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Risks involved in fecal DNA-based genotyping of microsatellite loci in the Amur tiger Panthera tigris altaica:a pilot study

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Journal of Forestry Research 2018年第2期29卷 517-523页

作者： Xue Zhang Yanchun Xu Hui Liu Guangshun Jiang Feline Research Center of Chinese State Forestry Administration College of Wildlife ResourcesNortheast Forestry UniversityHarbin 150040HeilongjiangPeople’s Republic of China Ocean and Fishery Bureau of Weifang Binhai Economic and Technological Development Zone Weifang 261108ShandongPeople’s Republic of China

In modern wildlife ecological research,feces is the most common non-invasive source of DNA obtained in the field and polymerase chain reaction(PCR) technology based on microsatellite markers is used to mine genetic information contained *** is especially the case for endangered ***,there are risks associated with this genotyping method because of the poor quality of fecal *** this study,we assessed genotyping risk across 12 microsatellite loci commonly used in previous tiger studies using blood and fecal DNA from captive Amur tigers(Panthera tigris altaica).To begin,we developed an index termed the accumulated matching rate of genotypes(R)between positive DNA(blood samples) and fecal DNA to explore the correct genotyping probability of a certain microsatellite *** found that different microsatelliteloci had different genotyping risks and required different PCR amplification *** genotyping errors we detected altered population genetic parameters and potentially impact subsequent *** on these findings,we recommend that:(1) four loci(E7,Fca094,Pti007 and Pti010) of 12 loci are not suitable for Amur tiger genetic research because of a low Rand difficulty reaching a stable status;(2) the Rof the 12 microsatellite loci plateaued differently,and considering limited budgets,amplification times of some loci could be increased when using fecal samples; and(3) future genetic analysis of wild Amur tigers should be corrected by genotyping error rates(1-R).

关键词： Amur tiger Genotyping risk Microsatellite non-invasive sample

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