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Absence of human papillomavirus in nasopharyngeal swabs from infants in a population at high risk of human papillomavirus infection

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Pediatric Investigation 2021年第2期5卷 136-139页

作者： Heidi C Smith-Vaughan Allen C Cheng Sepehr N.Tabrizi Danielle F Wurzel Jemima Beissbarth Amanda J Leach Peter S Morris Michael J Binks Paul J Torzillo Anne B Chang Robyn L Marsh Menzies School of Health Research Charles Darwin UniversityDarwinAustralia School of Medicine Griffith UniversityGold CoastAustralia Monash University MelbourneAustralia Murdoch Children's Research Institute The Royal Children’s HospitalMelbourneAustralia Department of Obstetrics and Gynaecology University of MelbourneParkvilleVictoriaAustralia Royal Darwin Hospital DarwinAustralia Royal Prince Alfred Hospital SydneyAustralia Dept of Respiratory and Sleep Medicine I Queensland Children’s HospitalBrisbaneAustralia

Maternal urogenital human papillomavirus(HPV)infection may place neonates at risk of HPV acquisition and subsequently lower respiratory infections as HPV can influence development of *** respiratory HPV prevalence is not known in remote-dwelling Aboriginal infants,who are at high risk of respiratory infection and where the population prevalence of urogenital HPV in women is *** data are necessary to inform HPV vaccination regimens.A retrospective analysis using PCR specific for HPV was performed on 64 stored nasopharyngeal swabs from remote-dwelling Aboriginal infants<6 months of age,with and without hospitalised *** DNA was not detected in any *** the negative result,we cannot exclude a role for HPV in respiratory infections affecting infants in this population;however,our data do not support HPV as an important contributor to acute respiratory infection in remote-dwelling Aboriginal children.

关键词： Human papillomavirus nasopharyngeal swabs Infants

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The sampling efficiencies of commercial nasopharyngeal swabs

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Biosafety and Health 2022年第2期4卷 66-69页

作者： Ying Wang Geng Wang Lulu Zhang Lili Ren Jianwei Wang NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory IPBCAMS-Fondation MérieuxInstitute of Pathogen Biology(IPB)Chinese Academy of Medical Sciences(CAMS)&Peking Union Medical CollegeBeijing 100730China Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Upper respiratory tract samples are the most commonly used samples for coronavirus disease 2019(COVID-19)*** samples collected from the nasopharynx are preferred for viral nucleic acids *** nasopharyngeal swabs(NPSs)are the major factor that influences the sampling *** here evaluated the acceptability and efficiency of NPSs from five manufacturers by examining the concentration of glyceraldehyde-3-phosphate dehydrogenase gene(GAPDH)retrieved from the swabs using the RT-PCR *** different concentrations of GAPDH were detected,ranged from 4.36×10^(8) copies/mL to 6.98×10^(10) copies/mL among the five swabs(P<0.05).The designation of the swab head,with or without tip expansion,had limited influence on the collection *** discrepancy among the NPSs emphasized the improvement of the swab head material.

关键词： nasopharyngeal swabs Nucleic acids Efficiency Designation

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Safety management of nasopharyngeal specimen collection from suspected cases of coronavirus disease 2019

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International Journal of Nursing Sciences 2020年第2期7卷 153-156页

作者： Yan Qian Tieying Zeng Hui Wang Min Xu Junhua Chen Na Hu Daiqi Chen Yu Liu Tongji Hospital Tongji Medical College of Huazhong University of Science&TechnologyWuhanHubeiChina

This article introduces safety management strategies of nasopharyngeal specimen collection from suspected cases of coronavirus disease 2019 in a tertiary designated *** key points include establishing a special sampling room,strict sterilization of the entire environment,training of professional nurses,enhancement of personal protection,standardization of methods and processes for swab collection,and a timely and safety sample *** than 11,000 nasopharyngeal specimens were collected by eight nurses,with an average of 1,375 specimen swab collections each nurse,and no one was infected.

关键词： Coronavirus disease 2019 nasopharyngeal swabs Safety management Specimen collection

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