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Survival of encapsulated probiotics through spray drying and non-refrigerated storage for animal feeds application

Agricultural Sciences 2013年第5期4卷 78-83页

作者： Norfahana Abd-Talib Siti Hamidah Mohd-Setapar Aidee Kamal Khamis Lee Nian-Yian Ramlan Aziz 1Institute Bio Product Development Universiti Teknologi Malaysia UTM Johor Bahru Johor Malaysia 2Centre of Lipid Engineering and Applied Research (CLEAR) Universiti Teknologi Malaysia UTM Johor Bahru Johor Malaysia Centre of Lipid Engineering and Applied Research (CLEAR) Universiti Teknologi Malaysia UTM Johor Bahru Johor Malaysia Institute Bio Product Development Universiti Teknologi Malaysia UTM Johor Bahru Johor Malaysia

The objective of this study is to verify potential of various types of microorganisms during spray drying and non-refrigerated storage that can be enhanced substantially by selecting suitable protective colloids. Four selected probiotics tested are Lactbacillus plantarum B13 and B18, which are the bacteria probiotics and Kluyveromyces lactis and Saccharomyces blouradii, non-bacteria probiotics. Two levels of experiment occur starting with formulation study of encapsulation agent followed by the viability study of different probiotics after spray dry and two weeks nonrefrigerated storage. The formulation of 30% of gum Arabic, 15% of gelatin and 45% of coconut oil can homogenize well at least for two hours and can produce acceptable dried product (below 4% of moisture content) at low outlet temperature (70℃ -75℃). K. lactis, S. blouradii gives 2.57% and 2.4% of viability percentage after spray drying process and 25.84% and 2.04% after two weeks nonrefrigerated storage respectively. The colonies of non-probiotics bacteria after both conditions are between 1010 and 106 cfu/mL which is among the accepted level for industrial application. However, the survival of probiotics in a spray-dried form during non-refrigerated storage is higher at low of moisture content compared to others.

关键词： Probiotics Survival Rate Spray Drying nonrefrigerated Storage

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