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Niosome-encapsulated auraptene reduced the mRNA expression of VEGF-A and PDGFs genes in human retinaderived RPE cell line

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1028-1035页

作者： Akram Vahidi Teymoor Khosravi Farzad Dastaviz Mehdi Sheikh Arabi Ayyoob Khosravi Morteza Oladnabi Student Research Committee Golestan University of Medical SciencesGorgan ***Iran Department of Medical Nanotechnology Faculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran Stem Cell Research Center Golestan University of Medical SciencesGorgan ***Iran Department of Molecular Medicine Faculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran Gorgan Congenital Malformations Research Center Golestan University of Medical SciencesGorgan ***Iran

AIM:To evaluate the effect of auraptene(AUR)treatment in forms of free and encapsulated in niosome nanoparticles by investigating the mRNA expression level of vascular endothelium growth factor(VEGF)-A and platelet-derived growth factors(PDGFs)in human retinal pigment epithelium(RPE)cell line.METHODS:Niosome nanocarriers were produced using two surfactants Span 60 and Tween 80.RPE cell line was treated with both free AUR and niosome-encapsulated.Optimum dosage of treatments was calculated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Expression of VEGF-A and PDGF-A,PDGF-B,PDGF-C,PDGF-D genes was measured after total RNA extraction and cDNA synthesis,using real-time polymerase chain reaction(RT-PCR).RESULTS:The highest entrapment efficiency(EE)was achieved by Span 60:cholesterol(1:1)with 64.3%.The half maximal inhibitory concentration(IC50)of free and niosome-encapsulated AUR were 38.5 and 27.78µg/mL,respectively.Release study revealed that niosomal AUR had more gradual delivery to the cells.RT-PCR results showed reduced expression levels of VEGF-A,PDGF-A,PDGF-B,PDGF-C,and PDGF-D after treatment with both free and niosomal AUR.CONCLUSION:Niosomal formulation of Span 60:cholesterol(1:1)is an effective drug delivery approach to transfer AUR to RPE cells.VEGF-A,PDGF-A,PDGF-B,PDGF-C,and PDGF-D are four angiogenic factors,inhibiting which by niosomal AUR may be effective in age-related macular degeneration.

关键词： auraptene niosome age-related macular degeneration angiogenesis nanocarrier

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Drug delivery with Mn-doped MoO2 for photothermalenhanced chemotherapy in fighting cancers

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Rare Metals 2024年第5期43卷 2230-2240页

作者： Ping Zhao Hai-Fa Zheng Ju Peng Xing-Long Li Fazal Raziq Xiao-Jun Liu Hao-Quan Yu Jin-Lu Tang Yi-Fan Kang Wen-Huan Huang School of Chemistry and Chemical Engineering Guangdong Pharmaceutical University KAUST Catalysis Center King Abdullah University of Science and Technology 23955-6900 Thuwal Key Laboratory of Chemical Additives for China National Light Industry College of Chemistry and Chemical EngineeringShaanxi University of Science and Technology

Although chemotherapy has been intensively applied in cancer treatments,its inadequate therapeutic efficacy and severe side effects are still under constant concerns.Nanoplatforms used as anti-tumor drug delivery system （DDS） have attracted tremendous attentions owing to their various intriguing properties.Herein,Mn-doped Mo O2nanoparticles coated with Zr O2and capped with Bi2O3have been designed as a DDS,namely MMZB.MMZB possesses good magnetic properties,great photothermal conversion ability,sensitive tumor microenvironment （TME） responsiveness,and good biocompatibility in hemocompatibility in vitro.Thus,MMZB has been utilized to load the chemotherapeutic agent daunomycin（DNM）(MMZB@DNM) for chemo-photothermal combined therapy.MMZB@DNM demonstrates a more impressive anti-cancer effect than the individual photothermal or chemotherapy both in vitro and in vivo.Furthermore,the analysis of tumor specimen sections and serum levels after the treatment indicates negligible side effects for MMZB@DNM in vivo.This contribution provides a valuable concept in designing therapeutic agents for achieving significantly enhanced tumor treatments,which benefits from the synergistic combination of chemotherapy and photothermal therapy in one single nanoagent.

