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Expressions of Nucleoside Diphosphate Kinase (nm23) in Tumor Tissues are Related with Metastasis and Length of Survival of Patients with Hepatocellular Carcinoma

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Biomedical and Environmental Sciences 2010年第4期23卷 267-272页

作者： RUN AN JIE MENG QI SHI XIAO-XIA DAI JING-HONG CHEN YAN-JUN LEI BING SHAN CHEN GAO YONG-LIE CHU XIAO-PING DONG School of Medicine Xi'an Jiao-Tong University Xi'an 710061 Shanxi China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Ying-Xin Rd 100 Beijing 100052 China

Objective To evaluate the relationship of expressions of nucleoside diphosphate kinase （nm23） and proliferating cell nuclear antigen （PCNA）, as well as apoptosis, with the prognosis of HCC patients by analyzing their pathological and clinical data. Methods The expressions of nm23 and PCNA were analyzed by immunohistochemistry and the apoptotic phenomena were detected by TUNEL technique in the liver samples from 43 HCC tissues, 39 para-neoplastic tissues, and 10 normal tissues. The mean apoptosis index （AI） and proliferative index （PI） in individual sample were calculated. Results As shown by the detection, 32.6% of carcinomas had negative nm23 signal in tumor tissues, whereas all para-neoplastic and normal tissues had positive nm23. The AI in nm23 positive HCC was significantly higher than that in nm23 negative one, with statistical difference （P0.05）. Furthermore, the expressions of nm23, and the values of AI and PI were contrastively analyzed with some main pathological and clinical data of HCC. It revealed that HCC with extrahepatic metastasis showed remarkable correlation with the negative nm23 （P=0.013） and higher PI values of HCC （P=0.015）. The disease-free survival in HCC patients with negative nm23 expression was significantly poorer than that in patients with positive nm23 expression. Conclusion These data suggest that expressions of nm23 protein in tumor tissues are correlated with occurrences of metastasis and length of survival of the HCC patients, which may be an indicator for their prognosis.

关键词： Hepatocellular carcinoma nm23 PCNA Apoptosis Prognosis

Expression of nm23, KAI1 and spiral computed tomography findings in primary gallbladder carcinoma

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Chinese Medical Journal 2009年第21期122卷 2666-2668页

作者： JIANG Wen-xia SONG Bo-gen WANG Pei-jun Department of Pathology Medical College of Tongji University Shanghai 200092 China Department of Radiology Affiliated Tongji Hospital of Tongji University Shanghai 200065 China

Primary gallbladder carcinoma is the most common malignant tumor of the biliary system. There is a reduced likelihood for radical cure of primary gallbladder carcinomas because of the insidious onset.1 Spiral computed tomography （SCT） scanning is an important means for diagnosing lesions involving the gallbladder. We determined the expression of anti-metastasis protein nm23 and KAI1 using immunohistochemical method and analyzed the relationship between their expression and the pre-operatively SCT imaging findings, the histopathologic grade, clinical stage, 5-year survival rate in 35 cases of primary gallbladder cancer.

关键词： gallbladder carcinoma spiral computed tomography nm23 KAI1

nm23基因在头颈恶性肿瘤中表达的临床意义：META分析

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Nm23基因在头颈恶性肿瘤中表达的临床意义：META分析

作者：黄金晨湖北医药学院

学位级别：硕士

背景:癌症是一种具有较高死亡率的全球性疾病,其发病因素包括遗传背景因素、环境因素、生活习惯因素及病毒感染因素。头颈癌症已成为一种普遍存在的恶性疾病,在我国,它的发病率高达第九,而死亡率则排名第七。头颈恶性肿瘤患者死亡的主... 详细信息

