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Effects of different concentrations of nicotinamide on hematopoietic stem cells cultured in vitro

World Journal of Stem Cells 2024年第2期16卷 163-175页

作者： Yan Ren Yan-Ni Cui Hong-Wei Wang The Second Clinical Medical College Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology The Second Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology Key Laboratory of Molecular Diagnosis and Treatment of Blood Diseases in Shanxi ProvinceTaiyuan 030001Shanxi ProvinceChina

BACKGROUND In vitro expansion to increase numbers of hematopoietic stem cells(HSCs)in cord blood could improve clinical efficacy of this vital ***(NAM)can promote HSC expansion ex vivo,but its effect on hematopoietic stem and progenitor cells(HSPCs,CD34^(+)CD38)and functional subtypes of HSCs-shortterm repopulating HSCs(ST-HSCs,CD34^(+)CD38CD45RACD49f^(+))and long-term repopulating HSCs(LT-HSCs,CD34^(+)CD38CD45RACD49f^(+)CD90^(+))is not yet *** a sirtuin 1(SIRT1)inhibitor,NAM participates in regulating cell adhesion,polarity,migration,proliferation,and ***,SIRT1 exhibits dual effects by promoting or inhibiting differentiation in different tissues or *** propose that the concentration of NAM may influence proliferation,differentiation,and SIRT1 signaling of *** To evaluate the effects and underlying mechanisms of action of different concentrations of NAM on HSC proliferation and *** CD34^(+)cells were purified from umbilical cord blood using MacsCD34 beads,and cultured for 10-12 d in a serum-free medium supplemented with cytokines,with different concentrations of NAM added according to experimental *** cytometry was used to detect phenotype,cell cycle distribution,and apoptosis of the cultured ***-time polymerase chain reaction was used to detect the transcription levels of target genes encoding stemness-related factors,che mokines,components of hypoxia pathways,and antioxidant ***-dihydro-fluorescein diacetate probes were used to evaluate intracellular production of reactive oxygen species(ROS).Determination of the effect of different culture conditions on the balance of cytokine by cytometric bead *** Compared with the control group,the proportion and expansion folds of HSPCs(CD34^(+)CD38)incubated with 5 mmol/L or 10 mmol/L NAM were significantly increased(all P<0.05).The ST-HSCs ratio and fold expansion of the 5 mmol/L NAM group were significantly

关键词： Hematopoietic stem cells nicotinamide Concentration Proliferation Differentiation Sirtuin 1

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Transcriptome analysis shows nicotinamide seed treatment alters expression of genes involved in defense and epigenetic processes in roots of seedlings of Picea abies

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Journal of Forestry Research 2022年第4期33卷 1365-1375页

作者： Cecilia Laurell Torkel Berglund Anna B.Ohlsson Department of Industrial Biotechnology School of Engineering Sciences in ChemistryBiotechnology and Health(CBH)KTH Royal Institute of Technology10691 StockholmSweden

nicotinamide treatment of plants and plant cell cultures has been shown to promote defense and decrease levels of DNA *** the present study,we used RNA-seq technology to study overall changes in gene expression induced in roots of 3-month-old spruce(Picea abies)seedlings grown from nicotinamide-treated seeds to examine the molecular mechanisms underlying the defense *** 350 genes were identified as differentially expressed in roots after the seed *** response genes,including transcription factors MYB77 and LHY and two chitinase enzymes,were generally upregulated,whereas genes thought to be involved in epigenetic regulation such as DDM1,known to promote DNA methylation,were present at high frequency among the downregulated *** all samples,the expression of downregulated epigenetic-related genes was highly correlated with the nicotinamide treatment,indicating a common *** results support an earlier hypothesis regarding a potential role of nicotinamide as a defense-signal mediator.

关键词： DNA methylation Large-scale gene expression analysis nicotinamide Picea abies Seed treatment

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nicotinamide adenine dinucleotide phosphate oxidase activation and neuronal death after ischemic stroke

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Neural Regeneration Research 2019年第6期14卷 948-953页

作者： Jiamei Shen Radhika Rastogi Xiaokun Geng Yuchuan Ding China-America Institute of Neuroscience Beijing Luhe HospitalCapital Medical University Department of Neurosurgery Wayne State University School of Medicine Department of Neurology Beijing Luhe HospitalCapital Medical University

nicotinamide adenine dinucleotide phosphate oxidase(NOX) is a multisubunit enzyme complex that utilizes nicotinamide adenine dinucleotide phosphate to produce superoxide anions and other reactive oxygen species. Under normal circumstances, reactive oxygen species mediate a number of important cellular functions, including the facilitation of adaptive immunity. In pathogenic circumstances, however,excess reactive oxygen species generated by NOX promotes apoptotic cell death. In ischemic stroke, in particular, it has been shown that both NOX activation and derangements in glucose metabolism result in increased apoptosis. Moreover, recent studies have established that glucose, as a NOX substrate, plays a vital role in the pathogenesis of reperfusion injury. Thus, NOX inhibition has the potential to mitigate the deleterious impact of hyperglycemia on stroke. In this paper, we provide an overview of this research,coupled with a discussion of its implications for the development of NOX inhibition as a strategy for the treatment of ischemic stroke. Both inhibition using apocynin, as well as the prospect of developing more specific inhibitors based on what is now understood of the biology of NOX assembly and activation, will be highlighted in the course of our discussion.

