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neural circuits and temporal plasticity in hindlimb representation of rat primary somatosensory cortex:revisited by multi-electrode array on brain slices

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Neuroscience Bulletin 2010年第3期26卷 175-187页

作者：王丹丹李震常颖王蕊蕊陈雪峰赵振宇曹发乐金建慧刘明刚陈军首都医科大学疼痛生物医学研究所北京100069 第四军医大学唐都医院疼痛生物医学研究所暨功能性脑疾病研究所西安710038

Objective The well-established planar multi-electrode array recording technique was used to investigate neural circuits and temporal plasticity in the hindlimb representation of the rat primary somatosensory cortex （S1 area） . Methods Freshly dissociated acute brain slices of rats were subject to constant perfusion with oxygenated artificial cerebrospinal fluid （95% O2 and 5% CO2） , and were mounted on a Med64 probe （64 electrodes, 8×8 array） for simultaneous multi-site electrophysiological recordings. Current sources and sinks across all the 64 electrodes were transformed into two-dimensional current source density images by bilinear interpolation at each point of the 64 electrodes. Results The local intracortical connection, which is involved in mediation of downward information flow across layers II-VI, was identified by electrical stimulation （ES） at layers II-III. The thalamocortical connection, which is mainly involved in mediation of upward information flow across layers II-IV, was also characterized by ES at layer IV. The thalamocortical afferent projections were likely to make more synaptic contacts with S1 neurons than the intracortical connections did. Moreover, the S1 area was shown to be more easily activated and more intensively innervated by the thalamocortical afferent projections than by the intracortical connections. Finally, bursting conditioning stimulus （CS） applied within layer IV of the S1 area could success-fully induce long-term potentiation （LTP） in 5 of the 6 slices （83.3%） , while the same CS application at layers II-III induced no LTP in any of the 6 tested slices. Conclusion The rat hindlimb representation of S1 area is likely to have at least 2 patterns of neural circuits on brain slices： one is the intracortical circuit （ICC） formed by interlaminar connections from layers II-III, and the other is the thalamocortical circuit （TCC） mediated by afferent connections from layer IV. Besides, ICC of the S1 area is spatially limited, with les

关键词： planar multi-electrode array two-dimensional current source density imaging primary somatosensory cortex neural circuits long-term potentiation

Grand Research Plan for neural circuits of Emotion and Memory-Current status of neural circuit studies in China

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Neuroscience Bulletin 2013年第1期29卷 121-124页

作者： Yuan-Gui Zhu He-Qi Cao Er-Dan Dong Department of Health Sciences National Natural Science Foundation of China

During recent years, major advances have been made in neuroscience, i.e., asynchronous release, three-dimensional structural data sets, saliency maps, magnesium in brain research, and new functional roles of long non-coding RNAs. Especially, the development of optogenetic technology provides access to important information about relevant neural circuits by allowing the activation of specific neurons in awake mammals and directly observing the resulting behavior. The Grand Research Plan for neural circuits of Emotion and Memory was launched by the National Natural Science Foundation of China. It takes emotion and memory as its main objects, making the best use of cutting-edge technologies from medical science, life science and information science. In this paper, we outline the current status of neural circuit studies in China and the technologies and methodologies being applied, as well as studies related to the impairments of emotion and memory. In this phase, we are making efforts to repair the current deficiencies by making adjustments, mainly involving four aspects of core scientific issues to investigate these circuits at multiple levels. Five research directions have been taken to solve important scientific problems while the Grand Research Plan is implemented. Future research into this area will be multimodal, incorporating a range of methods and sciences into each project. Addressing these issues will ensure a bright future, major discoveries, and a higher level of treatment for all affected by debilitating brain illnesses.

关键词： neural circuits emotion and memory funding Grand Research Plan

Molecular and Anatomical Dissection of Olfactory neural circuits Using Transsynaptic PRV Tracers

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Molecular and Anatomical Dissection of Olfactory Neural Circ...