背景:癌症是一种具有较高死亡率的全球性疾病,其发病因素包括遗传背景因素、环境因素、生活习惯因素及病毒感染因素。头颈癌症已成为一种普遍存在的恶性疾病,在我国,它的发病率高达第九,而死亡率则排名第七。头颈恶性肿瘤患者死亡的主要原因是癌细胞的远处转移。目前,肿瘤转移与基因多态性之间的关系已被多项研究报道。nm23基因阳性表达在抑制卵巢癌、结肠癌、肝癌等肿瘤远处转移的过程中发挥着重要作用。多项头颈部恶性肿瘤的研究表明,nm23基因在不同病理学分级、临床分期及是否出现局部复发中的表达情况不同。目的:应用Rev Man5.4进行META分析的方法,综合多个相关研究结果,分析nm23基因的表达与头颈部恶性肿瘤间的相关性,系统评价nm23基因对头颈部恶性肿瘤预后的影响,以期为临床医师评价及分析头颈肿瘤的预后提供循证医学证据。方法:计算机检索数据库:中国国家知识基础设施工程、万方数据库、维普中文科技期刊、Pub Med数据库、Web of Science、Embase。检索截至2022年12月发表的关于nm23在头颈部肿瘤中的表达及预后指标相关性的临床研究。纳入指标包括患者性别、年龄、临床T分期、淋巴结转移、M分期、临床Tnm分期、5年生存率、病理分化程度或级别、肿瘤复发等。严格按照纳入、排除标准筛选文献,对纳入文献的质量评价按照纽卡斯尔-渥太华评分量表（Newcastle-Ottawa scale,NOS）进行,应用Rev Man5.4软件对相关数据进行META分析,计算合并效应统计量比值比（Odds Ratio,OR）及95%可信区间（95%CI）,绘制森林图与漏斗图,使用Stata SE 16软件、切换随机/固定效应模型及逐一剔除个别报告后观察总体分析结果有无明显变化的方法作敏感性分析。结果:关于nm23基因与头颈肿瘤相关性研究共纳入86篇文献,其中鼻咽癌、喉癌、甲状腺癌相关文献数量分别为40、26、20篇。本研究结果表明,在鼻咽癌患者中,nm23基因阳性表达的患者处于T-T分期及Tnm分期Ⅰ-Ⅱ期的比率较nm23基因阴性表达患者大,nm23基因阳性的患者更不易发生淋巴结转移、远处转移、肿瘤复发（分别是OR=1.39,95%CI:1.07-1.80,P=0.01;OR=2.22,95%CI:1.38-3.57,P=0.001;OR=0.21,95%CI:0.13-0.34,Pnm23基因表达的差异无明显统计学意义;在喉癌患者中,nm23基因阳性的患者处于T-T期或疾病早期（Ⅰ-Ⅱ期）可能性大,较阴性患者淋巴结转移的概率低,其病灶病理组织学为高、中分化的可能性低分化大（分别是OR=2.01,95%CI:1.04-3.91,P=0.04;OR=4.47,95%CI:3.11-6.42,Pnm23基因阳性表达率在女性患者中更高,且nm23基因阳性的患者发生淋巴结转移的概率较阴性低、更多处于Tnm分期Ⅰ-Ⅱ期（分别是OR=0.60,95%CI:0.41-0.86,P=0.006;OR=0.29,95%CI:0.17-0.52,Pnm23基因表达情况与头颈肿瘤预后密切相关,是预测鼻咽癌、喉癌、甲状腺患者预后的重要因子,可能成为头颈肿瘤治疗的干预靶点,有利于临床个体化治疗方案的制定。

关键词： nm23 甲状腺癌喉癌鼻咽癌 META分析

NORTHERN BLOT ANALYSIS OF nm23 GENE EXPRESSION IN HUMAN LUNG CANCER

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Chinese Journal of Cancer Research 1999年第3期11卷 188-191页

作者：刘伦旭周清华石应康覃扬孙芝琳孙泽芳 Department of Thoracocardiac Surgery The First University Hospital West China University of Medical Sciences Chengdu China Department of Biochemistry School of Basic Medicine West China University of Medical Sciences Chengdu China

