检索结果-南通市图书馆

Journal of Integrative Plant Biology 2005年第6期47卷 703-708页

作者： HuiHUANG Ke-ZhiBAI Ze-PuZHONG Liang-BiLI Ting-YunKUANG KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiology InstituteofBotanytheChineseAcademyofSciencesBeijing100093China

: During the non-frost season, the condensation of dew makes nostoc flagelliforme Born. et Flah., a highly drought-tolerant terrestrial cyanobacterium, frequently undergo rehydration-dehydration. Rehydration begins in the dark at night. After rewetting in the dark, photochemical activity and the structure of photosystem (PS) II were not recovered at all; the structure of PSI, energy transfer in phycobilisomes, and energy transfer from phycobilisomes to PSI were recovered within 5 min, as in the light. The recovery of energy transfer from phycobilisomes to PSII was light dependent and energy transfer from phycobilisomes to PSII was only partially recovered in the dark. These results suggest that the two-trigger control (water and light) of photo synthetic recovery may make N. flagelliforme avoid unnecessary energy consumption and, at the same time, the partial recovery of energy transfer from phycobilisomes to PSII in the dark could help N. flagelliforme accumulate more photosynthetic products during the transient period of rehydration-dehydration.

关键词： dark energy transfer nostoc flagelliforme photosystem II phycobilisome rewetting

ULTRASTRUCTURE OF THE VEGETATIVE CELLS OF nostoc flagelliforme PREPARED WITH HIGH PRESSURE FREEZING AND FREEZE SUBSTITUTION TECHNIQUE 

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Botanica Sinica 1998年第10期40卷 901-905页

作者：祝建王俊华振基马丁.米勒上海铁道大学基础医学院宁夏农学院生物系瑞士联邦理工大学电子显微镜技术实验一室

The ultrastructure of the vegetative cells of nostoc flagelliforme Born. et Flah. was investigated with high pressure freezing and freeze substitution technique and compared with the results obtained by using conventional preparation methods. During the processes of chemical fixation, dehydration and embedding, the cell structures might be more artificially modified than that obtained from high pressure freezing and freeze substitution. With the present method, the sheath of N. flagelliforme could be well penetrated and no extra big space could exist between the cell and the sheath. The cell protoplasm rarely shrinked. Some fine structures of cell inclusions and unit membranes became visualized. Many bacteria were harbored in the sheath. In addition, the presence of big vacuoles in the cell of N. flagelliforme as well as the presence of bacteria in the sheath shown in the present preparation for cyanobacteria has not been described so far in the literature.

关键词： Ultrastructure nostoc flagelliforme Vegetative cell High pressure freezing Freeze substitution

Membrane Lipids and Their Fatty Acid Composition in nostoc flagelliforme Cells

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Acta Botanica Sinica 2000年第12期42卷 1263-1266页

作者：王梅许亦农姜桂珍李良璧匡廷云中国科学院植物研究所光合作用基础研究开放实验室北京100093

nostoc flagelliforme Born. et Flah is highly adapted to drought stress, cold and light stresses, and suitable for growing in the unfavorable areas. This paper presents the results of the analysis of the membrane (mainly thylakoid membrane) lipids from N. flagelliforme in order to investigate the relationship between membrane lipid composition and stress resistance to this cyanobacteria. The membrane lipids are composed of monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), sulfoquinovosyl diacylglycerol (SQDG) and phosphatidylglycerol (PG). The major fatty acids in these lipids are palmitic (16∶0), palmitoleic (16∶1), stearic (18∶0), oleic (18∶1), linoleic (18∶2) and linolenic (18∶3) acids. In N. flagelliforme , polyunsaturated fatty acids account for 73% of the total fatty acids, much higher than that of the other cyanobacteria reported so far. Among which 16∶1 and 18∶3 are as high as 28.9% and 34.3% respectively. The high resistance of N. flagelliforme to abnormal conditions may be associated with the extent of unsaturation of fatty acids. In addition, the wild N. flagelliforme treated with water for 30 min and cultured for 24 h and the lipid and fatty acid composition were found to be not affected by water_absorption.

