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A rabies virus-based toolkit for efficient retrograde labeling and monosynaptic tracing

neural Regeneration Research 2023年第8期18卷 1827-1833页

作者： Kun-Zhang Lin Lei Li Wen-Yu Ma Xin Yang Zeng-Peng Han Neng-Song Luo Jie Wang Fu-Qiang Xu The Brain Cognition and Brain Disease Institute(BCBDI) Shenzhen Key Laboratory of Viral Vectors for BiomedicineShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsNMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal ProductsShenzhenKey Laboratory of Quality Control Technology for Virus-Based TherapeuticsGuangdong Provincial Medical Products AdministrationShenzhenGuangdong ProvinceChina State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in WuhanWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences-Wuhan National Laboratory for OptoelectronicsWuhanHubei ProvinceChina University of Chinese Academy of Sciences BeijingChina Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhanHubei ProvinceChina Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions ShenzhenGuangdong ProvinceChina Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghaiChina

Analyzing the structure and function of the brain's neural network is critical for identifying the working principles of the brain and the mechanisms of brain *** rabies viral vectors allow for the retrograde labeling of projection neurons and cell type-specific trans-monosynaptic tracing,making these vectors powerful candidates for the dissection of synaptic *** several attenuated rabies viral vectors have been developed,their application in studies of functional networks is hindered by the long preparation cycle and low yield of these *** overcome these limitations,we developed an improved production system for the rapid rescue and preparation of a high-titer CVS-N2c-ΔG *** results showed that the new CVS-N2c-ΔG-based toolkit performed remarkably:(1)N2cG-coated CVS-N2c-ΔG allowed for efficient retrograde access to projection neurons that were unaddressed by rAAV9-Retro,and the efficiency was six times higher than that of rAAV9-Retro;(2)the trans-monosynaptic efficiency of oG-mediated CVS-N2c-ΔG was 2–3 times higher than that of oG-mediated SAD-B19-ΔG;(3)CVS-N2c-ΔG could delivery modified genes for neural activity monitoring,and the time window during which this was maintained was 3 weeks;and(4)CVS-N2c-ΔG could express sufficient recombinases for efficient transgene *** findings demonstrate that new CVS-N2c-ΔG-based toolkit may serve as a versatile tool for structural and functional studies of neural circuits.

关键词： functional studies neural activity neural circuits projection neurons rAAV9-Retro rabies virus recombination retrograde labeling synaptic inputs trans-monosynaptic tracing

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Olfactory Decoding Method Using neural Spike Signals

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Journal of Measurement Science and Instrumentation 2010年第1期1卷 81-85页

作者： Kyung-jin YOU Hyun-chool SHIN Dept.of Electronic Engineering Soongsil UniversitySeoul 156-743Korea

This paper presents a novel method for inferring the odor based on neural activities observed from rats' main olfactory ***-channel extra-cellular single unit recordings are done by micro-wire electrodes(Tungsten,50 μm,32 channels)implanted in the mitral/tufted cell layers of the main olfactory bulb of the anesthetized rats to obtain neural responses to various *** responses as a key feature are measured by subtraction firing rates before stimulus from *** odor inference,a decoding method is developed based on the ML *** results show that the average decoding accuracy is about 100.0%,96.0%,and 80.0% with three rats,*** work has profound implications for a novel brain-machine interface system for odor inference.

关键词： olfactory odoronts inference neural decoding neural signal processing neural activity

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Simulation Study of the Dendritic Effect on Direct MRI Detection of neural Electric Event

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Journal of Electronic Science and Technology of China 2009年第1期7卷 92-95页

作者： Hong-Chuan Xiong Ying-Ling Huang Zhao-Tong Hu De-Zhong Yao Key Laboratory for Neurolnformation of Ministry of Education University of Electronic Science and Technology of China Chengdu 610054 China

Currently hemodynamic-based functional MRI technique is of limitation in temporal resolution. As neural activities in the brain accompany with current induced neuronal magnetic fields （NMF）, it is possible to utilize MRI to detect NMF directly thus to improve the temporal resolution. In this work, the contribution of dendrite branch to NMF is investigated by numeric simulation. The results indicate that the existence of dendrite branch may enhance the detectability of NMF by MRI directly.

关键词： Dendrite branch magnetic resonance imaging(MRI) neural activity neuronal magnetic field.

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Neuronal NMDA receptors regulate brain vascular development in zebrafish

Neuronal NMDA receptors regulate brain vascular development ...