关键词： nicotinamide adenine dinucleotide phosphate oxidase stroke nicotinamide adenine dinucleotide phosphate oxidase inhibitors reactive oxygen species ischemia/reperfusion neuroprotection hyperglycolysis NADPH NOX

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nicotinamide overload may play a role in the development of type 2 diabetes

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World Journal of Gastroenterology 2009年第45期15卷 5674-5684页

作者： Shi-Sheng Zhou Da Li Wu-Ping Sun Ming Guo Yong-Zhi Lun Yi-Ming Zhou Fu-Cheng Xiao Li-Xin Jing Shen-Xia Sun Li-Bin Zhang Ning Luo Fu-Ning Bian Wei Zou Lai-Bin Dong Zhi-Gang Zhao Sheng-Fan Li Xiao-Jie Gong Zeng-Guo Yu Chang-Bin Sun Cong-Long Zheng Dong-Ju Jiang Zheng-Ning Li Institute of Basic Medical Sciences Medical College Dalian University Dalian 116622 Liaoning Province China College of Environmental and Chemical Engineering -Dalian University Dalian 116622 Liaoning Province China Okazaki Institute for Integrative Bioscience National Institutes of Natural Sciences Okazaki 444-8787 Japan Department of Physiology Xinxiang Medical College Xinxiang 453003 Henan Province China Department of Life Sciences Liaoning Normal University Dalian 116029 Liaoning Province China - Department of Neurology PLA No. 91 HospitalJiaozuo 454003 Henan Province China Department of Medicine PLA No. 210 Hospital Dalian 116021 Liaoning Province China

AIM： To investigate whether nicotinamide overload plays a role in type 2 diabetes. METHODS： nicotinamide metabolic patterns of 14 diabetic and 14 non-diabetic subjects were compared using HPLC. Cumulative effects of nicotinamide and N^1-methylnicotinamide on glucose metabolism, plasma HzO2 levels and tissue nicotinamide adenine dinucleotide （NAD） contents of adult Sprague-Dawley rats were observed. The role of human sweat glands and rat skin in nicotinamide metabolism was investigated using sauna and burn injury, respectively. RESULTS： Diabetic subjects had significantly higher plasma N^1-methylnicotinamide levels 5 h after a 100-mg nicotinamide load than the non-diabetic subjects （0.89 ± 0.13 μmol/L vs 0.6 ± 0.13 μmol/L, P 〈 0.001）. Cumulative doses of nicotinamide （2 g/kg） significantly increased rat plasma Nl-methylnicotinamide concentrations associated with severe insulin resistance, which was mimicked by Nl-methy-Inicotinamide. Moreover, cumulative exposure to N^1- methylnicotinamide （2 g/kg） markedly reduced rat muscle and liver NAD contents and erythrocyte NAD/ NADH ratio, and increased plasma H2O2 levels. Decrease in NAD/NADH ratio and increase in H2O2 generation were also observed in human erythrocytes after exposure to N^1-methylnicotinamide in vitro. Sweating eliminated excessive nicotinamide （5.3-fold increase in sweat nicotinamide concentration 1 h after a 100-mg nicotinamide load）. Skin damage or aldehyde oxidase inhibition with tamoxifen or olanzapine, both being notorious for impairing glucose tolerance, delayed N^1- methylnicotinamide clearance. CONCLUSION： These findings suggest that nicotinamide overload, which induced an increase in plasma N^1- methylnicotinamide, associated with oxidative stress and insulin resistance, plays a role in type 2 diabetes.

