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Small RNA sequencing revealed aberrant piRNA expression profiles in deciduas of recurrent spontaneous abortion patients

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BIOCELL 2022年第4期46卷 1013-1023页

作者： JIABAO WU XIAOHUA LIU LU HAN HUA NIE YUAN TANG YUNGE TANG GE SONG LIXIN ZHENG WEIBING QIN NHC Key Laboratory of Male Reproduction and Genetics Guangzhou510600China Department of Center Laboratory Provincial Reproductive Science Institute(Guangdong Provincial Fertility Hospital)Guangzhou510600China Human Sperm Bank of Guangdong Province Guangzhou510600China Department of Reproduction Immunology and Genetics Provincial Reproductive Science Institute(Guangdong Provincial Fertility Hospital)Guangzhou510600China Department of Reproduction Medicine Provincial Reproductive Science Institute(Guangdong Provincial Fertility Hospital)Guangzhou510600China

Piwi-interacting RNAs(piRNAs)is a novel class of non-coding RNAs.However,changes in piRNA expression profiles in recurrent spontaneous abortion(RSA)have not yet been investigated.The aim of this study was to identify differentially expressed piRNAs in deciduas of RSA patients.Decidua tissues were collected by curettage from recruited RSA patients and normal early pregnant(NEP)women with their informed consent.Small RNA sequencing was used to evaluate the differences in piRNA expression profiles between RSA and NEP.The present results demonstrated that the counts of total piRNA reads in RSA samples were increased compared with those in NEP samples(0.21%vs.0.11%).Differential expression analysis identified 29 upregulated piRNAs and 18 downregulated piRNAs in RSA samples.RT-qPCR further confirmed that the expression levels of uniq-109625,uniq-89328,uniq-50651 and uniq-4569 were decreased in 8 RSA tissues,compared with 13 NEP tissues.Otherwise,pi-22628 and uniq-173406 were increased in 8 RSA tissues.Based on GO term and KEGG pathway analysis,we speculate that these piRNAs regulate RSA by targeting extracellular matrix component pathway,cell adhesion pathway and focal adhesion pathway.PiRNAs may be involved in RSA pathogenesis by target genes function on adhesion and extracellular matrix component.

关键词： Recurrent spontaneous abortion normal early pregnancy piRNA RNA sequencing Expression profile

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Potential role of circulating micro RNAs as a biomarker for unexplained recurrent spontaneous abortion

Potential role of circulating micro RNAs as a biomarker for ...

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第十届全国免疫学学术大会

作者： Weibing Qin Yunge Tang Ning Yang Rong Cui Jiehua Wu Key Laboratory of Male Reproductive and Genetics National Health and Family Planning Commission Department of Male Reproduction Family Planning Research Institute of Guangdong

Objective:To comparatively analyze the circulating micro RNA(mi RNA)profiling between unexplained recurrent spontaneous abortion(URSA)and normal early pregnancies(NEP)by microarray,and to evaluate the potential role of circulating mi RNA as a biomarker for URSA.Methods:Samples of plasma were obtained from September 2012 to April 2013 from 38 women with spontaneous miscarriages and 38 normal pregnant women(control group)who underwent pregnancy termination at 72 weeks at the Department of Reproductive Immunology in Family Planning Special Hospital of Guangdong Province.The circulating micro RNA expression profile was assessed by microarray and realtime polymerase chain reaction analysis.Results:Twenty-five circulating micro RNAs were expressed differentially in URSA,compared with NEP.Of these,9circulating micro RNAs were overexpressed,and 16 circulating micro RNAs were downexpressed.Four differentially expressed circulating mi RNAs(mi R-320b,mi R-146b-5p,mi R-92a-3p,mi R-204-3p)were selected to validate the microarray results,real-time PCR data confirmed the relaiblity of microarray results.Thirteen differentially expressed circulating mi RNAs were chosen to validate their expression in other larger scale samples by real-time PCR.The further statistical analysis showed that four circulating mi RNAs(mi R-320b,mi R-146b-5p,mi R-221-3p,mi R-559)were up-reguated and one circulating mi RNA(mi R-101-3p)was down-regulated in URSA.The target genes of these differentially expressed circulating mi RNAs were predicted by mi Randa,mi Rbase and Targetscan,and we speculate these circulating mi RNAs regulating URSA by target genes function on immune,adhesion and angiogenesis.Conclusion:Taken together,these results suggest circulating micro RNAs provide a new promising diagnostic biomarker for URSA.

关键词： circulating micro RNA biomarker microarray unexplained recurrent spontaneous abortion normal early pregnancy

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