Objective: To investigate the role of nm23 gene expression in human lung cancer. Methods: Forty human lung cancer tissues and 19 non-cancer pulmonary tissues were studied for their nm23-H1 and nm23-H2 mRNA expression with non-radioactive Northern blot hybridization. The correlation of nm23 mRNA expression with clinical features of lung cancer was analyzed. Results: The mRNA expression of nm23-H2 gene in poorly differentiated squamous cell carcinoma was significantly decreased compared to that in moderate-high differentiated squamousd cell carcinoma. The mRNA expression of nm23-H1 and nm23-H2 gene in small cell lung cancer was significantly decreased compared to that in squamous cell carcinoma. No significant difference in nm23 mRNA expression was observed between lung cancer with and without lymph node metastasis, nor was there significant difference between tumor stage. Conclusion: The mRNA expression of nm23 gene is correlated with the degree of differentiation of lung cancer, but there is no evidence of metastasis suppression effect by nm23 gene.

关键词： Lung neoplasms nm23 Gene expression RNA Northern blotting

nm23、上皮型钙粘素和β-连环素在非小细胞肺癌中的表达及其与转移和预后的关系

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癌症 2005年第5期24卷 616-621页

作者：唐小军周清华张尚福刘伦旭四川大学华西医院四川省肺癌分子重点实验室四川成都610041 四川大学华西医院病理科四川成都610041 四川大学华西医院胸心血管外科四川成都610041

背景与目的:近年来关于nm23、上皮型钙粘素(E-cadherin)和连环素(Catenins)与肿瘤关系的研究比较多,但不同研究得到的结果差异较大。而且近年来的研究显示,nm23、E-cadherin和Catenins还参与细胞信号转导,使它们在肿瘤转移过程中的作用... 详细信息

背景与目的:近年来关于nm23、上皮型钙粘素(E-cadherin)和连环素(Catenins)与肿瘤关系的研究比较多,但不同研究得到的结果差异较大。而且近年来的研究显示,nm23、E-cadherin和Catenins还参与细胞信号转导,使它们在肿瘤转移过程中的作用可能具有多面性。本实验检测nm23、E-cadherin和β-Catenin在一组非小细胞肺癌(non-sm allcelllang cancer,NSCLC)中的表达水平,试图了解它们与非小细胞肺癌转移和预后的关系,以及它们之间的相互关系。方法:采用免疫组化(SP法)检测112例NSCLC和30例肺良性病变组织中nm23、E-cadherin和β-Catenin的表达水平。结果:(1)nm23、E-cadherin和β-Catenin在肺癌组织中表达水平(分别为53.0%,53.1%和47.2%)显著低于癌旁肺组织中的表达水平(分别为64.8%,79.7%和80.6%)和肺良性病变组织中的表达水平(分别为76.9%,83.5%和85.6%);(2)nm23、E-cadherin和β-Catenin在有淋巴结转移的肺癌组织中的表达水平(分别为48.0%,47.3%和41.2%)显著低于无淋巴结转移者(分别为65.0%,60.5%和60.3%),在Ⅲ期和Ⅳ期NSCLC的表达水平(分别为44.8%,46.6%和38.1%)均显著低于Ⅰ期、Ⅱ期NSCLC(分别为67.2%,64.3%和63.1%);(3)nm23、E-cadherin和β-Catenin高表达组5年生存率(分别为39.29%,35.85%和37.29%)

关键词：肺肿瘤 nm23 钙粘素连环素免疫组化

nm23 expression in gastric carcinoma and its relationship with lymphoproliferation

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World Journal of Gastroenterology 1999年第1期5卷 92-94页

作者： WANG Yang Kun 1, JI Xiao Long 2 and MA Nai Xu 1

Ｔｕｍｏｒｓｐｒｅａｄｉｓａｃｏｍｐｌｅｘｂｉｏｌｏｇｉｃａｌｐｒｏｃｅｓｃｌｏｓｅｌｙｒｅｌａｔｅｄｔｏｔｕｍｏｒｇｒｏｗｔｈ，ｗｈｉｃｈｉｓｒｅｇｕｌａｔｅｄｂｙｍａｎｙｇｅｎｅｓｗｉｔｈｉｎｔｈｅｃｅｌ．Ｒｅｃ... 详细信息

