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Nestin expression and proliferation of ependymal cells in adult rat spinal cord after injury

Chinese Medical Journal 2002年第3期115卷 339-341页

作者：刘暌王忠诚王红云张亚卓北京市神经外科研究所北京100050

OBJECTIVE: To determine cell proliferation and nestin expression in the ependyma of adult rat spinal cord after injury. METHODS: Rat spinal cord injury models were established by aneurysm clip compression, and nestin expression and proliferation of ependymal cells at different times were shown with pathological and immuno-histochemical staining. RESULTS: Ependymal cells adjacent to the injured site demonstrated a dramatic increase in nestin expression 24 hours after compression. Proliferating cell nuclear antigen was positive, and significant proliferation was observed after 7 days. Nestin expression was down regulated as time went by. CONCLUSION: Normally quiescent mature ependymal cells appear to revert to an embryonic state in response to spinal cord injury.

关键词： nerve tissue proteins Animals Cell Division Ependyma Immunohistochemistry Intermediate Filament proteins Male Rats Rats, Wistar Spinal Cord Injuries

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Retinal dopamine transporter in experimental myopia

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Chinese Medical Journal 2002年第7期115卷 1027-1030,149页

作者：席晓勍褚仁远周行涛卢奕刘兴党复旦大学附属中山医院眼科上海200032 复旦大学附属眼耳鼻喉科医院眼科上海200031 复旦大学附属华山医院核医学科上海200031

OBJECTIVE: To investigate the distribution, changes and a possible role for retinal dopamine transporter (DAT) in experimental myopia in chickens. METHODS: Two-day-old chickens were divided into four groups. Chicken eyes were fitted with lenses of -10D,-20D and translucent goggles unilaterally. Normal eyes were used as controls. After 3 wk, all chickens were given an intramuscular injection of (125)I-beta-CIT 2beta-carbomethoxy-3beta-(4-iodophenyl)tropane and sacrificed two hours post injection. Retinal pigment epithelium (RPE) and the neural retina were obtained together or RPE was dissected out from the neural retina. Radioactive DAT from each specimen was assayed by gamma-counter. RESULTS: Retinal DAT was detected in RPE specimens rather than in the neural retina in all eyes. Radioactive DAT in myopic eyes was higher, compared with control eyes. CONCLUSIONS: Retinal DAT is mainly located in the RPE and may be involved in the formation of lens induced myopia (LIM) and form deprivation myopia (FDM). These methods may provide a new approach for further studying the role of the dopamine system in experimental myopia.

关键词： Membrane Glycoproteins nerve tissue proteins Animals Chickens Dopamine Plasma Membrane Transport proteins Eye Membrane Transport proteins Myopia Research Support, Non-U.S. Gov't Retina

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