In vitro spatiotemporal imaging of biophotonic activities and transmission in neural circuits

中国神经科学学会第十二届全国学术会议

作者： Zhonghua Lu The Brain Cognition and Brain Disease Institute Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences

neural circuits mediating innate behavioral and physiological responses possess both molecular and anatomical features that ensure functional specificity towards distinct stimuli. Using newly developed retrograde monosynaptic-and polysynapticPseudorabies virus（PRV） tracers, we have searched for both molecular and anatomical characteristics of olfactory neural circuits that mediate innate reproductive or fear responses. We found small subsets of Olfactory Receptors（ORs） that send input to hypothalamic neurons controlling reproductive hormones. These receptors detect both pheromones and predator odors and may contribute to hormonal changes towards distinct chemical cues. Transsynaptic tracing from CRH neurons with PRV tracers identified a key area in the olfactory cortex that transmit predator odor information to mediate stress hormone reactions to predators.

关键词： neural circuits tracing PRV tracer circuit mapping

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In vitro spatiotemporal imaging of biophotonic activities an...

Activation of adult endogenous neurogenesis by a hyaluronic acid collagen gel containing basic fibroblast growth factor promotes remodeling and functional recovery of the injured cerebral cortex

中国神经科学学会第十届全国学术会议

作者： Rendong TANG Jiapei DAI Wuhan Institute for Neuroscience and Neuroengineering South-Central University for Nationalities

Objective The processing of neural information in neural circuits plays a key role in neural *** is well accepted that neuronal communication is mediated by bioelectricity and chemical molecules via the processes called bioelectrical and chemical transmission,respectively,which mainly occur in axons and ***,they seem to provide reasonable explanations for the basic functions and neural encoding of the nervous system,but many unanswered questions and debates *** has recently been suggested that biophotons,also called ultra-weak photon emissions,may play a potential role in neural signal transmission,contributing to the understanding of the high functions of nervous system;however,the experimental analysis of biophotonic activities(emissions) in neural circuits has been hampered due to technical *** By developing and optimizing an in vitro biophoton imaging method,we characterize the glutamate-induced biophotonic activities along the axons of cortical projection neurons and in the intrahippocampal circuits in mouse brain *** We show that the long-lasting application of 50 mM glutamate to brain slices produces a gradual and significant increase of biophotonic activities and achieves the maximal effect within approximately 90 min,which then lasts for a relatively long time(> 200 min).The initiation and/or maintenance of biophotonic activities by glutamate can be obviously blocked by oxygen and glucose deprivation,together with the application of a cytochrome c oxidase inhibitor(sodium azide),but only partly by an action potential inhibitor(TTX),an anesthetic(procaine),or the removal of intracellular and extracellular *** detected biophotonic activities mostly originate from axons,and the hyperphospholyation of microtubule-associated protein tau leads to a significant decrease of biophotonic activities in ***,the application of glutamate in the hippocampal dentate gyrus results in obvious biophotonic

关键词： neural circuits biophotons biophotonic transmission ultra-weak photon emission imaging system glutamate microtubule

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neural Regeneration Research 2025年第10期20卷 2923-2937页

作者： Yan Li Peng Hao Hongmei Duan Fei Hao Wen Zhao Yudan Gao Zhaoyang Yang Kwok-Fai So Xiaoguang Li Department of Neurobiology School of Basic Medical SciencesCapital Medical UniversityBeijingChina China Rehabilitation Science Institute China Rehabilitation Research CenterBeijing Key Laboratory of Neural Injury and RehabilitationBeijingChina Center of neural Injury and Repair Beijing Institute for Brain DisordersBeijingChina Beijing Key Laboratory for Biomaterials and neural Regeneration School of Engineering MedicineBeihang UniversityBeijingChina Guangdong-HongKong-Macao Institute of CNS Regeneration Ministry of Education CNS Regeneration Collaborative Joint LaboratoryJinan UniversityGuangzhouGuangdong ProvinceChina Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) GuangzhouGuangdong ProvinceChina Department of Ophthalmology and State Key Laboratory of Brain and Cognitive Sciences The University of Hong KongHong Kong Special Administration RegionChina Center for Brain Science and Brain-Inspired Intelligence Guangdong-HongKong-Macao Greater Bay AreaGuangzhouGuangdong ProvinceChina Co-innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Biomedical Engineering School of Biological Science and Medical EngineeringBeihang UniversityBeijingChina

