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Proteome Analysis of neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China

Biomedical and Environmental Sciences 2010年第4期23卷 251-258页

作者： YUAN HU ZHU-JUN SHAO XIAO-MEI YAN BO-QING LI FEI ZHAO DI XIAO JUN REN MING-HUAN ZHENG CHUN-XIANG FAN LI-HUA HE LI XU YI-XIN GU HAI JIANG FENG-HUA GUO ZHEN-WEI DAI HONG-YU REN MEI-JUAN LU XIA CHEN QING-HUA ZOU FAN-LIANG MENG JIAN-ZHONG ZHANG National Institute for Communicable Disease Control and Prevention China CDC P O Box 5 Changping Beijing 102206 China Anhui Provincial Center for Disease Control and Prevention Hefei 230061 Anhui China

Objective During 2003-2005, an outbreak of meningitis due to neisseria meningitidis serogroup C occurred in China. With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed. Methods Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3–10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. Results 502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426, with lower express volumes too. No Opa and Opc were detected in 053426. Conclusion The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.

关键词： neisseria meningitidis Reference map Two-dimensional gel electrophoresis MALDI-TOF/TOF-MS

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Molecular Characteristics of neisseria meningitidis Isolated during an Outbreak in a Jail: Association with the Spread and Distribution of ST-4821 Complex Serogroup C Clone in China

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Biomedical and Environmental Sciences 2013年第5期26卷 331-337页

作者： ZHANG Ji ZHOU Hai Jian XU Li HU Guang Chun ZHANG Xue Hua XU Sheng Ping LIU Zun Yu SHAO Zhu Jun Jinan Municipal Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention

Objective To characterize the meningococcal strains isolated from cases and close contacts with meningococcal disease associated with an outbreak in a jail in May 2010 by investigating the national distribution of hyperinvasive ST-4821 serogroup C clone associated with this outbreak. Methods The cases were described based on the clinical symptoms and laboratory results. Pharyngeal swabs were cultured for N. meningitidis from men in the jail. Meningococcal isolates were identified by serogrouping, pulsed-field gel electrophoresis （PFGE） and multilocus sequence typing （MLST）, respectively. Four hundred and sixteen serogroup C N. meningitidis strains were collected from 27 provinces between 2003 and 2010 for a nationwide survey and analyzed by PFGE and MLST. Results Three persons in a jail system were infected with invasive N. meningitidis serogroup C. All isolates tested had matching PFGE patterns and belonged to the multilocus sequence type （ST） 4821 clonal complex. All 47 N. meningitidis strains were identified from the pharyngeal swabs of 166 peoples in the jail, and 26 of them belonged to ST-4821 serogroup C clone, and 90.14% （375/416） serogroup C strains identified in the nationwide survey belonged to the ST-4821 complex. The ST-4821 serogroup C clone was spread nationwide, distributed in 24 provinces, especially in eastern provinces between 2003 and 2010. Conclusion Endemic transmission and carriage rate of ST-4821 serogroup C clone are high in this jail system. The ST-4821 serogroup C clone is spreading in China and nationwide distributed despite the existence of some effective vaccines.

关键词： Pulsed-field gel electrophoresis Multilocus sequence typing neisseria meningitidis ST-4821 Serogroup C

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Antimicrobial susceptibility and serotypes of neisseria meningitidis and Streptococcus pneumoniae in Sri Lanka:Experience from the National Reference Laboratory

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Asian Pacific Journal of Tropical Medicine 2022年第3期15卷 114-120页

作者： Lilani Karunanayake Veeraraghavan Balaji K.D.N.Gunawardana Rosemol Varghese Vayishnavi Ariram Malka Dassanayake Vaithehi Rajeevan Francis Lakmini Yapa Chamika Herath Nambage Shirani Chandrasiri Priyanka Wimalagunawardhana K.Dhananja Namalie C.T.Hapuarachchi Department of Bacteriology Medical Research InstituteColomboSri Lanka Department of Microbiology Christian Medical CollegeVelloreIndia Lady Ridgeway Children’s Hospital ColomboSri Lanka Department of Pathophysiology Faculty of Healthcare SciencesEastern UniversitySri Lanka Teaching Hospital AnuradhapuraSri Lanka District General Hospital MoneragalaSri Lanka Colombo South Teaching Hospital KalubowilaSri Lanka District General Hospital PolonnaruwaSri Lanka North Colombo Teaching Hospital RagamaSri Lanka District General Hospital MannarSri Lanka

