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Acta Pharmacologica Sinica 2005年第12期26卷 1485-1491页

作者： Chuan WANG Xiao-na DU Qing-zhong JIA Hai-lin ZHANG~2 Department of Pharmacology Hebei Medical University,Shijiazhuang 050017,China Department of Pharmacology Hebei Medical University Shijiazhuang China

Aim:To investigate the effects of the pleckstrin homology(PH)domain of phos-pholipase Cδ1(PLCδ1PH)on inhibition of phospholipase C(PLC)-mediated hy-drolysis of phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2]by ***:A fusion construct of green fluorescent protein(GFP)and PLCδ1PH(PLCδ1PH-GFP),which is known to bind PtdIns(4,5)P2specifically,together withlaser-scanning confocal microscopy,was used to trace PtdIns(4,5)***:Stimulation of the type 1 muscarinic receptor and the bradykinin 2 recep-tor induced a reversible PLCδ1PH-GFP translocation from the membrane to thecytosol in COS-7 *** inhibitor U73122 blocked the ***,a known PtdIns kinase inhibitor,did not affect the translocationinduced by ACh,but blocked recovery after translocation,indicating thatPtdIns(4,5)P2hydrolysis occurs through receptor-mediated PLC ***,a commonly used phospholipase C blocker,failed to block the recep-tor-induced PLCδ1PH-GFP translocation,indicating that neomycin is unable toblock PLC-mediated Ptdlns(4,5)Pe ***,in the absence of PLCδ1PH-GFP expression,neomycin abolished the receptor-induced hydrolysis ofPtdIns(4,5)P2 by ***:Although PLCδ1PH and neomycin bind toPtdIns(4,5)P2 in a similar way,they have distinct effects on receptor-mediatedactivation of PLC and PtdIns(4,5)P2 hydrolysis.

关键词： phosphatidylinositol 4,5-bisphosphate neomycin phospholipase C pleckstrin homology domains competitive binding acetylcholine green fluorescent proteins

Determination of neomycin in water samples by high performance anion chromatography with pulsed amperometric detection

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Chinese Chemical Letters 2007年第2期18卷 201-204页

作者： Bin Guan Dong Xing Yuan Environmental Science Research Center Xiamen University Xiamen 361005 China

A simple, fast and reliable method, using high performance anion chromatography with pulsed amperometric detection, had been developed for the analysis of neomycin in water samples. The elution and separation were carried out with an isocratic mobile phase, containing 10 mmol/L NaOH. The influence of the concentration and pH of the mobile phase on the separation and detection was investigated. A quadruple-potential waveform used for the detection was optimized. The detection limit of neomycin was down to 0.027 μg/mL. The linearity of neomycin calibration curve ranged from 0.050 to 0.505 μg/mL with correlation coefficient of 0.9997. R.S.D. (n = 11) was 4.0%.

关键词： neomycin High performance anion chromatography Pulsed amperometric detection

Synergistic Effect of Silver Nanoparticles with neomycin or Gentamicin Antibiotics on Mastitis-Causing Staphylococcus aureus

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Open Journal of Ecology 2016年第7期6卷 452-459页

作者： Shahab Jamaran Bahareh Rahimian Zarif Department of Biology Sanandaj Branch Islamic Azad University Sanandaj Iran

Objective: Mastitis is one of the most costly diseases in dairy cows, which greatly decreases milk production. Use of antibiotics in cattle leads to antibiotic-resistance of mastitis-causing bacteria. The present study aimed to investigate synergistic effect of silver nanoparticles (AgNPs) with neomycin or gentamicin antibiotic on mastitis-causing Staphylococcus aureus. Materials and Methods: In this study, 46 samples of milk were taken from the cows with clinical and subclinical mastitis during the august-October 2015 sampling period. In addition to biochemical tests, nuc gene amplification by PCR was used to identify strains of Staphylococcus aureus. Disk diffusion test and microdilution were performed to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Fractional Inhibitory Concentration (FIC) index was calculated to determine the interaction between a combination of AgNPs and each one of the antibiotics. Results: Twenty strains of Staphylococcus aureus were isolated from 46 milk samples and were confirmed by PCR. Based on disk diffusion test, 35%, 10% and 55% of the strains were respectively susceptible, moderately susceptible and resistant to gentamicin. In addition, 35%, 15% and 50% of the strains were respectively susceptible, moderately susceptible and resistant to neomycin. According to FIC index, gentamicin antibiotic and AgNPs had synergistic effects in 50% of the strains. Furthermore, neomycin antibiotic and AgNPs had synergistic effects in 45% of the strains. Conclusion: It could be concluded that a combination of AgNPs with either gentamicin or neomycin showed synergistic antibacterial properties in Staphylococcus aureus isolates from mastitis. In addition, some hypotheses were proposed to explain antimicrobial mechanism of the combination.