关键词： nostoc flagelliforme membrane lipids fatty acids cold resistance

Molecular Cloning and Characterization of Maltooligosyltrehalose Synthase gene from nostoc flagelliforme

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Cloning and Characterization of Maltooligosyltreha...

庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： Shuangxiu Wu,Rongrong Shen,Xiu Zhang,Quanxi Wang~* (Department of Biology,College of Life and Environmental Science,Shanghai Normal University,Guilin Road 100,Shanghai,200234,People’s Republic of China)

A genomic DNA fragment encoding a putative maltooligosyltrehalose synthase (NfMTS) for trehalose biosynthesis was cloned by the degenerate primer-PCR from cyanobacterium nostoc *** ORF of NfMTS is 2,799 bp in length and encodes 933 amino acid residues constituting a 106.6 kDa *** deduced amino acid sequence of NfMTS contains 4 regions highly conserved for *** expression of NfMTS in ***,the function of this protein was demonstrated that the recombinant protein catalyzed the conversion of maltohexaose to maltooligosyl *** Km of the recombinant enzyme for maltohexaose was 1.87 mM and the optimal temperature and pH of the recombinant enzyme was at 50℃and 7.0 *** expression of MTS of *** was upregulated and both trehalose and sucrose contents increased significantly in *** during drought ***,trehalose accumulated in small quantities,about 0.36 mg·g DW whereas sucrose accumulated in high quantities,about 0.90 mg·g DW, indicating both trehalose and sucrose involved in dehydration stress response in N. flagelliforme and sucrose might act as a chemical chaperone rather than trehalose did during dehydration stress.

关键词： nostoc flagelliforme maltooligosyltrehalose synthase degenerate primer desiccation stress cyanobacterium

Protein acetylation as a mechanism for nostoc flagelliforme adaptation to drought stress

在线全文

cnki会议

学校读者我要写书评

暂无评论

Protein acetylation as a mechanism for Nostoc flagelliforme ...

中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Lingxia Wang Yang Liu Fengkun Yan Xiaoxu Li Miaomiao Ding Men Wang Jing Shi Tingting Xu Wenyu Liang School of Life Sciences Ningxia University Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China School of Agriculture Ningxia University

nostoc flagelliforme,a terrestrial cyanobacterium,is well adapted to *** explore the adaptation mechanisms of *** in response to drought condition,a large-scale quantitative lysine acetylome was carried out using immunoaffinity enrichment of Lys-acetylated peptides and liquid chromatography linked to tandem mass spectrometry.A total of 1340 lysine acetylation（LysAc） sites,1340 Lysacetylated peptides and 653 Lys-acetylated proteins were *** conserved sequence motifs of the LysAc sites,including a new motif KxxK,were *** analyses revealed that the differentially acetylated proteins involved in response to drought condition were preferentially predicted to participate in glutathione metabolism and carbon metabolism and photosynthesis pathways based on KEGG pathway category enrichment *** found that GSH accumulation played important roles as stress protectant and reactive oxygen species scavenger,and soluble sugar accumulation improved the osmotic regulation ability in response to drought condition in *** energy metabolism-related proteins of *** were regulated by the LysAc modification under the environment of extreme drought to maintain lower *** with a large number of accumulation the energy metabolism of raw materials（glucose,pyruvic acid） are advantageous to quickly restore physiological activity under the condition of *** of physiological parameters and mRNA expression levels related to glutathione metabolism and carbon metabolism and photosynthesis were found to be significantly affected by drought *** together,reversible protein LysAc is likely to be a post-translational mechanism contributing to the ***’s adaptation to drought condition.

关键词： nostoc flagelliforme drought condition protein acetylation

Analysis of differentially expressed antioxidant proteins in the drought-tolerant cyanobacterium,nostoc flagelliforme subjected to desiccation

在线全文

cnki会议

学校读者我要写书评

暂无评论

Analysis of differentially expressed antioxidant proteins in...