Ｔｕｍｏｒｓｐｒｅａｄｉｓａｃｏｍｐｌｅｘｂｉｏｌｏｇｉｃａｌｐｒｏｃｅｓｃｌｏｓｅｌｙｒｅｌａｔｅｄｔｏｔｕｍｏｒｇｒｏｗｔｈ，ｗｈｉｃｈｉｓｒｅｇｕｌａｔｅｄｂｙｍａｎｙｇｅｎｅｓｗｉｔｈｉｎｔｈｅｃｅｌ．Ｒｅｃｅｎｔｓｔｕｄｉｅｓｈａｖｅｒｅ?..

关键词： stomach neoplasms nm23 protein gene expression lymphoproliferation lymphocytes

nm23-H1基因表达与卵巢癌转移的相关性(英文)

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癌症 2004年第6期23卷 650-654页

作者：高庆蕾马丁孟力王世宣王常玉卢运萍张阿丽李静华中科技大学同济医学院附属同济医院妇产科湖北武汉430030 美国西南医学中心

背景与目的:转移是卵巢癌治疗失败及患者死亡的首要原因。然而,目前对卵巢癌转移潜能的分子机制知之不多。本研究旨在筛选高频转移卵巢恶性肿瘤细胞,分析卵巢癌高频转移细胞模型中nm23-H1基因表达与肿瘤转移特性的相关性,为系统实验研... 详细信息

背景与目的:转移是卵巢癌治疗失败及患者死亡的首要原因。然而,目前对卵巢癌转移潜能的分子机制知之不多。本研究旨在筛选高频转移卵巢恶性肿瘤细胞,分析卵巢癌高频转移细胞模型中nm23-H1基因表达与肿瘤转移特性的相关性,为系统实验研究和临床实践提供依据。方法:通过反复动物接种和体外培养,观察动物肺转移状况,筛选高频转移细胞株,比较原发肿瘤和转移肿瘤的特征,并应用Northernblot和Westernblot方法测定各类肿瘤细胞nm23mRNA和蛋白表达水平。结果:8株卵巢恶性肿瘤细胞中,4株有较高转移潜能,多次培养接种可筛选出高频转移细胞亚群。各类细胞nm23mRNA和蛋白表达水平与肿瘤转移特性呈负相关(r=0.96,P=0.0001)。结论:由基因分子水平决定的肿瘤转移趋势在不同肿瘤种类及细胞亚群中有明显差异;卵巢癌中nm23mRNA和蛋白的表达与其转移能力的降低有密切关系,可作为判定卵巢癌预后的敏感指标。

关键词：卵巢肿瘤转移 nm23 预后

精子相关抗原6和nm23蛋白在人骨肉瘤中的表达及临床意义

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精子相关抗原6和NM23蛋白在人骨肉瘤中的表达及临床意义

作者：朱瑞直蚌埠医学院

学位级别：硕士

目的:探讨精子相关抗原6(Sperm-associated antigen 6,SPAG6)和肿瘤转移抑制因子23(non-metastasis23,nm23)蛋白在骨肉瘤分级中的表达和临床意义,为骨肉瘤的临床生物学指标和分子靶向治疗提供一定的理论指导。方法:1.收集蚌埠医学院第... 详细信息

