cells were typically airways epithelia, alveolar epithelial and inflammatory cells, and fibroblasts in hyperoxia group (Pnotch mRNA levels showed similar change as protein level (Pnotch receptors, which might contribute to the pathogenesis of hyperoxia-induced lung injury in newborn rats. The decreased inhibition of notch1 might be one of the protective reaction and major mechanisms for proliferation/differentiation of type Ⅱ alveolar epithelial cells. The up-regulation of notch3 activity might result in the lung development arrest of the newborn rats.

关键词： notch receptor hyperoxia lung injury newborn

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Late SV40 factor:A key mediator of notch signaling in human hepatocarcinogenesis

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World Journal of Gastroenterology 2011年第29期17卷 3420-3430页

作者： Ren-Hua Fan Jing Li Nan Wu Ping-Sheng Chen Department of Pathology School of MedicineSoutheast UniversityNanjing 210009Jiangsu ProvinceChina

AIM:To investigate the relationship between late SV40 factor(LSF)and notch signaling in the development and progress of hepatocellular carcinoma(HCC).METHODS:Liver cancer tissue specimens from 25 patients were analyzed for notch-1 and LSF expression by *** correlation between expression and the biological effects of notch-1 and LSF were analyzed using genetic and pharmacological strategies in HCC cell lines and human normal cell lines,including hepatic stellate cells(HSC)and human embryonic kidney epithelial cells(HEK).RESULTS:Immunohistochemistry showed that both notch-1 and LSF were significantly upregulated in HCC samples(76%,19/25,Pnotch-1 by exogenous transfection of notch1 intracellular domain increased LSF expression in HSC and HEK cells to levels similar to those seen in HepG2 ***,blocking notch-1 activation with aγ-secretase inhibitor,DAPT,downregulated LSF expression in HepG2 ***,a biological behavior assay showed that forced overexpression of LSF promoted HepG2 cell proliferation and ***:LSF is a key mediator of the notch signaling pathway,suggesting that it might be a novel therapeutic target for the treatment of HCC.

关键词： notch receptor Late SV40 factor Signal transduction Hepatocellular carcinoma

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The expression of NICD in cervical cancers and the influence of DAPT on cervical cancer cell lines

The expression of NICD in cervical cancers and the influence...

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2008中国国际妇科肿瘤会议

作者：孙晓梅 Department of Obstetrics ＆ Gynecology Peking University First Hospital

＜正＞OBJECTIVE To study the NICD expression in cervical cancer and its clinical significance,and the effects of DAPT,aγ-secretase inhibitor on the proliferation and apoptosis of cervical cancer cell *** Western

关键词： cervical cancer γ-secretase inhibitor DAPT NICD notch receptor

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