生态文明建设中的植物学：现在与未来——中国植物学会第十五届会员代表大会暨八十周年学术年会

作者： Wenyu Liang Lingxia Wang Jing Shi Guilian Mao Xiaoting Lei Jia Yang School of Life Sciences Ningxia University School of Life Sciences Fujian Agriculture and Forestry University

nostoc flagelliforme is a terrestrial nitrogen-fixing cyanobacterium which is distributed in arid or semiarid steppes in the western and north-western *** shows a strong ecological adaptability to xeric drought conditions.2-DE and MALDI-TOF/TOF-MS were conducted to separate and identify differentially expressed levels of antioxidant proteins in *** subjected to desiccation.A total of 5 kinds of antioxidant proteins(8 protein spots) that exhibited regulated expression were successfully identified,including peroxiredoxin,Mn-CAT,Fe-SOD,SOD and ferritin,which were all down-regulated except for peroxiredoxin when colonies were subjected to desiccation,these proteins may serve as molecular markers for *** when colonies were subjected to desiccation due to their obvious changes characteristics in expression ***,superoxide anion and H2O2 level,SOD,CAT and POD activities were increased firstly,and then markedly decreased when colonies were subjected to *** characteristics may contribute to our understanding of its survival strategy in dry habitats.

关键词： nostoc flagelliforme 2-DE Differentially expressed antioxidant proteins Identification.

Investigation of mechanism in Response to Rehydration for nostoc flagelliforme based on quantitative proteomics

在线全文

cnki会议

学校读者我要写书评

暂无评论

Investigation of mechanism in Response to Rehydration for No...

2018年中国质谱学术大会（CMSC2018）

作者： Bing Wang Jingjing Yang Cuihong Wan Hubei Key Lab of Genetic Regulation & Integrative Biology School of Life SciencesCentral ChinaNormal University

To adapt to xeric environments, microorganisms have evolved with the capability of the superior desiccation tolerance and rapid resuscitation after rehydration. N. flagelliforme, a representative terrestrial cyanobacterium that is distributed in west and west-northern parts of China, serves as an ideal model for gaining insight in the physiological recovery mechanism. In this study, LC-MS/MS combined with isobaric chemical labeling technique(i TRAQ) was used to quantify dynamic changes of proteins in N. flagelliforme during the rehydration processes. Approximately 113 proteins were identified to be di□erentially expressed, with function mainly related to photosynthesis, defense response, biosynthesis, antioxidant system, and energy and carbohydrate metabolism. Among them, protective proteins including high light inducible proteins and antioxidants showed a down regulation trend during the rehydration process, while proteins involved in photosynthesis, biosynthesis and signaling pathways and regulation of gene expression tend to be up-regulated. Signaling through Ser/Thr/Tyr phosphorylation has emerged as an important regulatory mechanism in cyanobacteria to sense environmental signals and implement adaptive changes. Furthermore, dynamic changes in transcription and protein levels have been studied, but what takes place in the extracellular environment to cope with changing environmental conditions and survive in various harsh environments remains largely unknown. Therefore, quantitative and qualitative analysis of the phosphorylated proteome and extracellular proteome were also performed. These results might shed light on molecular mechanism for the *** response to hydration.

关键词： nostoc flagelliforme rehydration iTRAQ phosphorylation extracelluar protein desiccation tolerance

Diverse functions of phosphorylation contributes to drought tolerance in nostoc flagelliforme facilitated by comparative phosphoproteomic

在线全文

cnki会议

学校读者我要写书评

暂无评论

Diverse functions of phosphorylation contributes to drought ...

中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Fengkun Yan Lingxia Wang Xiaoxu Li Miaomiao Ding Meng Wang Jun Wang Wenyu Liang School of Agriculture Ningxia University School of Life Sciences Ningxia University College education for Nationalities Ningxia University