关键词： Staphylococcus aureus Mastitis Silver Nanoparticles Gentamicin neomycin Synergistic

Effects of 1-Butanol、neomycin and Calcium on Photosynthetic Characteristics of Transgenic Rice Plants Overexpressing Maize Phosphoenopyruvate Carboxylase

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Effects of 1-Butanol、Neomycin and Calcium on Photosynthetic...

Follicular contact dermatitis revisited: A review emphasizing neomycin-associated follicular contact dermatitis

中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Li Xia*, Wang Chao Institute of Foods Crop, Jiangsu Academy of Agriculture Sciences, Nanjing 210014,China *Corresponding author

＜正＞The maize gene encoding phosphoenolpymvate carboxylase（pepc）, one of the key enzyme in C4 photosynthetic pathway, has been introduced into C3 crop rice and high-level expression of the pepc gene was observed in

关键词： Rice PEPC 1-Butanol neomycin Photosynthesis Stomata

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World Journal of Clinical Cases 2014年第12期2卷 815-821页

作者： Philip R Cohen Division of Dermatology University of California San Diego San Diego CA 92131-3643 United States

Follicular contact dermatitis clinically presents as individual papules that include a central hair follicle. Pathologic features involve the follicle and the surrounding dermis: spongiosis and vesicle formation of the follicular epithelium associated with perifollicular and perivascular lymphocytic inflammation. Using the Pub Med database, an extensive literature search was performed on follicular contact dermatitis and neomycin. Relevant papers were reviewed and the clinical and pathologic features, the associated chemicals(including a more detailed description of neomycin), the hypothesized pathogenesis, and the management of follicular contact dermatitis were described. Several agentseither as allergens or irritants-have been reported to elicit follicular contact dermatitis. Several hypotheses have been suggested for the selective involvement of the follicles in follicular contact dermatitis: patient allergenicity, characteristics of the agent, vehicle containing the agent, application of the agent, and external factors. The differential diagnosis of follicular contact dermatitis includes not only recurrent infundibulofolliculitis, but also drug eruption, mite infestation, viral infection, and dermatoses that affect hair follicles. The primary therapeutic intervention for follicular contact dermatitis is withdrawal of the causative agent; treatment with a topical corticosteroid preparation may also promote resolution of the dermatitis. In conclusion, follicular contact dermatitis may be secondary to allergens or irritants; topical antibiotics, including neomycin, may cause this condition. Several factors may account for the selective involvement of the hair follicle in this condition. Treatment of the dermatitis requires withdrawal of the associated topical agent; in addition, topical corticosteroids may be helpful to promote resolution of lesions.

关键词： Allergic Contact Dermatitis Follicular Irritant neomycin Papular

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生物技术通讯 1994年第3期5卷 143-143页

作者：郭葆玉胡宗林徐惠敏孔宪涛陆德如第二军医大学医学生物技术和分子遗传研究所上海200433

腺相关病毒(AAV,Adeno associated Virus)是一类线状单股DNA的微小病毒。腺相关病毒因共具有定点整合(19q13 qter)及非病原性等特点而在基因转移与治疗中可望作为较为理想的载体。其在宿主染色体DNA上的整合需要辅助病毒共转染。根据Cav... 详细信息

腺相关病毒(AAV,Adeno associated Virus)是一类线状单股DNA的微小病毒。腺相关病毒因共具有定点整合(19q13 qter)及非病原性等特点而在基因转移与治疗中可望作为较为理想的载体。其在宿主染色体DNA上的整合需要辅助病毒共转染。根据Cavalier-Smith的线状SS DNA分子复制模式,即复制过程中3′OH端作为引物在DNA聚合酶的作用下合成另一姊妹股DNA分子,然后共价地连接到亲代未折叠的5′端回纹末端而进行DNA复制。

关键词：腺相关病毒 ITRs neomycin 染色体基因 DNA 末端反向重复折叠结构非病原性聚合酶辅助病毒

口服新霉素(neomycin)和土霉素(Terramycin)对肠内细菌的作用

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人民军医 1954年第7期 9-9页

作者：冯培

美奥诊所以前所进行的研究证明,金霉素和土霉素有排除肠内细菌的效力,但用药後却有抗药性的葡萄球菌形成,并引起局部和全身症状。这些抗药性的葡萄球菌对新霉素则有感受性,著者在本文介绍,新霉素在单独使用和与土霉素并用时,对肠内细菌... 详细信息