The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after ***,whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions,such as the cortex,remains *** this study,we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic *** findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells,as well as their differentiation into mature and functionally integrated ***,these new neurons reconstructed the architecture of cortical layers II to VI,integrated into the existing neural circuitry,and ultimately led to improved brain *** findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries.

关键词： adult endogenous neurogenesis basic fibroblast growth factor-hyaluronic acid collagen gel cortical remodeling functional recovery migration motor cortex injury neural circuits neural stem cells newborn neurons proliferation

Brain circuits for retching-like behavior

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National Science Review 2024年第1期11卷 145-160页

作者： Lifang Huo Zhimin Ye Meiling Liu Ziqing He Meizhu Huang Dapeng Li Qian Wu Qian Wang Xiaoqun Wang Peng Cao Ji Dong Congping Shang School of Basic Medical Sciences Guangzhou National Laboratory The Fifth Afflliated Hospital Guangzhou Medical University Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Department of Neurobiology School of Basic Medical Sciences Beijing Key Laboratory of Neural Regeneration and Repair Advanced Innovation Center for Human Brain Protection Capital Medical University State Key Laboratory of Cognitive Neuroscience and Learning IDG/McGovern Institute for Brain Research Beijing Normal University Changping Life Science Laboratory National Institute of Biological Sciences

Nausea and vomiting are important defensive responses to cope with pathogens and toxins that invade the body. The nucleus of the solitary tract（NTS） is important for initiating these responses. However, the molecular heterogeneities and cellular diversities of the NTS occlude a better understanding of these defensive responses. Here, we constructed the single-nucleus transcriptomic atlas of NTS cells and found multiple populations of NTS neurons that may be involved in these defensive responses. Among these, we identified Calbindin1-positive（Calb1+） NTS neurons that are molecularly distinct from Tac1+*** Calb1+neurons are critical for nausea and retching induced by cereulide; an emetic toxin secreted by Bacillus Cereus. Strikingly, we found that cereulide can directly modulate vagal sensory neurons that innervate Calb1+NTS neurons, a novel mechanism distinct from that for nausea and retching induced by Staphylococcal enterotoxin A. Together, our transcriptomic atlas of NTS neurons and the functional analyses revealed the neural mechanism for cereulide-induced retching-like behavior. These results demonstrate the molecular and cellular complexities in the brain that underlie defensive responses to the diversities of pathogens and toxins.

关键词： vomiting nausea nucleus of the solitary tract Calbindin1 neural circuits

Input-output specific orchestration of aversive valence in lateral habenula during stress dynamics

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第12期25卷 1055-1065页

作者： Taida HUANG Xiaonan GUO Xiaomin HUANG Chenju YI Yihui CUI Yiyan DONG Department of Neurology and International Institutes of Medicine the Fourth Affiliated HospitalZhejiang University School of MedicineYiwu 322000China Department of Neurology of Sir Run Run Shaw Hospital and School of Brain Science and Brain Medicine Zhejiang University School of MedicineHangzhou 310058China NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Science and Brain-Machine IntegrationZhejiang UniversityHangzhou 310058China Research Centre the Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China Guangdong Provincial Key Laboratory of Brain Function and Disease Guangzhou 510080China Shenzhen Key Laboratory of Chinese Medicine Active Substance Screening and Translational Research Shenzhen 518107China