Terrestrial cyanobacteria originate from aquatic *** must form a unique mechanism of drought adaptation during the long term ***,the mechanism remains *** this study,we carried out a global and sitespecific phosphoproteomic analysis on nostoc flagelliforme,a kind of terrestrial cyanobacteria being strong drought ecological *** total,329 phosphopeptides and 1168 phosphorylation sites from 224 proteins were identified through the combined use of protein/peptide prefractionation,TiO2 enrichment,and LC-MS/MS *** identified phosphoproteins were functionally categorized and found to be involved in various biological processes such as photosynthesis,ROS scavenging,cell wall synthesis and two-component signaling *** results revealed that the content of photosynthesis products,sucrose,trehalose and soluble sugar were significantly *** saccharides play a key role in osmotic *** also found that the content of glycogen was significantly *** result suggests that glycogen could serve as a supportive rather than a competitive carbon pool for the synthesis of *** treatment caused an increase in activity of antioxidant enzymes like Trx,CAT,APX and MDHAR with water *** data provide the first global survey of phosphorylation in terrestrial cyanobacteria by using phosphoproteomic *** provided data may help to reveal the physiological functions underlying phosphorylation and entire signaling networks in terrestrial cyanobacteria.

关键词： nostoc flagelliforme phosphoproteomic photosynthesis drought tolerance

陆生蓝藻发菜(nostoc flagelliforme)质膜和类囊体膜的分离和特性

在线全文

cnki会议

学校读者我要写书评

暂无评论

陆生蓝藻发菜(Nostoc flagelliforme)质膜和类囊体膜的分离和特性

全国植物光合作用、光生物学及其相关的分子生物学学术研讨会

作者：王可玢钟泽璞娄世庆赵福洪黄辉李良璧匡廷云中国科学院植物研究所光合作用研究中心中国科学院植物研究所光合作用研究中心中国科学院植物研究所光合作用研究中心中国科学院植物研究所光合作用研究中心中国科学院植物研究所光合作用研究中心中国科学院植物研究所光合作用研究中心中国科学院植物研究所光合作用研究中心

发菜即发状念珠藻(nostoc flagelliforme),分布于我国西北干旱和半干旱荒漠地区。它是一种陆地生蓝藻;它的藻丝体都包埋在胶质壳中。由于胶质壳的存在,其细胞难以破碎,因而也就难以获得发菜的质膜和类囊体膜。目前多数研究都以整体发菜... 详细信息

发菜即发状念珠藻(nostoc flagelliforme),分布于我国西北干旱和半干旱荒漠地区。它是一种陆地生蓝藻;它的藻丝体都包埋在胶质壳中。由于胶质壳的存在,其细胞难以破碎,因而也就难以获得发菜的质膜和类囊体膜。目前多数研究都以整体发菜为材料进行光合作用的研究。我们采用French Press机械压碎和蔗糖密度梯度离心等方法成功的破碎了胶质壳,并自发菜分离到两种类型的膜。一条是桔红色带,它的吸收光谱主要来自类胡萝卜素,其主要峰位和肩在439nm,489nm和521nm,并带有一小峰,位置在674nm,是少量叶绿素a的吸收峰。由于它的光谱特性和集胞藻6714的细胞质膜十分相似,我们认定这个桔红色部分为发菜的细胞质膜。另一条是绿色带,其吸收光谱主要来自叶绿素,其峰位和肩在416nm,441nm和683nm;另有类胡萝卜素,其肩位在490nm,这一部分可被认定为发菜的类囊体膜。

关键词： nostoc flagelliforme 类囊体膜

Aβ-Carotene Ketolase Gene NfcrtO from Subaerial Cyanobacteria Confers Drought Tolerance in Rice

在线全文

cnki会议

学校读者我要写书评

暂无评论

Rice science 2024年第1期31卷 62-76页

作者： GAO Ningning YE Shuifeng ZHANG Yu ZHOU Liguo MA Xiaosong YU Hanxi LI Tianfei HAN Jing LIU Zaochang LUO Lijun College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Shanghai Agrobiological Gene Center Shanghai 201106China College of Life Sciences Shangrao Normal UniversityShangrao 334001China Institute for Agri-food Standards and Testing Technology Shanghai Academy of Agricultural SciencesShanghai 201403China

nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme *** this study,we identified the drought tolerance gene NfcrtO,which encodes aβ-carotene ketolase,through screening the transcriptome of *** under water loss *** expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia *** NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on½Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling *** analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related ***,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H_(2)O_(2))and soil drought treatment at the seedling *** traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG ***,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT *** two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton ***,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.

关键词： antioxidant enzyme β-carotene ketolase drought resistance nostoc flagelliforme osmotic stress rice transcriptome analysis