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norepinephrine transporter (NET) is expressed in cardiac sympathetic ganglia of adult rat

Cell Research 2001年第4期11卷 317-320页

作者： LI HE, SUN KAI MA , XIAO PING HU , GOU YUAN ZHANG , JIAN FEI Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China Cardiovascular Division of Changzheng Hospital, Shang Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Cardiovascular Division of Changzheng Hospital Shanghai China Pathological Division of Pu Nan Hospital Shanghai China

The sympathetic nervous system plays a cardinal role in regulating cardiac function through releasing the neurotransmitter norepinephrine (NE). In comparison with ceniral nervous system, the molecular mechanism of NE uptake in myocardium is not clear. In present study we proved that in rat the CNS type of NE transporter (NET) was also expressed in middle cervical-stellate ganglion complex (MC-SG complex) which is considered to control the activity of heart, but not expressed in myocardium. The results also showed that NET expression level in right ganglion was significantly higher than in the left, rendering the greater capacity of NE uptake in right ventricle, a fact which may contribute to the maintenance of right ventricular function under pathologic state.

关键词： norepinephrine transporter gene expression cardiac sympathetic ganglion, rat.

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The norepinephrine transporter gene is associated with the retardation symptoms of major depressive disorder in the Han Chinese population

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Neural Regeneration Research 2012年第25期7卷 1985-1991页

作者： Xinrong Li Ning Sun Yong Xu Yanfang Wang Suping Li Qiaorong Du Juyi Peng Jinxiu Luo Kerang Zhang Department of Psychiatry First Hospital of Shanxi Medical University Taiyuan 030001 Shanxi Province China Nursing College of Shanxi Medical University Taiyuan 030001 Shanxi Province China

The norepinephrine transporter plays an important role in the pathophysiology and pharmacological treatment of major depressive disorder. Consequently, the norepinephrine transporter gene is an attractive candidate in major depressive disorder research. In the present study, we evaluated the depression symptoms of subjects with major depressive disorder, who were all from the North of China and of Hart Chinese origin, using the Hamilton Depression Scale. We examined the relationship between two single nucleotide polymorphisms in the norepinephrine transporter, rs2242446 and rs5569, and the retardation symptoms of major depressive disorder using quantitative trait testing with the UNPHASED program, rs5569 was associated with depressed mood, and the GG genotype may be a risk factor for this; rs2242446 was associated with work and interest, and the TT genotype may be a risk factor for loss of interest. Our findings suggest that rs2242446 and rs5569 in the norepinephrine transporter gene are associated with the retardation symptoms of depression in the Hart Chinese population.

关键词： norepinephrine transporter major depressive disorder quantitative trait locus gene polymorphism retardation symptoms Hamilton Depression Scale endophenotype single nucleotidepolymorphism pathogenesis

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Cotransfecting norepinephrine transporter and vesicular monoamine transporter 2 genes for increased retention of metaiodobenzylguanidine labeled with iodine 131 in malignant hepatocarcinoma cells

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Frontiers of Medicine 2017年第1期11卷 120-128页

作者： Yanlin Zhao Xiao Zhong Xiaohong Ou Huawei Cai Xiaoai Wu Rui Huang Department of Nuclear Medicine West China Hospital Sichuan University Chengdu 610041 China

norepinephrine transporter （NET） transfection leads to significant uptake of iodine-131-labeled metaiodobenzylguanidine （^131I-MIBG） in non-neuroendocrine tumors. However, the use of ^131I-MIBG is limited by its short retention time in target cells. To prolong the retention of ^131I-MIBG in target cells, we infected hepatocarcinoma （HepG2） cells with Lentivirus-encoding human NET and vesicular monoamine transporter 2 （VMAT2） genes to obtain NET-expressing, NET-VMAT2-coexpressing, and negative-control cell lines. We evaluated the uptake and efflux of 131I-MIBG both in vitro and in vivo in mice bearing transfected tumors. NET- expressing and NET-VMAT2-coexpressing cells respectively showed 2.24 and 2.22 times higher ^131I-MIBG uptake than controls. Two hours after removal of ^131I-MIBG-containing medium, 25.4% efflux was observed in NET- VMAT2-coexpressing cells and 38.6% in NET-expressing cells. In vivo experiments were performed in nude mice bearing transfected tumors; results revealed that NET-VMAT2-coexpressing tumors had longer ^131I-MIBG retention time than NET-expressing tumors. Meanwhile, NET-VMAT2-coexpressing and NET-expressing tumors displayed 0.54% and 0.19%, respectively, of the injected dose per gram of tissue 24 h after ^131I-MIBG administration. Cotransfection of HepG2 cells with NET and VMAT2 resulted in increased ^131I-MIBG uptake and retention. However, the degree of increase was insufficient to be therapeutically effective in target cells.

关键词： norepinephrine transporter vesicular monoamine transporter 2 ^131I-MIBG gene therapy lentivims vector

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Identification of Ser^(354) and Ser^(357) involved in the function of norepinephrine transporter

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Chinese Science Bulletin 1998年第18期43卷 1541-1544页

作者： HUANG Fang LIU Yanhong FEI Jian GUO Lihe Wolfgang Schwarz Shanghai Institute of Cell Biology Chinese Academy of SciencesShanghai 200031China Max-Planck Institute for Biophysik Kennedy Allee 7060956 Frankfurt/MainGermany

Conserved Ser 354, Ser 357 in the seventh transmembrane domain of NET were mutated and the function of mutants was studied by 3H-NE influx measurement. Double mutation at these two serine sites reduced the activity of NET to base level. It is inferred that these two sites may be involved in the recognition and transport activity of the NET protein.

关键词： norepinephrine transporter mutants.

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