Objective:To determine the antimicrobial susceptibility and serotypes of neisseria(N.)meningitidis and Streptococcus(S.)pneumoniae in Sri Lankan ***:We retrospectively analyzed 11 blood culture specimens from suspected patients with invasive meningococcal disease and 26 *** clinical *** tested 6 antimicrobials against *** and 12 antimicrobials against *** serogroup was determined by realtime PCR and Quellung serotyping was used for pneumococcal ***:*** serogroup B was the most common in this ***-susceptibility to penicillin was seen in 75.0%(6/8)of *** to ciprofloxacin,levofloxacin and cotrimoxazole was 62.5%(5/8),62.5%(5/8)and 87.5%(7/8),*** susceptibility was seen in cefotaxime and *** ***,the most common serotype was 19F in both invasive and non-invasive pneumococcal *** majority of strains showed multidrug *** non-susceptibility in non-meningeal strains were 13.6%and all meningeal strains were penicillin *** was highly resistant in both *** showed excellent susceptibility in non-invasive pneumococcal diseases ***,levofloxacin and vancomycin showed 100.0%susceptibility in all pneumococcal ***:Implementation of vaccines should be considered,especially for children and high-risk *** may contribute to reducing pneumococcal and meningococcal invasive disease burden and help prevent emergence of antimicrobial resistant strains.

关键词： neisseria meningitidis Streptococcus pneumoniae Sri Lanka Antimicrobial resistance Serotypes Invasive bacterial diseases

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A Novel Interpretation of Structural Dot Plots of Genomes Derived from the Analysis of Two Strains of neisseria meningitidis

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Genomics, Proteomics & Bioinformatics 2010年第3期8卷 159-169页

作者： Wilfred R. Cuff Venkata R.S.K. Duvvuri Binhua Liang Bhargavi Duvvuri Gillian E. Wu Jianhong Wu Raymond S.W. Tsang Public Health Agency of Canada Canadian Science Centre for Human and Animal Health Winnipeg Canada Center for Disease Modelling York Institute for Health Research York University Toronto Canada Department of Biology York University Toronto Canada Department of Medical Microbiology University of Manitoba Winnipeg Canada School of Kinesiology and Health Sciences York University Toronto Canada Department of Mathematics and Statistics York University Toronto Canada.

neisseria meningitidis is the agent of invasive meningococcal disease, including cerebral meningitis and septicemia. Because the diseases caused by different clonal groups （sequence types） have their own epidemiological characteristics, it is important to understand the differences among the genomes of the N. meningitidis clonal groups. To this end, a novel interpretation of a structural dot plot of genomes was devised and applied; exact nu- cleotide matches between the genomes ofN. meningitidis serogroup A strain Z2491 and serogroup B strain MC58 were identified, leading to the specification of various structural regions. Known and putative virulence genes for each N. meningitidis strain were then classified into these regions. We found that virulence genes of MC58 tend more to the translocated regions （chromosomal segments in new sequence contexts） than do those of Z2491, notably tending towards the interface between one of the translocated regions and the collinear region. Within the col- linear region, virulence genes tend to occur within 16 kb of gaps in the exact matches. Verification of these tendencies using genes clustered in the cps locus was sufficiently supportive to suggest that these tendencies can be used to focus the search for and understanding of virulence genes and mechanisms of pathogenicity in these two organisms.

关键词： structural dot plots virulence genes translocation match regions MUMmer neisseria meningitidis

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A first meningococcal meningitis case caused by serogroup X neisseria meningitidis strains in China

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Chinese Medical Journal 2008年第7期121卷 664-666页

作者： CHEN Chao ZHANG Tie-gang HE Jing-guo WU Jiang CHEN Li-juan LIU Jun-feng PANG Xing-huo YANG Jie SHAO Zhu-jun HUANG Ying-chun Laboratory of Plant Development Physiology and MolecularBiology College of Life Sciences Beijing Normal UniversityBeijing 100875 China Institu-te of Immunization and Prevention Beijing Center forDisease Prevention and Control Beijing 100013 China College of Life Science and Technology of Beijing University ofChemical Technology Beijing 100029 China National Institute for Communicable Disease Control andPrevention Chinese Center for Disease Control and PreventionBeijing 102206 China Department of Bio-pharmaceutical Science BiochemicalEngineering College of Beijing Union University Beijing 100023China

neisseria meningitidis is the leading cause of bacterial meningitis and classified into 13serogroups based on the immunological reactivity of the capsular polysaccharide.1 Serogroups A, B, C, W135 and Y are the most common causes of meningitis.2 Among them, serogroup A and C are the major causes of epidemics in Africa and Asia.2 Most of the epidemic outbreaks of meningococcal meningitis are caused by serogroup A neisseria meningitidis strain from the 1950s to the 1980s in China.3 During the years 2003 and 2005, a new sequence type （ST-4821） of serogroup C was identified in the Anhui and 11 other provinces of China.4

