检索结果-南通市图书馆

HSV-1 H129-Derived Anterograde neural circuit Tracers:Improvements, Production, and Applications

Neuroscience Bulletin 2021年第5期37卷 701-719页

作者： Hong Yang Feng Xiong Yi-Ge Song Hai-Fei Jiang Hai-Bin Qin Jing Zhou Sha Lu Steven FGrieco Xiangmin Xu Wen-Bo Zeng Fei Zhao Min-Hua Luo State Key Laboratory of Virology CAS Center for Excellence in Brain Science and Intelligence TechnologyCenter for Biosafety Mega-ScienceWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Shanghai Genechem Co.Ltd.Shanghai 201203 China Department of Anatomy and Neurobiology School of MedicineUniversity of CaliforniaIrvineCA 92697USA School of Basic Medical Sciences Capital Medical UniversityBeijing 100069China Chinese Institute for Brain Research Beijing 102206China Shanghai Public Health Clinical Center Fudan UniversityShanghai 200032China

Anterograde viral tracers are powerful and essential tools for dissecting the output targets of a brain region of interest. They have been developed from herpes simplex virus 1(HSV-1) strain H129(H129), and have been successfully applied to map diverse neural circuits.Initially, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dTK-tdT, which was highly successful, yet improvements are needed. Now, by inserting another tdTomato expression cassette into the H129-dTK-tdT genome, we have created H129-dTK-T2, an updated version of H129-dTK-tdT that has improved labeling intensity. To help scientists produce and apply our H129-derived viral tracers, here we provide the protocol describing our detailed and standardized procedures. Commonly-encountered technical problems and their solutions are also discussed in detail. Broadly, the dissemination of this protocol will greatly support scientists to apply these viral tracers on a large scale.

关键词： HSV-1 strain H129(H129) Anterograde transneuronal tracer Polysynaptic tracer Monosynaptic tracer Production Application neural circuit neural circuit tracing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

In Vivo Two-photon Calcium Imaging in Dendrites of Rabies Virus-labeled V1 Corticothalamic Neurons

引用

Neuroscience Bulletin 2020年第5期36卷 545-553页

作者： Yajie Tang Liang Li Leqiang Sun Jinsong Yu Zhe Hu Kaiqi Lian Gang Cao Jinxia Dai Academician Workstation of Animal Disease Control and Nutrition Immunity in Henan Province Henan Joint International Research Laboratory of Veterinary Biologies Research and ApplicationAnyang Institute of TechnologyAnyang 45000 China State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070China College of Veterinary Medicine Huazhong Agricultural UniversityWuhan 430070China Biomedical Center Huazhong Agricultural UniversityWuhan 430070China Key Laboratory of Development of Veterinary Diagnostic Products Ministry of AgricultureCollege of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China

Monitoring neuronal activity in vivo is critical to understanding the physiological or pathological functions of the brain.Two-photon Ca^(2+)imaging in vivo using a cranial window and specific neuronal labeling enables realtime,in situ,and long-term imaging of the living brain.Here,we constructed a recombinant rabies virus containing the Ca^(2+)indicator GCaMP6 s along with the fluorescent protein DsRed2 as a baseline reference to ensure GCaMP6 s signal reliability.This functional tracer was applied to retrogradely label specific V1-thalamus circuits and detect spontaneous Ca^(2+)activity in the dendrites of V1 corticothalamic neurons by in vivo two-photon Ca^(2+)imaging.Notably,we were able to record single-spine spontaneous Ca2+activity in specific circuits.Distinct spontaneous Ca^(2+)dynamics in dendrites of V1 corticothalamic neurons were found for different V1-thalamus circuits.Our method can be applied to monitor Ca^(2+)dynamics in specific input circuits in vivo,and contribute to functional studies of defined neural circuits and the dissection of functional circuit connections.

关键词： In vivo Ca^(2+)imaging Cranial window Two-photon microscopy Rabies virus Dendrite Primary visual cortex Corticothalamic projection neural circuit tracing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Illuminating neural circuits in Alzheimer’s Disease

引用

Neuroscience Bulletin 2021年第8期37卷 1203-1217页

作者： Yang Ying Jian-Zhi Wang Department of Pathophysiology School of Basic MedicineMinistry of Education Key Laboratory for Neurological DisordersHubei Key Laboratory for Neurological DisordersTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Alzheimer’s disease(AD)is the most common neurodegenerative disorder and there is currently no cure.neural circuit dysfunction is the fundamental mechanism underlying the learning and memory deficits in patients with AD.Therefore,it is important to understand the structural features and mechanisms underlying the deregulated circuits during AD progression,by which new tools for intervention can be developed.Here,we briefly summarize the most recently established cutting-edge experimental approaches and key techniques that enable neural circuit tracing and manipulation of their activity.We also discuss the advantages and limitations of these approaches.Finally,we review the applications of these techniques in the discovery of circuit mechanisms underlyingβ-amyloid and tau pathologies during AD progression,and as well as the strategies for targeted AD treatments.

关键词： neural circuit Alzheimer’s disease Single cell RNA sequencing neural circuit tracing Optogenetics Chemogenetics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：