关键词： Mn-doped MoO 2 Bi 2 O 3 nanocarrier Photothermal Chemotherapy

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nanocarrier-delivered dsRNA suppresses wing development of green peach aphids

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Insect Science 2022年第3期29卷 669-682页

作者： Yun-Hui Zhang Zhong-Zheng Ma Hang Zhou Zi-Jian Chao Shuo Yan Jie Shen Department of Plant Biosecurity and MOA Key Lab of Pest Monitoring and Green Management College of Plant ProtectionChina Agricultural UniversityBeijingChina

RNA interference(RNAi)has developed rapidly as a potential“green”pest management strategy.At present,most studies have focused on the screening of aphid lethal genes,whereas only a few studies have been conducted on wing development,which is crucial for aphid migration and plant-virus dissemination.Here,the Myzus persicae genes vestigial(vg)and Ultrabithorax(Ubx)related to wing development,were cloned.These two genes were expressed in various tissues of 3rd-instar winged aphids.The mRNA level of vg was high in 3rd-instar nymphs,whereas the expression level of Ubx was high in adults.The nanocarrier-mediated delivery system delivered double-stranded RNAs for aphid RNAi using topical and root applications.The expression levels of vg and Ubx were downregulated by 44.0%and 36.5%,respectively,using the topical application.The simultaneous RNAi of the two target genes caused 63.3%and 32.2%wing aberration rates using topical and root applications,respectively.The current study provided a promising method for controlling aphid migration to alleviate the spread of insect transmitted plant diseases.

关键词： nanocarrier RNA interference Ultrabithorax vestigial wing development

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抗原改造联合纳米肿瘤疫苗用以克服肿瘤新抗原短缺及在多种肿瘤类型上激起强效抗肿瘤免疫反应

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Science Bulletin 2024年第7期69卷 922-932页

作者：陈宏宇黄子超李佳玄董思徐玉笛马胜赵佳雨刘丽萍孙天盟宋万通陈学思 School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China Key laboratory of Polymer Ecomaterials Changchun Institue of Applied ChemistryChinese Academy of SciencesChangchun 130022China Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education Institute of ImmunologyThe First HospitalJilin UniversityChangchun 130021China Jilin Biomedical Polymers Engineering Laboratory Changchun 130022China

Neoantigen cancer vaccines have been envisioned as one of the most promising means for cancer therapies.However,identifying neoantigens for tumor types with low tumor mutation burdens continues to limit the effectiveness of neoantigen vaccines.Herein,we proposed a "hit-and-run" vaccine strategy which primes T cells to attack tumor cells decorated with exogenous "neo-antigens".This vaccine strategy utilizes a peptide nanovaccine to elicit antigen-specific T cell responses after tumor-specific decoration with a nanocarrier containing the same peptide antigens.We demonstrated that a poly(2-oxazoline)s(POx) conjugated with OVA_(257-264) peptide through a matrix metalloprotease 2(MMP-2) sensitive linker could efficiently and selectively decorate tumor cells with OVA peptides in vivo.Then,a POx-based nanovaccine containing OVA_(257-264) peptides to elicit OVA-specific T cell responses was designed.In combination with this hit-and-run vaccine system,an effective vaccine therapy was demonstrated across tumor types even without OVA antigen expression.This approach provides a promising and uniform vaccine strategy against tumors with a low tumor mutation burden.

关键词： Cancer immunotherapy Cancer vaccine Neoantigen nanocarrier Poly(2-oxazoline)s

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Novel nanocarrier for promoting tumor synergistic therapy by down-regulation of heat shock proteins and increased Fe^(3+) supply

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Science China Chemistry 2023年第4期66卷 1078-1085页

作者： Yu Lu Peng Zhang Yifei Zhou Ruohao Zhang Xinyu Fu Jing Feng Hongjie Zhang Department of Chemistry Jilin UniversityChangchun 130012China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130012China Department of Radiology The Second Hospital of Jilin UniversityChangchun 130041China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China Department of Chemistry Tsinghua UniversityBeijing 100084China

With the emergence of new therapeutic methods,synergistic therapy has attracted great attention because it can improve the treatment efficacy,and reduce the toxic side effects.Herein,we developed a nanocarrier BGT by co-loading glucose oxidase(GOD)and transferrin(TRF)on the porous Bi nanoparticles(NPs)for improving tumor synergistic therapy.GOD endows BGT with catalytic capacity of decomposing glucose into gluconic acid and a large amount of H2O2 for starving therapy.H2O2 further destroys TRF structure and releases Fe^(3+),which could react with H2O2 to generate highly toxic·OH for chemodynamic therapy(CDT).In addition,GOD-induced glucose depletion and decreased expression of heat shock proteins(HSPs)can also alleviate the thermotolerance of tumor cells to improve the efficiency of mild photothermal therapy(PTT).Mild temperature can in turn promote the production of reactive oxygen species(ROS)for improving the synergistic therapy.Combined with the excellent targeting ability of TRF,efficient tumor synergistic therapy can be achieved.This work shows that BGT has good photothermal stability and biocompatibility,and can be used as a nanocarrier,providing an effective method for collaborative therapy of tumor.

