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EFFECT OF RADiX SALViAE MiLTiORRHiZAE ON THE GENE EXPRESSiON OF nitric oxide synthase iN iSCHEMiC RAT BRAiNS

Journal of Traditional Chinese Medicine 1998年第2期18卷 128-133页

作者：吴卫平匡培根李振洲 DepartmentofNeurology ChinesePLAGeneralHospitalPostgraduateMilitaryMedicalSchoolBeijing.

The effect of Radix Salviae Miltiorrhizae (RSM) on the gene expression of nitric oxide synthase (NOS) in rat brains during ischemia was studied with in situ hybridization and the results were analyzed with iBAS 2000 image Analysis System. it was found that NOS gene expression of cerebral cortex and caudate-putamen was markedly increased in 24 hours in ischemia group (P

关键词： Animals Brain Brain ischemia Drugs, Chinese Herbal Gene Expression Male nitric oxide synthase nitric oxide synthase type i RNA, Messenger Random Allocation Rats Rats, Sprague-Dawley

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nitric oxide synthase gene expression in injured spinal cord tissue

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Chinese Medical Journal 2002年第5期115卷 740-742,154-155页

作者：刘成龙靳安民周初松陈斌第一军医大学珠江医院脊柱骨病科广州510282

OBJECTiVE: To investigate gene expression of three nitric oxide synthase isozymes in injured spinal cord tissue. METHODS: Thirty-six adult SD rats were randomly divided into six groups: a normal group and five injury groups, with six per each group. Animals in the injury groups were sacrificed at 2, 6, 12, 24, 48 h after injury. A compression injury model on the spinal cord was made according to Nystrom B et al and gene expression of the three NOS isozymes were examined by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Gene expression of nNOS and eNOS were detectable in the normal group and were up-regulated quickly after injury, reaching a maximum at 6 h: (0.633 +/- 0.012) and (1.236 +/- 0.207). Gene expression of iNOS was detectable only in the injury groups and it was gradually up-regulated after injury, reaching a maximum at 24 h: (1.043 +/- 0.049). CONCLUSiON: injury to the spinal cord leads to early up-regulation of cNOS and late up-regulation of iNOS. Different NOS isozymes may play different roles in secondary spinal cord injury.

关键词： Animals Female Gene Expression Regulation, Enzymologic Male nitric oxide synthase nitric oxide synthase type i nitric oxide synthase type ii nitric oxide synthase type iii RNA Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Spinal Cord Spinal Cord injuries

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Distribution of constitutive nitric oxide synthase in the jejunum of adult rat

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World Journal of Gastroenterology 2002年第3期8卷 537-539页

作者： ChenYM QianZM LifeScienceCollege HebeiNormalUniversityShijiazhuang050016HebeiProvinceChina DepartmentofAppliedBiologyandChemicalTechnology TheHongKongPolytechchnicUnioversityHungHomKowloonH

AiM: To study the distribution of the constitutive nitric oxide synthase (NOS) in the jejunum of adult rat. METHODS: The distribution of endothelial NOS (eNOS) was detected by immunohistochemistry. immunofluorescence histochemical dual staining technique were used for studying the distribution of neuronal NOS (nNOS) and eNOS. The dual stained slides were observed under a confocal laser scanning microscope. RESULTS: Positive neuronal NOS (nNOS) and endothelial NOS (eNOS) cells were found to be distributed in lamina propria of villi, and the epithelial cell was not stained. eNOS was mainly located in submucosal vascular endothelia, while nNOS was mainly situated in myenteric plexus. Some cells in the villi had both nNOS and eNOS. More than 80% of the cells were positive for both nNOS and eNOS, the rest cells were positive either for nNOS or for eNOS. CONCLUSiON: The two constitutive nitric oxide synthases are distributed differently in the jejunum of rat. nNOS distributed in myenteric plexus is a neurotransmitter in the non-adrenergic non-cholinergic (NANC) inhibitory nerves. eNOS distributed in endothelial and smooth muscle cells of blood vessels plays vasodilator role. eNOS and nNOS are coexpressed in some cells of lamina propria of villi. NO generated by those NOS is very important in the physiological and pathological process of small intestine.

关键词： Animals immunohistochemistry Jejunum Male nitric oxide synthase nitric oxide synthase type i nitric oxide synthase type iii Rats Rats, Sprague-Dawley Research Support, Non-U.S. Gov't Tissue Distribution

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