关键词： Type 2 diabetes nicotinamide N^1-methyl-nicotinamide Insulin resistance Oxidative stress Liver Sweat glands

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nicotinamide-Induced Apoptosis Can Be Enhanced by Melatonin in Mouse Myeloma Cells

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Tsinghua Science and Technology 2006年第4期11卷 408-414页

作者：张贵友盛鸿智刘佳 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China College of Life Sciences Shandong Agricultural University Taian 271018 China

The mechanism of apoptosis induced by nicotinamide was investigated by treating mouse myeloma cells （Sp2/0） with various concentrations of nicotinamide. The typical hallmarks of apoptosis, including chromatin condensation and DNA fragmentation, were detected when cells were treated with nicotinamide at concentrations of 30, 40, 50, and 60 mmol/L. The apoptosis percentage increased with increasing nicotinamide concentration. Interestingly, the strong antioxidant melatonin did not restrain the apoptosis induced by nicotinamide in mouse myeloma cells but greatly increased the induction of nicotinamide on apoptosis. When cells were preincubated with 0.1, 1, and 10 mmol/L melatonin before nicotinamide induction, the percentage of apoptosis induced by 50 mmol/L nicotinamide markedly increased with increasing melatonin concentration. These results suggest that apoptosis induced by nicotinamide has no relationship with oxida tive stress and melatonin could enhance nicotinamide-induced apoptosis in mouse myeloma cells by stimulating cell division in a certain manner. nicotinamide may provide a new method to treat some kinds of tumors with no damage to normal tissues.

关键词： apoptosis nicotinamide melatonin mouse myeloma cells

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nicotinamide suppresses bevacizumab-induced epithelial-mesenchymal transition of ARPE-19 cells by attenuating oxidative stress

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International Journal of Ophthalmology(English edition) 2021年第4期14卷 481-488页

作者： Li Zhou De-Peng Shi Wen-Juan Chu Shan Song Xiang-Hui Hao Ling-Ling Yang Hai-Feng Xu Medical College Qingdao UniversityQingdao 266071Shandong ProvinceChina State Key Laboratory Cultivation Base Shandong Provincial Key Laboratory of OphthalmologyShandong Eye InstituteShandong First Medical University&Shandong Academy of Medical SciencesQingdao 266071Shandong ProvinceChina Qingdao Eye Hospital Shandong Eye InstituteShandong First Medical University&Shandong Academy of Medical SciencesQingdao 266071Shandong ProvinceChina

AIM:To investigate the effects of nicotinamide(NAM)on bevacizumab(BEV)-induced epithelial-mesenchymal transition(EMT)of human retinal pigment epithelial cells(ARPE-19)and the underling ***:ARPE-19 cells were treated with BEV for 24,48,and 72 h,and the variation degrees of EMTrelated markers(fibronectin,α-SMA,vimentin,and ZO-1)were assessed by Western blotting to select the optimal treatment time point which exhibited the most obvious changes of EMT-related markers for the subsequent ***,NAM was added to the medium,the m RNA and protein levels of the EMT-related markers were then *** accumulation of reactive oxygen species(ROS)and H_(2)O_(2) and the total antioxidant capacity(TAC)of the cells were also measured to evaluate the level of oxidative ***:After being treated with BEV for 72 h,the protein expression levels of EMT-related markers in ARPE-19 cells showed significant *** the levels of ROS and H_(2)O_(2) were obviously increased,and the TAC of ARPE-19 cells was *** 72 h was chosen to be the optimal treatment time point in ***,NAM inhibited BEV-induced EMT by downregulating fibronectin,α-SMA,and vimentin and upregulating ZO-1,decreased the accumulation of ROS and H_(2)O_(2),and enhanced TAC in BEV-treated ARPE-19 ***:This study demonstrates that NAM suppressed BEV-induced EMT in ARPE-19 cells by attenuating oxidative ***,NAM may be a potential therapeutic agent for alleviating neovascular fibrosis of the ocular fundus after anti-vascular endothelial growth factor therapy.

关键词： nicotinamide epithelial-mesenchymal transition bevacizumab oxidative stress ARPE-19 cells

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Effects of nicotinamide and riboflavin on the biodesulfurization activity of dibenzothiophene by Rhodococcus erythropolis USTB-03

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Journal of Environmental Sciences 2008年第5期20卷 613-618页

作者： YAN Hai SUN Xudong XU Qianqian MA Zhao XIAO Chengbin Ning Jun Department of Biological Science and Technology School of Applied Science University of Science and Technology Beijing Beijing 100083 China Research Center for Eco-environmental Science Chinese Academy of Sciences Beijing 100085 China

Rhodococcus erythropolis USTB-03 is a promising bacterial strain for the biodesulfurization of dibenzothiophene （DBT） via a sulfurspecific pathway in which DBT is converted to 2-hydroxybiphenyl （2HBP） as an end product. The effects of nicotinamide and riboflavin on the sulfur specific activity （SA） of DBT biodesulfurization by R. erythropolis USTB-03 were investigated. Both nicotinamide and riboflavin were found to enhance the expression of SA, which was not previously reported. When R. erythropolis USTB-03 was grown on a medium containing nicotinamide of 10.0 mmol or riboflavin of 50.0 μmol, SA was raised from 68.0 or so to more than 130 mmol 2HBP/（kg dry cells.h）. When R. erythropolis USTB-03 was grown in the presence of both nicotinamide of 5.0 mmol and riboflavin of 25.0 μmol, SA was further increased to 159.0 mmol 2HBP/（kg dry cells.h）. It is suggested that the biological synthesis of reduced form of flavin mononucleotide （FMNH2）, an essential coenzyme for the activities of biodesulfurization enzyme Dsz C and A, might be enhanced by nicotinamide and riboflavin, which was responsible for the increased SA of R. erythropolis USTB-03.