关键词： nanocarrier mild temperature photothermal therapy starvation therapy chemodynamic therapy

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nanocarrier-mediated co-delivery of chemotherapeutic drugs and gene agents for cancer treatment

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Acta Pharmaceutica Sinica B 2015年第3期5卷 169-175页

作者： Lin Kang Zhonggao Gao Wei Huang Mingji Jin Qiming Wang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing City Key Laboratory of Drug Delivery Technology and Novel FormulationsInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College

The efficacy of chemotherapeutic drug in cancer treatment is often hampered by drug resistance of tumor cells,which is usually caused by abnormal gene expression.RNA interference mediated by si RNA and mi RNA can selectively knock down the carcinogenic genes by targeting specific m RNAs.Therefore,combining chemotherapeutic drugs with gene agents could be a promising strategy for cancer therapy.Due to poor stability and solubility associated with gene agents and drugs,suitable protective carriers are needed and have been widely researched for the co-delivery.In this review,we summarize the most commonly used nanocarriers for co-delivery of chemotherapeutic drugs and gene agents,as well as the advances in co-delivery systems.

关键词： nanocarrier Co-delivery Chemotherapeutic drug Gene Liposome Micelle Dendrimer Supramolecular system

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Gene transfection mediated by polyethyleneiminepolyethylene glycol nanocarrier prevents cisplatininduced spiral ganglion cell damage

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Neural Regeneration Research 2015年第3期10卷 425-431页

作者： Guan-gui Chen Min Mao Li-zi Qiu Qi-ming Liu Department of Otorhinolaryngology the Second Affiliated Hospital of Guangzhou Medical University

Polyethyleneimine-polyethylene glycol （PEI-PEG）, a novel nanocarrier, has been used for trans- fection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene transfer in spiral ganglion cells. It remains unclear whether PEI-PEG could be used for gene therapy with X-linked inhibitor of apoptosis protein （XIAP） in the inner ear. In the present study, we performed PEI-PEG-mediated XIAP gene transfection in the cochlea of Sprague-Dawley rats, via scala tympani fenestration, before daily cisplatin injections. Audito- ry brainstem reflex tests demonstrated the protective effects of XIAP gene therapy on auditory function. Immunohistochemical staining revealed XIAP protein expression in the cytoplasm of cells in the spiral ganglion, the organ of Corti and the stria vascularis. Reverse transcription-PCR detected high levels of XIAP mRNA expression in the cochlea. The present findings suggest that PEI-PEG nanocarrier-mediated XIAP gene transfection results in XIAP expression in the cochlea, prevents damage to cochlear spiral ganglion cells, and protects hearing.

关键词： nerve regeneration polyethyleneimine-polyethylene glycol spiral ganglion cells X-linkedinhibitor of apoptosis protein gene therapy nanocarrier cisplatin neural regeneration ototoxicity cochlea

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Graphene oxide-based hydrogels as a nanocarrier for anticancer drug delivery

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Nano Research 2019年第5期12卷 973-990页

作者： Abdullah A.Ghawanmeh Gomaa A.M.Ali H.Algarni Shaheen M.Sarkar Kwok Feng Chong Faculty of Industrial Sciences & Technology Universiti Malaysia PahangGambang26300 KuantanMalaysia Chemistry Department Faculty of ScienceAl-Azhar UniversityAssiut71524Egypt Department of Physics Faculty of SciencesKing Khalid UniversityAbha 61413P. O. Box 9004Saudi Arabia Research Center for Advanced Materials Science (RCAMS) King Khalid UniversityAbha 61413P. O. Box 9004Saudi Arabia Bernal Institute Department of Chemical SciencesUniversity of LimerickCastletroyV94 LimerickIreland