Stress has been considered as a major risk factor for depressive disorders,triggering depression onset via inducing persistent dysfunctions in specialized brain regions and neural *** various regions across the brain,the lateral habenula(LHb)serves as a critical hub for processing aversive information during the dynamic process of stress accumulation,thus having been implicated in the pathogenesis of *** neurons integrate aversive valence conveyed by distinct upstream inputs,many of which selectively innervate the medial part(LHbM)or lateral part(LHbL)of *** subregions also separately assign aversive valence via dissociable projections to the downstream targets in the midbrain which provides feedback *** these strides,the spatiotemporal dynamics of LHb-centric neural circuits remain elusive during the progression of depression-like state under *** this review,we attempt to describe a framework in which LHb orchestrates aversive valence via the input-output specific neuronal ***,a physiological form of Hebbian plasticity in LHb under multiple stressors has been unveiled to incubate neuronal hyperactivity in an input-specific manner,which causally encodes chronic stress experience and drives depression ***,the recent progress and future efforts in elucidating LHb circuits shed light on early interventions and circuit-specific antidepressant therapies.

关键词： Lateral habenula neural circuits Aversion Stress dynamics Depression-like state Head-to-head comparison

A Brief Summary of Current Therapeutic Strategies for Spinal Cord Injury

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Engineering 2022年第6期8卷 46-52页

作者： Chun Yao Xin Tang Yuqi Cao Xuhua Wang Bin Yu Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education&NMPA Key Laboratory for the Research and Evaluation of Tissue Engineering Technology Products Co-Innovation Center of NeuroregenerationNantong UniversityNantong 226001China NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Science and Brain-Machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhou 310003China Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantong UniversityNantong 226001China

Spinal cord injury(SCI)is a tremendous disaster in a person’s *** interrupts the brain-body neuronal circuits,resulting in functional *** of SCI is a progressive and comprehensive *** clinical trials,attempts to promote nerve regeneration and functional recovery after SCI have met with ***,with the development of transcriptome sequencing and biomaterials,researchers have struggled to explore novel efficient therapeutic treatments for ***,we summarize the recent pro-gress that has been made in SCI repair based on the lesion microenvironment,neural circuits,and bioma-terial *** also propose several important directions for future research,including targeted-microRNA therapy,blood vessel interventions,and multiple treatment *** short,we hope this review will enlighten researchers in the field and pave the way for SCI therapy.

关键词： Spinal cord injury Microenvironment neural circuits Biomaterial scaffolds

Prefrontal cortical circuits in anxiety and fear:an overview

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Frontiers of Medicine 2022年第4期16卷 518-539页

作者： Yihua Chen Nengyuan Hu Jianming Yang Tianming Gao State Key Laboratory of Organ Failure Research Key Laboratory of Mental Health of the Ministry of EducationGuangdong−Hong Kong−Macao Greater Bay Area Center for Brain Science and Brain-Inspired IntelligenceGuangdong Province Key Laboratory of Psychiatric DisordersDepartment of NeurobiologySchool of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China

Pathological anxiety is among the most difficult neuropsychiatric diseases to treat pharmacologically,and it represents a major societal *** have implicated structural changes within the prefrontal cortex(PFC)and functional changes in the communication of the PFC with distal brain structures in anxiety *** that affect the activity of the PFC,including cognitive therapies and transcranial magnetic stimulation,reverse anxiety-and fear-associated circuit abnormalities through mechanisms that remain largely *** the subjective experience of a rodent cannot be precisely determined,rodent models hold great promise in dissecting well-conserved *** developed genetic and viral tools and optogenetic and chemogenetic techniques have revealed the intricacies of neural circuits underlying anxiety and fear by allowing direct examination of hypotheses drawn from existing psychological *** review focuses on studies that have used these circuit-based approaches to gain a more detailed,more comprehensive,and more integrated view on how the PFC governs anxiety and fear and orchestrates adaptive defensive behaviors to hopefully provide a roadmap for the future development of therapies for pathological anxiety.

关键词： prefrontal cortex anxiety fear neural circuits optogenetics DREADD

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