关键词： biodesulfurization dibenzothiophene nicotinamide Rhodococcus erythropolis USTB-03 riboflavin

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Synthesis and Crystal Structure of an Inclusion Compound Constructed through Self-assembly of nicotinamide and Dicyanamide Coordination Bridges

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Chinese Journal of Structural Chemistry 2010年第6期29卷 914-918页

作者：郑凌玲 College of Chemistry and Chemical Engineering Chongqing University

A novel inclusion compound of [Mn（dca）2（3-nic）2]n·（3-nic）2n（1,dca = dicyanamide,3-nic = nicotinamide） has been prepared and characterized by single-crystal X-ray diffraction,elemental analysis and *** complex crystallizes in the triclinic system,space group P1 with a = 7.5979（8）,b = 7.7128（9）,c = 14.5346（17）,α = 100.094（2）,β = 92.444（2）,γ = 116.736（2）o,V = 741.72（15） 3,Z = 1,Dc = 1.512 g/cm3,Mr = 675.55,μ = 0.507 mm-1,F（000） = 347,S = 1.023,the final R = 0.0400 and wR = *** inclusion compound is constructed by a three-dimensional host network that consists of dca,3-nic and Mn2＋ with coordination and hydrogen-bonding *** the network,one-dimensional rectangular channels are formed,and the guest molecules（3-nic） are included in the *** guest molecules interlink with the host through strong hydrogen bonds.

关键词： inclusion compound manganese(Ⅱ) dicyanamide nicotinamide

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Thermodynamics of Stability Constant of Binary Complex of nicotinamide with Mn^(++)

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Chinese Chemical Letters 2003年第11期14卷 1207-1210页

作者： Tuba SISMANOGLU Istanbul University Engineering Faculty Department of Chemistry34850Avcilar-IstanbulTurkey

The stability constants of the binary complexes of Mn2+ with nicotinamide (NA) were determined from potentiometric pH titrations data at 15.0 , 25.0 and 35.0oC and I = 0.1,0.2,0.4 mol L-1 (NaClO4). The formation of binary 1:1 , 1:2 NA-Mn complexes at three different temperatures and the influence of three different ionic strength on their stability were reported. The thermodynamic parameters (DGof, DSof, DHof) for the complex formation reaction were estimated from stability constant at different temperatures.

关键词： nicotinamide stability constant binary complexes.

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Effect of nicotinamide on newly diagnosed type 1 diabetic children

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Acta Pharmacologica Sinica 2006年第6期27卷 724-727页

作者： Madeeha KAMAL Abdul-JabbarAlABBASY AhoodAlMUSLEMANI Abdulbari BENER Department of Pediatrics Salmaniya Medical ComplexManamaBahrain School of Health Department of PediatricsHamad Medical CorporationDohaQatar Department of Pediatrics.Salmaniya Medical Complex ManamaBahrain Department of Medical Statistics and Epidemiology Hamad General HospitalHamad Medical CorporationDohaQatar Department of Evidence for Population Health Unit School of Epidemiology and Health SciencesUniversity of ManchesterManchester.UK

Aim:To determine whether a low dose of nicotinamide(NA)therapy for pediatricpatients with type 1 diabetes,initiated within the first 24 h of diagnosis,prolongsthe"honeymoon"period and lowers their insulin ***:Allchildren(n=66)with newly diagnosed type 1 diabetes admitted to Salmaniya MedicalComplex between 1998 and 2000,received NA 1-2 mg/kg per day,in addition tosc insulin *** patients were followed for 24 months(NA group).Findings inthis group were compared with records from a similarly diagnosed control group(n=37),who were admitted to the same hospital between 1995 and 1997 and did notreceive NA *** insulin dose per kg bodyweight required at baselineand at 3-monthly intervals up to 2 years after diagnosis was ***:At baseline,the two groups did not differ with respect to age,ethnic background,weight,insulin dose per kg bodyweight or glucose levels on ***,NA group had lower insulin requirements than control group at each 3-monthinterval up to 2 years after ***:Our study results suggest thateven low doses of oral NA given to children with newly diagnosed type 1 diabetesmay reduce insulin requirements and prolong the"honeymoon"period.

关键词： type 1 diabetes nicotinamide insulin therapy insulin dose glycated hemoglobin A

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