关键词： multilocus sequence typing neisseria meningitidis serogroup X

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Molecular characterizations of serogroup B neisseria meningitidis strains circulating in Beijing

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Chinese Medical Journal 2009年第5期122卷 584-587页

作者： ZHANG Tie-gang CHEN Chao HE Jing-guo WU Jiang CHEN Li-juan PANG Xing-huo YANG Jie SHAO Zhu-jun HUANG Ying-chun Institute of Immunization and Prevention Beijing Center for Disease Prevention and Control Beijing 100013 China College of Life Sciences Beijing Normal University Beijing 100875 China National Institute for Communicable Disease Control and Prevention China CDC Beijing 102206 China Department of Bio-pharmaceutical Science Biochemical Engineering College of Beijing Union University Beijing 100023 China

neisseria meningitidis （N. meningitidis） is classified into 13 serogroups based on the immunological reactivity of the capsular ***-s A,B and C are responsible for over 90% of meningococcal disease.2 In developed co... 详细信息

关键词： neisseria meningitidis outer membrane protein," pulsed-field gel electrophoresis serogroup B

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Preparation and Immunogenicity of Serogroup B Meningococcal OS-OMPC Conjugates

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Biomedical and Environmental Sciences 1999年第4期12卷 296-303页

作者： SUNYIN－YAN HUXU－JING InstituteofEpidemiologyandMicrobiology ChineseAcademyofPreventiveMedicineBeijing102206 InstituteofEp

The objective of the present study is to purify oligosaccharides (OSs) from serogroup B meningococci (3407 or 542852) with common lipooligosaccharide (LOS) immunotypes prevalent in China and to successtully conjugate them to their outer membrane protein complexes (OMPCs).Conjugates possessing broad cross-reactivity with different serogroup B meningococci were *** LOSs from the above two strains were purified on a Sephghex G75 column and hydrolyzed with acetic acid, and then the pare OSs were obtained by using a Sephadex G50 chromatographic column. The pure OSs were conjugated to corresponding OMPCs by carbodiimide mediated condensation. Mice were immunized with the OS-OMPC conjugates to produce antibodies. Immunognicities of the conjugates were tested by EUSA, bactericidal test and Western-blotting. The titers of antibodies against OS and LOS elicited by the two OS-OMPC conjugates increased respectively by 2. 5and 2 to 10 times as compared with those of antibodies against unconjugated OS and LOS. The sera elicited by the conjugates possessed a stronger bactericidal activity to the stndus (3407, 542852,29021) with the same LOS immunotype than to the strain with different LOS immunotype (86155).The results determined by ELISA indicated that the sera against the conjugaes showed strong response to the strains with the same or different LOS immunotypes. It was confirmed by westem-blotting analysis that the sera elicited by the two conjugates reacted with 42, 39 and 26 kDa proteins from the OMPC. Among the reactive bands, the 42kDa proteins were class 1 OMPs. The two serogroup B meningococcal OMPCs were strong immunogens and good carriers coupling with OSs as well. The immunogencity of the conjugaed OS was increased and the OS-OMPC conjugates of the serogroup B meogingococci showed a broad antigenic response to the strains tested.

关键词： Bacterial Outer Membrane Proteins Animals Antibodies Bacterial Blotting Western Enzyme-Linked Immunosorbent Assay Immunoconjugates Meningitis Meningococcal Mice neisseria meningitidis Oligosaccharides Research Support Non-U.S. Gov't

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Fatal meningococcal meningitis in a 2-year-old child:A case report

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World Journal of Clinical Cases 2019年第5期7卷 636-641页

作者： Aleksander Mularski Czesław Żaba Department of Legal and Forensic Medicine Poznan University of Medical Sciences Institute of Forensic Research