美奥诊所以前所进行的研究证明,金霉素和土霉素有排除肠内细菌的效力,但用药後却有抗药性的葡萄球菌形成,并引起局部和全身症状。这些抗药性的葡萄球菌对新霉素则有感受性,著者在本文介绍,新霉素在单独使用和与土霉素并用时,对肠内细菌的效力。在37名因各种肠管疾患而需行手术治疗的病人中,均予新霉素口服。其中34人在2—3日内,粪内便无需氧菌;而在其馀3人中,则分别有粪

关键词：葡萄球菌 neomycin Terramycin 需氧菌

Antibacterial Mechanisms of Berberine and Reasons for Little Resistance of Bacteria

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Chinese Herbal Medicines 2011年第1期3卷 27-35页

作者： JIN Jian-ling,HUA Guo-qiang,MENG Zhen,GAO Pei-ji State Key Laboratory of Microbial Technology,School of Life Sciences,Shandong University,Jinan 250100,China State Key Laboratory of Microbial Technology School of Life Sciences Shandong University Jinan 250100 China

Objective To study the antibacterial mechanisms of berberine and try to understand the reasons why bacteria cells difficultly resisted to *** Detecting the minimal inhibitory concentration(MIC) of bacterial cultures incubated under sub-MIC concentration of berberine,Huanglian,and neomycin for more than 200 generations,in order to analyze the bacteria *** the binding kinetics of berberine to DNA,RNA,and *** the changes in bacterial cell surface structure with scanning electron *** the Ca2 +and K +released from berberine-treated bacterial cells with atomic absorption *** the absorption of methyl-3H-thymine ( 3 H-dT),3 H-uridine( 3 H-U),and 3 H-tyrosine( 3 H-Tyr)into berberine-treated bacterial *** MICs of bacterial cultures,growing more than 200 generations in MH medium with 1/2 MIC of berberine(BA200) or Huanglian(HA200),did not increase compared to the control,while remarkably increased in MH medium with 1/2 MIC of neomycin (NA200).In addition,from the culture NA200 it was easy to isolate resistant mutant strains which could grow in MH medium with more than four times MIC neomycin,but from the culture BA200 and HA200 it was difficult to isolate berberine or Huanglian mutant strains could grow in MH medium with more than four times MIC berberine or *** binding kinetics of berberine to DNA,RNA,and proteins illustrated that berberine could easily and tightly bind to DNA and RNA,and hardly dis-bind from DNA-and RNA-berberine *** could easily bind to protein too,but also easily dis-bind from berberine-protein *** bacterial cells treated with berberine sharply decreased the absorption of 3 H-dT,3 H-U,and 3 H-Tyr,as the radioactive precursors of DNA,RNA,and protein *** could damage bacterial cell surface structure,especially for Gram-negative ***2 +and K + released from berberine-treated cells increased significantly compared to the

关键词： antibacterial effect antibiotic resistance berberine binding kinetics neomycin

A　study　on　the　integration　of　adeno－associated　virus　inverted　terminal　repeat　fold　structure　in　eukaryotic　chromosome　in　vitr

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Journal of Medical Colleges of PLA(China) 1994年第4期9卷 267-270页

作者：郭葆玉胡宗林徐慧敏孔宪涛陆德如

Ａｓｔｕｄｙｏｎｔｈｅｉｎｔｅｇｒａｔｉｏｎｏｆａｄｅｎｏ－ａｓｓｏｃｉａｔｅｄｖｉｒｕｓｉｎｖｅｒｔｅｄｔｅｒｍｉｎａｌｒｅｐｅａｔｆｏｌｄｓｔｒｕｃｔｕｒｅｉｎｅｕｋａｒｙｏｔｉｃｃｈｒｏｍｏｓｏｍｅｉｎｖｉｔｒ... 详细信息

Ａｓｔｕｄｙｏｎｔｈｅｉｎｔｅｇｒａｔｉｏｎｏｆａｄｅｎｏ－ａｓｓｏｃｉａｔｅｄｖｉｒｕｓｉｎｖｅｒｔｅｄｔｅｒｍｉｎａｌｒｅｐｅａｔｆｏｌｄｓｔｒｕｃｔｕｒｅｉｎｅｕｋａｒｙｏｔｉｃｃｈｒｏｍｏｓｏｍｅｉｎｖｉｔｒｏＧｕｏＢａｏｙｕ（郭葆玉）；Ｈｕ...

关键词： adeno-associated virus end-fold-ITRs neomycin resistance gene gene transduction