检索结果-南通市图书馆

Selective and sensitive fluorescence imaging reveals microenvironment-dependent behavior of NO modulators in the endothelial system

引用

Journal of Pharmaceutical Analysis 2020年第5期10卷 466-472页

作者： Ying Dong Xiao-Rong Li Jia Li Yi Zang Xin Li College of Pharmaceutical Sciences Zhejiang UniversityHangzhou310058China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai201203China University of Chinese Academy of Sciences Beijing100049China Open Studio for Draggability Research of Marine Natural Products Pilot National Laboratory for Marine Science and Technology(Qingdao)1 Wenhai RoadAoshanweiJimoQingdao266237China

nitric oxide(NO)is a second messenger playing crucial roles in the signaling of a variety of cellular *** to its pathophysiological significance,various NO modulators have been developed to explore NO pathways and some have been used as *** modulators are often used directly to observe pharmacological effects in cell lines,but their actual effect on intracellular NO level is seldom ***,facilitated by a selective and sensitive fluorescence probe,we observed that some NO modulators displayed unexpected behaviors with both NO scavenger carboxy-PTIO and endothelial nitric oxide synthase(eNOS)inhibitor N(u)-nitro-L-arginine methyl ester(L-NAME)failing to decrease intracellular free NO level in ***926 cells while NO donor diethylamine-NONOate(DEA$NONOate)and eNOS activator calcimycin(A23187)failing to increase free NO level in human umbilical vein endothelial cell line(HUV-EC-C),although the reagents were confirmed to work normally in the primary human umbilical vein endothelial cells(primary HUVECs)and RAW 264.7 macrophage *** research suggested that these unusual behaviors might be attributed to the cellular microenvironments including both the NO synthase(NOS)level and the endogenous glutathione(GSH)*** manipulating eNOS level in both cells restores the expected response,while decreasing GSH level restores the ability of DEA$NONOate to increase NO level in *** results reveal that the cellular microenvironment has a profound impact on pharmacological *** study suggests GSH as a reservoir for NO in live cells and highlights the value of chemical probes as valuable tools to reveal microenvironmentdependent pharmacological effects.

关键词： nitric oxide modulator Drug screen Fluorescence imaging Microenvironment Endothelial cell

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：