Graphene oxide (GO) possesses excellent mechanical strength,biocompatibility,colloidal stability,large surface area and high adsorption capability.It has driven to cancer nanotechnology to defeat cancer therapy obstacles,via integration into three-dimensional (3D) hydrogel network with biocompatible polymers as nanocomposites carrier,and controllable release of anticancer drugs.Specifically,the surface of GO affords π-π stacking and hydrophilic interactions with anticancer drugs.Additionally,modification of GO with various polymers such as natural and synthetic polymers enhances its biodegradability,drug loading,and target delivery.In this review,GO based hydrogels research accomplishments are reviewed on the aspects of crosslinking strategies,preparation methods,the model drug,polymer conjugation and modification with targeting ligands.Moreover,swelling kinetics,drug release profile and biological activity in vivo and in vitro are discussed.The biocompatibility of GO based hydrogels is also discussed from the perspective of its nano-bio interfaces.Apart from that,the clinical potential of GO based hydrogels and its major challenges are addressed in detail.Finally,this review concludes with a summary and invigorating future perspectives of GO based hydrogels for anticancer drug delivery.It is anticipated that this review can stimulate a new research gateway to facilitate the development of anticancer drug delivery by harnessing the unique properties of GO based hydrogels,such as large surface area,chemical purity,high loading capacity of drug,chemical stability,and the nature of lipophilic for cell membrane penetration.

关键词： graphe ne oxide cancer nan otechnology hydrogel nanocarrier drug delivery

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In vitro Anticancer Efficacy by Magnetic Targeted nanocarrier with Local Delivery of Paclitaxel

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Chemical Research in Chinese Universities 2016年第1期32卷 149-154页

作者： ZHANG Shubin QIAN Xue ZHANG Daihui ZHU Jinming WU Yi GUO Yi XU Li Key Laboratory for Molecular Enzymology and Engineering Ministry of Education School of Life Sciences Jilin University Changehun 130012 P. R. China College of Pharmacy Jilin University Changchun 130021 P. R. China Jilin Entry-exit Inspection and Quarantine Bureau Changchun 130062 P. R. China China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China

A magnetic nanoparticles-loaded polymeric nanocarrier was developed. Amphiphilic copolymer, methoxy polyethylene glycol-poly（D,L-lactide-co-glycolide）（MPEG-PLGA） could self-assemble to form nanomicelle with the help of emulsion-solvent evaporation technique. This nanocarrier with core-shell structure was loaded with magnetic iron oxide nanoparticles（IONPs） and anticancer drug paclitaxel（PTX）. The hydrodynamic diameter of IONPs-PTX-loaded nanocarrier showed an average size of 110 nm with a polydispersity index（PDI） of 0.136, and its zeta potential was （-4.76±0.36） mV. The drug-loading content and encapsulation efficiency were 4.47% and 31.28%, respectively. In vitro drug release experiment was performed and a sustained release profile was observed. 3-（4,5-Dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（MTT） assay indicated that IONPs-PTX-loaded nanocarrier showed comparable cytotoxicity with free paclitaxel. When an external magnetic field was applied, the nanocarrier significantly localized at the target area, demonstrating that the nanocarrier could be used for potential magnetic targeted drug delivery.

关键词： Magnetic nanoparticle Paclitaxel nanocarrier Local delivery

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Effects of the Molecular Weight of PLGA on Degradation and Drug Release In vitro from an mPEG-PLGA nanocarrier

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Chemical Research in Chinese Universities 2016年第5期32卷 848-853页

作者： LIU Rui WANG Yan MA Yudan WU Yi GUO Yi XU Li Key laboratory for Molecular Enzymology and Engineering Ministry of Education National Engineering Laboratory for AIDS Vaccine School of Life Sciences Jilin University Changchun 130012 P. R. China Department of Endocrinology China-Japan Union Hospital Jilin University Changehun 130033 P. R. China College of Pharmacy Jilin University Changchun 130021 P. R. China Jilin Research Institute of Sports Science Changchun 130022 P. R. China

We successfully synthesized four kinds of copolymers with varying molecular weights of poly（lactide- co-glycolide）（PLGA） to yield methoxy-poly（ethylene glycol）-block-poly（lactide-co-glycolide）（mPEG-PLGA） nano- carriers： mPEG-PLGA（3k）, mPEG-PLGA（9k）, mPEG-PLGA（llk） and mPEG-PLGA（16k）. An antitumor drug, 10-hydroxycamptothecin（HCPT）, was encapsulated into the mPEG-PLGA nanocarrier cores by self-assembly in dialysis. The lower molecular weight nanocarriers degraded more quickly, resulting in mass loss, pH decline, and a rapid HCPT release rate in vitro. The degradation and drug release of the nanocarriers were dependent on the PLGA molecular weight. However, the larger molecular weight nanocarriers could not increase the loading content and encapsulation efficiency. Considering the antitumor effect of these nanocarriers, the mPEG-PLGA（9k） nanocarrier, which had the highest drug loading content[（7.72±0.57）%] and a relatively high encapsulation efficiency [（22.71±5.53）%], is an optimum agent for drug delivery.

关键词： Poly（lactide-co-glycolide） 10-Hydroxycamptothecin nanocarrier Drug delivery

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