BACKGROUND In fatal cases of meningococcal septicemia, bacteriological diagnosis may not be straightforward due to postmortem replication and relocation of endogenic microflora. In medicolegal practice, aside from routine autopsy and histopathology, also other diagnostic methods, such as microbiological tests,immunohistochemistry and polymerase chain reaction(PCR), are used to examine body fluids and *** SUMMARY We present the case of sudden death in a 2-year-old child. The patient died approximately 30 min after hospital admission before any routine diagnostic procedures were undertaken. Presence of whole-body rash and fulminant course of the disease raised suspicion of meningococcal septicemia. An autopsy was performed seven days after death when the body showed the signs of late postmortem decomposition. No etiological factor of septicemia could be identified based on macro-and microscopic findings. However, PCR demonstrated the presence of genetic material of group W neisseria meningitidis in patient's cerebrospinal fluid and *** Microbiological PCR should be conducted postmortem whenever a specific etiological factor could not be identified with conventional methods.

关键词： Meningococcal infection Molecular microbiology Waterhouse-Friderichsen syndrome neisseria meningitidis Autopsy Cerebrospinal fluid Case report

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Meningococcal disease, a clinical and epidemiological review

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Asian Pacific Journal of Tropical Medicine 2017年第11期10卷 1019-1029页

作者： Rodrigo Siqueira Batista Andreia Patricia Gomes Jorge Luiz Dutra Gazineo Paulo Sergio Balbino Miguel Luiz Alberto Santana Lisa Oliveira Mauro Geller Laboratorio de Agentes Patogenicos Departamento de Medicina e Enfermagem Universidade Federal de VicosaVicosaMGBrazil Curso de Medicina Faculdade Dinamica do Vale do PirangaPonte NovaMGBrazil Hospital Universitario Clementino Fraga Filho Universidade Federal do Rio de JaneiroRio de JaneiroBrazil Curso de Medicina Centro Universitario Serra dos Orgaos (UNIFESO)TeresopolisRJBrazil School of Medicine New York University–NYUNew YorkUSA Departamento de Genetica Medica Universidade Federal do Rio de Janeiro (UFRJ)Rio de JaneiroRJBrazil

Meningococcal disease is the acute infection caused by neisseria meningitidis, which has humans as the only natural host. The disease is widespread around the globe and is known for its epidemical potential and high rates of lethality and morbidity. The highest number of cases of the disease is registered in the semi-arid regions of sub-Saharan Africa. In Brazil, it is endemic with occasional outbreaks, epidemics and sporadic cases occurring throughout the year, especially in the winter. The major epidemics of the disease occurred in Brazil in the 70's caused by serogroups A and C. Serogroups B, C and Y represent the majority of cases in Europe, the Americas and Australia. However, there has been a growing increase in serogroup W in some areas. The pathogen transmission happens for respiratory route(droplets) and clinically can lead to meningitis and sepsis(meningococcemia). The treatment is made with antimicrobial and supportive care. For successful prevention, we have some measures like vaccination, chemoprophylaxis and droplets' precautions. In this review, we have described and clarify clinical features of the disease caused by N. meningitidis regarding its relevance for healthcare professionals.

关键词： neisseria meningitidis Bacterial infection Meningococcal disease

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Optimal control and cost-effective analysis of the 2017 meningitis outbreak in Nigeria

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Infectious Disease Modelling 2019年第1期4卷 161-187页

作者： F.B.Agusto M.C.A.Leite Department of Ecology&Evolutionary Biology University of KansasLawrenceKSUnited States Mathematics and Statistics Unit University of South Florida at St.PetersburgFLUnited States

This paper presents a deterministic model for neisseria meningitidis,a bacterium that causes *** model was parameterized using data from the 2017 meningitis outbreak in *** control theory was applied to investigate the optimal strategy for curtailing the spread of the disease using control variables determined from sensitivity *** control variables are personal-protection such as the use of facial masks,and *** results show that the two controls avert more infections at low ***,a reciprocal relationship exists between the use of facial masks and *** is,when the use of facial masks is high,the use of vaccine is low and vice ***-effective analysis was applied to investigate the most cost-effective strategy from various combination of control *** results show that strategy combining all the control variables is the most cost-effective strategy followed by the strategy involving both personal-protection,the vaccination-only strategy was the least *** vaccination strategy is not cost-effective in this study,it is as effective in curtailing the infection as the other two control *** study suggests that governments of communities with limited resources should consider complementing the use of vaccine with the use of facial mask particularly in hard-to-reach places in their communities.

关键词： neisseria meningitidis Sensitivity analysis Optimal control Cost-effective analysis

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