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Tumor blood vessels formation in osteosarcoma:vasculogenesis mimicry

Chinese Medical Journal 2004年第1期117卷 94-98页

作者：蔡宣松贾永伟梅炯汤如勇 Department of Orthopedics Tongji HospitalTongji University Department of Pathology Tongji HospitalTongji University

BACKGROUND: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarcoma and to illustrate mechanism of tumor blood vessels formation in osteosarcoma. METHODS: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed with phase contrast microscope and transmission electron microscope (TEM). Morphometric studies using hematoxylin and eosin (HE) stain and CD31 immunohistochemical stain to show tumor-lined channels in human osteosarcoma were also performed. RESULTS: Observation with light microscope and TEM showed that highly aggressive osteosarcoma cell lines (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen, in the absence of endothelial cells or fibroblasts. Morphometric observation using HE stain and CD31 immunohistochemical stain showed that tumor cell-lined channels were also detected in vivo in osteosarcoma; by comparison, all vascular areas in the pedicle of osteochondroma or outside osteochondroma were endothelial-lined. CONCLUSION: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry.

关键词： Bone Neoplasms Cell Line, Tumor Collagen Humans Immunohistochemistry neovascularization, pathologic Osteosarcoma Research Support, Non-U.S. Gov't

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Effect of FGF-BP on angiogenesis in squamous cell carcinoma

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Chinese Medical Journal 2004年第4期117卷 621-623页

作者：李为民陈文彬 Division of Respiratory

Angiogenesis, the formation of new blood vessels, is a critical cellular process for cancer growth and *** targeting studies on overexpression and reduced expression of fibroblast growth factors binding protein (FGF-BP) have indicated that this protein plays a direct role in angiogenesis during tumor development. 1 To explore the effect of FGF-BP on neovascularization of squamous cell carcinoma, we investigated FGF-BP mRNA expression using in situ hybridization on squamous cell carcinoma. To further define the potential role of FGF-BP in individual stages of metastasis, we studied the association of FGF-BP with vascularity.

关键词： Adult Aged Aged, 80 and over Carcinoma, Squamous Cell Carrier Proteins Female Humans Male Middle Aged neovascularization, pathologic von Willebrand Factor

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