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Electroacupuncture at the Renzhong (DU 26) and neiguan (pc 6) acupoints repairs the DNA of nervous tissue supplied by the middle cerebral artery in rats

引用

Neural Regeneration Research 2011年第36期6卷 2845-2849页

作者： Xiaorong Chang Mi Liu Jie Yan Ling Fan Wenjin Li Chao Wang Jianqiao Fang The Third Clinical College of Zhejiang Chinese Medical University Hangzhou 310005 Zhejiang Province China Key Subject of Acupuncture Moxibustion and Tuina Key Laboratory of Correlativity between Meridian Point and Viscera Key Laboratory of Biological Information Analysis for Acupuncture and Moxibustion Hunan University of Chinese Medicine Changsha 410007 Hunan Province China

This study compares the changes of DNA repair in brain tissue dominated by the middle cerebral artery after electroacupuncture at the acupoints of Renzhong （DU 26）, neiguan （pc 6）, Quchi （LI 11） and Zusanli （ST 36） in rats. In the apurinic/apyrimidinic endonuclease DNA basic group reparative excision experiments, the apurinic/apyrimidinic endonuclease activity of brain tissue increased slightly after electroacupuncture in rats. In the DNA polymerase β （Pol β）experiments, the Pol β ac-tivity of brain tissue increased after electroacupuncture, especially at DU 26 and pc 6. In the DNA ligase experiments, the expression of DNA ligase 1 in brain tissue increased significantly after electroacupuncture. These findings demonstrate increased activity of apurinic/apyrimidinic en-donuclease, Pol β and DNA ligase 1 after electroacupuncture at DU 26 and pc 6. Also, DNA repair in brain tissue supplied by the middle cerebral artery is promoted after electroacupuncture at DU 26 and pc 6, which are more effective than the LI 11 and ST 36 acupoints .

关键词： electroacupuncture Renzhong （DU 26） neiguan （pc 6） middle cerebral artery DNA repair nervous tissue

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Effects of electroacupuncture on microcirculatory blood flow and glucose transporter function in the hippocampus

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Neural Regeneration Research 2011年第3期6卷 200-205页

作者： Lu, Yan Han, Bingbing Wang, Shijun Shandong Univ Tradit Chinese Med Dept Expt Acupuncturol Acupuncture & Moxibust Coll Jinan 250355 Shandong Peoples R China Shandong Univ Tradit Chinese Med Microcirculat Lab Basic Med Coll Jinan 250355 Shandong Peoples R China

Nerve cell metabolism in post brain ischemia depends on increased microcirculation perfusion and transport function of microvascular endothelial cells. In the present study, a rat model of middle cerebral artery occlusion was established to investigate the influence of electroacupuncture （EA） on hippocampal CA1 cerebral blood flow and glucose transporter 1 （GLUT1） expression in the microvascular endothelial cells. Following EA at neiguan （pc 6）, the cerebral blood flow in the ischemic hippocampal CA1 region was significantly elevated, the number and microvascular integrated absorbance of the GLUTl-positive cells were significantly increased, nerve cell damage was ameliorated, and GLUT1 protein expression in the ischemic hippocampus was significantly increased. Results demonstrate that EA increased the cerebral blood flow of the hippocampal CA1 region and improved the glucose transport function, thereby attenuating neuronal injuries.

关键词： Electroacupuncture neiguan （pc 6） focal cerebral ischemia microvascularendothelial cells glucose transporter 1 cerebral blood flow CA1 region neural regeneration

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