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中国神经科学学会第十届全国学术会议

作者： Shan-Ye GU Ting-Ting LIU Rong-Wei ZHANG Qi CHEN Bing XU Jiu-Lin DU Institute of Neuroscience and State Key Laboratory of Neuroscience Shanghai Institutes for Biological SciencesChinese Academy of Sciences

Brain blood vasculature is critical for brain development and *** embryogenesis and early hfe,the nervous and vascular systems simultaneously develop and *** and how the nervous system regulates brain angiogenesis during development remain largely *** we reveal that neuronal NMDA receptors（NRs）can regulate brain vascular development via controlling VEGFA release in *** genetic downregulation or pharmacological blockade of NRs dramatically decreases brain angiogenesis during 4-7 days post-fertilization,meanwhile NR activation increases the *** effect requires neuronal NRs,because specific impairment of NRs in neurons but not endothelial cells leads to brain angiogenic defects,and the effect of global downregulation of NRs can be rescued by NR overexpression in *** neuronal NR action is mainly mediated by VEGFA,because NR downregulation reduces VEGFA synthesis and VEGFA overexpression in neurons can rescue the effect of NR ***,we find that neural activity can efficiently regulate brain angiogenesis mainly through ***,being detectors of neural activity,neuronal NRs can regulate brain vascular development via VEGFA signaling.

关键词： brain angiogenesis NMDA receptor VEGFA neural activity zebrafish

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Interaction between the actin cytoskeleton and the cadherin/catenin complex in regulating dendritic morphogenesis

Interaction between the actin cytoskeleton and the cadherin/...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Z.J TAN~(1,2),Y.YU~(1,2),Y.PENG~1,X YU~1 1 Institute of Neuroscience and Key Laboratory of Neurobiology,Shanghai Institute of Biological Sciences,Chinese Academy of Sciences,Shanghai,China 2 Graduate School of the Chinese Academy of Sciences,Shanghai 200031,China

＜正＞As dendrites are the primary sites through which neurons receive inputs from contacting axons,dendritic development and arborization are critical to neural circuit formation and synaptic *** have previously shown that the cadherin/catenin complex is critical for activity-dependent dendritic *** we further demonstrate that inter-neuronal cadherin-cadherin interactions play an important role in regulating dendritic arborization,as expression of the extracellular domain of N-cadherin in soluble form reduces dendritic arborization and blocks the effects of neural activity,as well as over-expression of beta-catenin or alphaN-catenin on increasing dendritic *** addition,we show the ability of alphaN-catenin to bind to the actin cytoskeleton is required for regulation of dendritic arbor development by the cadherin/catenin complex,an effect that cannot be mimicked by the equivalent domain of ***,we show using latrunculin A treatment that actin dynamics is required for enhancement but not maintenance of dendritic arbors.

关键词： dendritic arborization neural activity alphaN-catenin beta-catenin actin

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Oxytocin mediates early experience-dependent crossmodal plasticity in the sensory cortices

Oxytocin mediates early experience-dependent crossmodal plas...

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中国神经科学学会第十届全国学术会议

作者： Shu-Jing Li Jing-Jing Zheng Xiao-Di Zhang Wan-Ying Miao Xiang Yu Institute of Neuroscience and State Key Laboratory of Neuroscience Shanghai Institutes for Biological SciencesChinese Academy of Sciences University of Chinese Academy of Sciences

Objective During the early postnatal period,neural activity,both in the form of spontaneous electrical activity and natural sensory stimulation,is critical to the formation of functional neural *** addition to inducing changes within the target sensory cortex,sensory experience can also crossmodally affect other brain ***,little is known about the synaptic mechanisms underlying these crossmodal changes,especially during the first two weeks of postnatal development,at the peak of ***,we investigate the crossmodal effects of natural sensory experience during the first two postnatal weeks in the sensory *** We used whisker-deprivation,dark-rearing and enriched environment paradigms to increase or reduce neural activity,and examined their effects on synaptic transmission and neuronal excitability in layer 2/3 pyramidal neurons,as well as on the expression of various proteins in the somatosensory and visual *** We found a novel form of plasticity in neonatal mice,where early sensory experience crossmodally regulated development of all sensory ***,unimodal sensory deprivation from birth through whisker-deprivation or dark-rearing reduced excitatory synaptic transmission of layer2/3 pyramidal neurons both in the correspondent sensory cortex,and crossmodally in other sensory *** the other hand,increasing natural sensory experience through environment enrichment significantly increased excitatory synaptic transmission in both somatosensory and visual cortices,and also increased the protein level of post-synaptic proteins GluR2 and *** for changes in gene expression between normal and sensory deprived mice,we found that the mRNA level of the neuropeptide oxytocin secreted from the hypothalamus was significantly reduced in samples from sensory deprived ***,cortical oxytocin protein level was bidirectionally regulated by sensory ***,in vivo application of o

关键词： neural activity synaptic transmission sensory cortices crossmodal plasticity oxytocin

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