目的:探讨精子相关抗原6(Sperm-associated antigen 6,SPAG6)和肿瘤转移抑制因子23(non-metastasis23,nm23)蛋白在骨肉瘤分级中的表达和临床意义,为骨肉瘤的临床生物学指标和分子靶向治疗提供一定的理论指导。方法:1.收集蚌埠医学院第一附属医院临床病理科2005年1月至2016年12月的临床资料,最终纳入了42例有完整临床病理资料的骨肉瘤患者作为实验组,12例骨软骨瘤患者作为对照组,对两组患者临床资料进行分析,并对实验组进行随访。2.采用免疫组织化学染色方法、q RT-PCR和Western blotting方法检测分析SPAG6和nm23在实验组和对照组中的表达水平,并分析其表达与患者临床病理资料及预后之间的联系。结果:1.在骨肉瘤组织中,SPAG6和nm23蛋白的阳性表达率分别为71.43%(30/42)和35.71%(15/42);在12例骨软骨瘤对照组标本中,SPAG6和nm23的阳性表达率分别为33.33%(4/12)和58.33%(7/12)。SPAG6在骨肉瘤组织中的阳性表达率显著高于对照组(Pnm23蛋白在骨肉瘤组织中的表达率显著低于骨软骨瘤对照组(Pnm23阳性表达率与远处转移(P=0.02)、病理分级(Pnm23的表达之间呈负相关(r=0.519,Pnm23阴性表达与患者预后呈负相关(Pnm23的阳性表达情况、病理分级、Enneking分期及远处转移是影响骨肉瘤患者预后的独立危险因素(Pnm23低表达与骨肉瘤患者的病理分级、转移、Enneking分期及预后呈负相关。2.SPAG6和nm23可作为骨肉瘤患者的预后生物标志物。

关键词：骨肉瘤 SPAG6 nm23 总体生存期预后

Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer

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World Journal of Gastroenterology 2005年第42期11卷 6601-6606页

作者： Yong-Jun Liu Pei-Song Yan Jun Li Jing-Fen Jia College of Environmental and MunicipalEngineering Xi'an University of Architecture and TechnologyXi'an 710055 Shaanxi Province China Department ot Pathology Xijing Hospital FourthMilitary Medical University Xi'an 710033 Shaanxi ProvinceChina Center of Basic Research Shaan'xiChaoying Biomedicine R&D Limited Company Xi'an 710061Shaanxi Province China College of Life Science North-west University Xi'an 710069 Shaanxi Province China

AIM: To investigate the relationship between the expression levels of nm23 mRNA, CD44s, and CD44v6,and oncogenesis, development and metastasis of human gastric adenocarcinoma, colorectal adenocarcinoma,intraductal carcinoma of breast, and lung cancer.METHODS: Using tissue microarray by immuhistochemical (IHC) staining and in situ hybri-dization (ISH), we examined the expression levels of nm23mRNA, CD44s, and CD44v6 in 62 specimens of human gastric adenocarcinoma and 62 specimens of colorectal adenocarcinoma; the expression of CD44s and CD44v6in 120 specimens of intraductal carcinoma of breast and 20 specimens of normal breast tissue; the expression of nm23 mRNA in 72 specimens of human lung cancer and 23 specimens of normal tissue adjacent to cancer.RESULTS: The expression of nm23 mRNA in the tissues of gastric and colorectal adenocarcinoma was not significantly different from that in the normal tissues adjacent to cancer (P＞0.05), and was not associated with the invasion of tumor and the pathology grade of adenocarcinoma (P＞0.05). However, the expression of nm23 mRNA was correlated negatively to the lymph node metastasis of gastric and colorectal adenocarcinoma (r = -0.49, P＜0.01; r = -4.93, P＜0.01). The expression of CD44s in the tissues of gastric and colorectal adenocarcinoma was significantly different from that in the normal tissues adjacent to cancer (P＜0.05;P＜0.01). CD44v6 was expressed in the tissues of gastric and colorectal adenocarcinoma only, the expression of CD44v6 was significantly associated with the lymph node metastasis, invasion and pathological grade of the tumor (r = 0.47, P＜0.01; r = 5.04, P＜0.01). CD44sand CD44v6 were expressed in intraductal carcinoma of breast, the expression of CD44s and CD44v6 was significantly associated with lymph node metastases and invasion (P＜0.01). However, neither of them was expressed in the normal breast tissue. In addition, the expression of CD44v6 was closely related to the degree of cell differentiation of intraductal carcinoma of breast (x2= 5.68, P＜0.05).

关键词： CD44s CD44v6 nm23 mRNA 基因表达肿瘤人类