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Humoral immune responses in rabbits induced by an experimental inactivated severe acute respiratory syndrome coronavirus vaccine prepared from F69 strain

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Chinese Medical Journal 2004年第11期117卷 1625-1629页

作者：张传海郭中敏郑焕英陆家海王一飞鄢心革赵勇杜雄威张欣方苓凌文华戚树源余新炳钟南山 South China Institute of Botany Graduate School of Chinese Academy of Sciences Guangzhou 510650 China Department of Medical Statistics and Epidemiology School of Public Health Sun Yat-sen University Guangzhou 510080 China Center for Disease Control and Prevention of Guangdong Province Guangzhou 510300 China Biomedicine Research and Development Center Jinan University Guangzhou 510632 China Department of Medical Statistics and Epidemiology School of Public Health Sun Yat-sen University Guangzhou 510080 China Guangzhou Institute of Respiratory Diseases Guangzhou Medical College Guangzhou 510120 China

BACKGROUND: The etiologic agent of severe acute respiratory syndrome (SARS) has been confirmed to be a novel coronavirus (CoV), namely SARS-CoV. Developing safe and effective SARS-CoV vaccines is essential for us to prevent the possible reemergence of its epidemic. Previous experiences indicate that inactivated vaccine is conventional and more hopeful to be successfully developed. Immunogenicity evaluation of an experimental inactivated SARS-CoV vaccine in rabbits was conducted and reported in this paper. METHODS: The large-scale cultured SARS-CoV F69 strain was inactivated with 0.4% formaldehyde and purified, then used as the immunogen combined with Freund's adjuvant. Eight adult New Zealand rabbits were immunized four times with this experimental inactivated vaccine. Twelve sets of rabbit serum were sampled from the third day to the seventy-fourth day after the first vaccination. The titers of specific anti-SARS-CoV IgG antibody were determined by indirect enzyme-linked immunosorbent assay, and the neutralizing antibody titers were detected with micro-cytopathic effect neutralization test. RESULTS: Rapid and potent humoral immune responses were induced by the inactivated SARS-CoV vaccine in all the eight test rabbits. Titers of both specific IgG antibody and neutralizing antibody peaked at about six weeks after first vaccination, with the maximum value of 1:81 920 and 1:20 480, respectively. After that, serum antibody levels remained at a plateau or had a slight decrease, though two boosters were given in the succedent 4 to 5 weeks. Cross neutralization response existed between SARS-CoV F69 strain and Z2-Y3 strain. CONCLUSIONS: The inactivated SARS-CoV vaccine made from F69 strain owns strong immunogenicity, and the cross neutralization response between the two different SARS-CoV strains gives a hint of the similar neutralizing epitopes, which provide stable bases for the development of inactivated SARS-CoV vaccines.

关键词： Animals Antibodies, Viral Immunoglobulin G neutralization tests Rabbits Research Support, Non-U.S. Gov't SARS Virus Vaccines, Inactivated Viral Vaccines

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Serologic study on the outbreak of acute upper respiratory tract infections caused by adenovirus 3

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Frontiers of Medicine 2007年第2期1卷 215-218页

作者： JIANG Lufang JU Liwen JIANG Renjie LIN Yuzun ZHOU Liandi YU Shunzhang JIANG Qingwu Department of Health Microorganism School of Public HealthFudan UniversityShanghai 200032China Center for Disease Control and Prevention of Yancheng Yancheng 224000China

From April to June,2004,an outbreak of acute upper respiratory tract infections(AURTI)occurred in the north area of Jiangsu Province,*** throat swabs were collected with 13 of them presenting an adenovirus(Ad)-like cytopathogenic effect on *** were verified as Ad by the electron microscope,direct immunofluorescence assay and Ad primer-mediated ***,they were identified as adenovirus type 3(Ad3)by type-specific PCR and sequencing of the amplification *** serologic studies were carried out to finally diagnose and document the *** neutralization test of paired serum of six in nine cases show obviously increased antibodies *** positive rate of IgM,IgG and recovery phase neutralization antibodies of the cases were 3.7%,44.4%and 59.5%respectively while those of the controls were 0%,8.3%and 33.3%*** P values of Chi-Square were 0.510,0.018 and 0.226 *** concordance between IgG detected by ELISA and neutralization anti bodies detected by the neutralization test was 61.4%and the P value of Kappa was *** the serologic study,we can definitively diagnose that this outbreak of acute respiratory infections was caused by Adenovirus 3.

关键词： adenovirus infectious enzyme-linked immunosorbent assay immunoglobulin G neutralization tests

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