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Mitochondrial Autophagy and nlrp3 Inflammasome in Pulmonary Tissues from Severe Combined Immunodeficient Mice after Cardiac Arrest and Cardiopulmonary Resuscitation

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Chinese Medical Journal 2018年第10期131卷 1174-1184页

作者： Jing-Jun Lyu Jawahar L Mehta Yi Li Lu Ye Sheng-Nan Sun Hong-Shuang Sun Jia-Chang Li Dong-Mei Zhang Jie Wei Department of Emergency Renmin Hospital o1 Wuhan University Wuhan Hubei 430060 China Department of Medicine Central Arkansas Veterans Healthcare System University of Arkansas for Medical Sciences Little Rock AR 72205 USA Department of Emergency Peking Union Medical College Hospital Beijing 100032 China

Background：The incidence of cancer,diabetes,and autoimmune diseases has been ***,there are more and more patients with solid organ *** survival rate of these immunocompromised individuals is extremely low when they are severely *** this study,we established cardiac arrest cardiopulmonary resuscitation （CPR） model in severe combined immunodeficient （SCID） mice,analyzed the expression and activation of mitochondrial autophagy and nlrp3 inflammasome/caspase-1,and explored mitochondrial repair and inflammatory injury in immunodeficiency individual during systemic ischemia-reperfusion ***：A potassium chloride-induced cardiac arrest model was established in C57BL/6 and nonobese diabetic/SCID （NOD/SCID） *** hundred male C57BL/6 mice and 100 male NOD/SCID mice were randomly divided into five groups （control,2 h post-CPR,12 h post-CPR,24 h post-CPR,and 48 h post-CPR）.A temporal dynamic view of alveolar epithelial cells,macrophages,and neutrophils from bronchoalveolar lavage fluid （BALF） was obtained using Giemsa *** characterization ofphenotypic analysis of macrophages in the lung interstitial tissue was analyzed by flow *** morphological changes ofmitochondria 48 h after CPR were studied by transmission electron microscopy and quantified according to the Flameng grading *** blotting analysis was used to detect the expression and activation of the markers of mitochondrial autophagy,nlrp3 inflammasome,and ***：（1） In NOD/SCID mice,macrophages were disintegrated in BALF,and many alveolar epithelial cells were shed at 48 h after *** with C57BL/6 mice,the ratio of macrophages/total cells peaked at 12 h and was significantly higher in NOD/SCID mice （31.17 ± 4.13 vs.49.69 ± 2.43,t =14.46,P =0.001）.After 24 h,the results showed a downward ***,a large number of macrophages were disintegrated in the BALF.（2） Mitochondrial autophagy was present in both C

关键词： Autophagy Cardiopulmonary Resuscitation Immunodeficiency Macrophage Mitochondria nlrp3 inftammasome

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