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Implementation of a New Solution for the Preservation of Anatomical Specimens Made of Non-Toxic Substances

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Open Journal of Veterinary Medicine 2024年第3期14卷 56-67页

作者： Ma Reyes Pichardo-Molinero Samantha Jardon-Xicotencatl Misael R. Oliver-González Carlos G. García-Tovar Laboratorio de Apoyo Técnico a la Anatomía Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México Mexico Unidad de Investigación Multidisciplinaria L4 (Morfología Veterinaria y Biología Celular) Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México Mexico

The preservation of anatomical pieces in Veterinary Anatomy is essential since it is not possible to dissect all domestic species. Most techniques use reagents with high levels of toxicity such as formaldehyde. The objective of this work was to develop a new preservation technique that uses reagents with zero toxicity and that allows obtaining preserved pieces suitable for anatomical studies. The alcohol propylene glycol technique was developed, the method of which uses a fixation step with alcohol, sodium chloride, commercial vinegar and subsequently the impregnation of the preservation solution made from propylene glycol and commercial vinegar, which are non-toxic. As a result of this work, adequately preserved sheep hearts were obtained that preserved their morphology with slight changes in size and weight, which did not affect their external and internal anatomical structure. Its coloration was not substantially affected, remaining a little lighter. The pieces obtained showed flexibility which allowed dissections to be carried out. The time to develop the technique was 20 days. A comparative study was carried out with the phenolated glycerin technique that uses toxic reagents (formaldehyde and phenol) and the pieces obtained with the alcohol propylene glycol technique were of better quality, observing that the pieces with phenolated glycerin tend to darken and are more rigid. And the time to develop the technique is 24 days. In conclusion, a preservation technique for anatomical pieces was developed that allowed the preservation of the organs under study, which allow their use for anatomical studies, and which have been preserved without changes until the time of this publication (8 months) and there are pieces preserved with this technique for 2 years.

关键词： Veterinary Anatomy Heart Preservation Techniques Alcohol Propylene Glycol Technique Fixation necropsy Toxic Chemicals

“Alas poor Yorick”: What retrospective analysis of canine skulls can tell us about the impact of environmental factors on health

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Open Journal of Animal Sciences 2013年第3期3卷 12-18页

作者： Luciano O. Valenzuela Kevin Chase Lawrence McGill Shawn Miller Mark Nielsen Karl G. Lark ARUP Animal Reference Pathology Division Salt Lake City USA Department of Biology University of Utah Salt Lake City USA Department of Biology University of Utah Salt Lake City USA Laboratorio de Ecología Evolutiva Humana CONICET Universidad Centro de la Provincia de Buenos Aires Buenos Aires Argentina

Necropsies and extensive histological evaluation for clinical and sub-clinical disease of approximately three hundred Portuguese Water dogs are available as part of an ongoing study to assess their state of health at the end of life. Throughout life these dogs enjoyed a variety of lifestyles and environments. Here we carry out retrospective quantitative assessments of life-time dietary input and physical activity for each dog. To do this, collagens from skull vault bone and from dentine have been analyzed for ratios of stable isotopes to determine differences in diet that individual dogs experienced during late or early life respectively. Robustness of skull bone (weight/unit of skull size) was used as a relative indicator of the amount of physical activity experienced during a dog’s lifetime. These environmental parameters were correlated with the frequency and severity of specific disease processes determined at necropsy. Both measures were shown to exert significant low-level

关键词： Portuguese Water Dog necropsy Histopathology Exercise Nutrition Stable Isotope Skull Dentine Collagen

Prevalence of Canine Heartworm Disease in Stray Dogs of Grenada, West Indies

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Open Journal of Veterinary Medicine 2017年第11期7卷 168-174页

作者： Melissa C. Brown Alfred Chikweto Keshaw P. Tiwari Claude DeAllie Muhammad Iqbal Bhaiyat Ravindra N. Sharma Department of Pathobiology School of Veterinary Medicine St. George’s University Grenada West Indies

Dirofilariasis (heartworm disease) has been reported in Grenadian dogs. The aim of this cross-sectional study was to determine the prevalence of Dirofilaria immitis in stray dogs (n = 523) from the six Parishes of Grenada. necropsy records were reviewed;information on the distribution and lesions of Dirofilaria immitis was recorded. A total of 144 from 523 dogs (27.5%;95% CI: 23.9% to 31.5%) were positive for Dirofilaria immitis on necropsy. This study shows that heartworm disease is prevalent in stray dogs in all the Parishes of mainland Grenada. This is a progressive, life-threatening disease and thus, there is a need to screen and prevent it within the stray dog population of Grenada.

关键词： Dirofilaria immitis Prevalence Stray Dogs necropsy Grenada

Diagnosis and Prevention of Colibacillosis in Chickens

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Animal Husbandry and Feed Science 2019年第5期11卷 149-152页

作者： Shen Xuehuai Zhang Danjun Zhao Ruihong Pan Xiaocheng Yin Lei Hu Xiaomiao Institute of Animal and Veterinary Science Anhui Academy of Agricultural SciencesHefei 230031China Livestock and Poultry Epidemic Disease Research Center of Anhui Province Hefei 230031China

The characteristics of chicken colibacillosis are introduced from the aspects of pathogen,epidemiology,clinical symptoms and necropsy,and clinical comprehensive diagnosis and laboratory diagnosis methods are *** disease is distinguished from diseases with similar symptoms such as chicken chlamydia,chicken streptococcus,chicken necrotic enteritis,broiler ascites syndrome and chicken paratyphoid ***,the preventive and therapeutic measures against the disease are put forward.

关键词： Chicken colibacillosis Pathogen Epidemiology Clinical symptoms necropsy Diagnostic methods Prevention and control measures

Diagnosis and Prevention of Duck Virus Enteritis

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Animal Husbandry and Feed Science 2019年第5期11卷 153-156页

作者： Pan Xiaocheng Zhao Ruihong Shen Xuehuai Yin Lei Zhang Danjun Da Yin Institute of Animal and Veterinary Science Anhui Academy of Agricultural SciencesHefei 230031China Livestock and Poultry Epidemic Disease Research Center of Anhui Province Hefei 230031China

The characteristics of duck virus enteritis(DVE)are introduced from the aspects of pathogen,epidemiology,clinical symptoms,autopsy pathological changes,etc.,and clinical comprehensive diagnosis and laboratory diagnosis methods are *** disease is distinguished from similar diseases including duck necrotic enteritis,duck candidiasis,duck hemorrhage,duck coccidiosis and duck vitamin A ***,the prevention and treatment measures of the disease are put forward.

关键词： Duck virus enteritis Pathogen Epidemiology Clinical symptoms necropsy Diagnostic methods Prevention and control measures