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Journal of Environmental Sciences 2014年第12期26卷 2526-2534页

作者： Xiuli Dong Eric McCoy Mei Zhang Liju Yang Department of Pharmaceutical Sciences Biomanufacturing Research Institute and Technology Enterprise (BRITE) North Carolina Central UniversityDurham NC USA Department of Industrial & Manufacturing Engineering Florida State University Tallahassee FL USA

Multi-walled carbon nanotube（MWCNT） sheet was fabricated from a drawable MWCNT forest and then deposited on poly（methyl methacrylate） film. The film was further coated with a natural antimicrobial peptide nisin. We studied the effects of nisin coating on the attachment of Bacillus anthracis spores, the germination of attached spores, and the subsequent biofilm formation from attached spores. It was found that the strong adsorptivity and the super hydrophobicity of MWCNTs provided an ideal platform for nisin coating. nisin coating on MWCNT sheets decreased surface hydrophobicity, reduced spore attachment, and reduced the germination of attached spores by 3.5 fold, and further inhibited the subsequent biofilm formation by 94.6% compared to that on uncoated MWCNT sheet. nisin also changed the morphology of vegetative cells in the formed *** results of this study demonstrated that the anti-adhesion and antimicrobial effect of nisin in combination with the physical properties of carbon nanotubes had the potential in producing effective anti-biofilm formation surfaces.

关键词： Multi-walled carbon nanotubes (MWCNTs) Biofilm Bacillus anthracis nisin

Fabrication and characterization of gallic-acid/nisin loaded electrospun core/shell chitosan/polyethylene oxide nanofiberous membranes with free radical scavenging capacity and antimicrobial activity for food packing applications

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Food Bioscience 2023年第3期53卷 715-723页

作者： Farzaneh Tajfiroozeh Ali Moradi Fakhri Shahidi Jebrail Movaffagh Hossein Kamali Sahar Roshanak Azadeh Shahroodi Dept.of Food Science and Technology Faculty of AgricultureFerdowsi University of MashhadMashhadIran Orthopedic Research Center Mashhad University of Medical SciencesMashhadIran Clinical Research Development Unit Ghaem HospitalMashhad University of Medical SciencesMashhadIran Targeted Drug Delivery Research Center Pharmaceutical Technology InstituteMashhad University of Medical ScienceMashhadIran Dept.of Pharmaceutical Nanotechnology School of PharmacyMashhad University of Medical ScienceMashhadIran

Food-friendly packaging are supposed to keep the food quality and freshness in terms of moisture and odour and protect foods against microbial putrefying contaminants and oxidant *** study has aimed to fabricate biodegradable electrospun chitosan/polyethylene oxide membranes loaded with gallic acid and nisin for antioxidant/antibacterial *** acid/nisin loaded core-shell chitosan/polyethylene oxide nanofiberous membranes were fabricated through electrospinning and characterized by transmission and scanning electron microscopy,fourier-transform infrared spectroscopy,and differential scanning calorimetry as well as antioxidant and antibacterial *** acid(10%)/nisin(0.05%and 0.07%)loaded membranes showed maximal tensile strength as well as antioxidant and antimicrobial effects for 20 and 60 days,*** electrospun gallic acid/nisin loaded chitosan/polyethylene oxide membranes possess antioxidant and antibacterial properties and can be suggested as potential food packaging membranes.

关键词： Chitosan Gallic acid nisin Electrospinning Food packaging Shelf life

Effects of chitosan oligosaccharide-nisin conjugates formed by Maillard reaction on the intestinal microbiota of high-fat diet-induced obesity mice model

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Food Quality and Safety 2019年第3期3卷 169-177页

作者： Xiaojie Zheng Xin Zhang Chunhua Xiong School of Food Science and Biotechnology Zhejiang Gongshang UniversityHangzhou Department of Food Science and Engineering School of Marine SciencesNingbo UniversityP.R.China

Objectives:The goal of this study was to evaluate the modulatory effect of chitosan oligosaccharide-nisin conjugate(CON-C)on intestinal microbiota of human flora-associated(HFA)mice and also reveal its effect towards the high-fat diet(HFD)-induced *** Chitosan oligosaccharides and nisin showed great potential in modulating the intestinal microbiota,so it is worth to explore whether the modulation effect of chitosan oligosaccharide could be improved by covalently binding with *** and Methods:CON-C was prepared by heating the mixed solution of chitosan oligosaccharide and nisin at 80℃ and pH 2.0 for *** structure of CON-C were analyzed by Fourier transform-infrared spectroscopy(FT-IR)and X-ray diffraction(XRD).The CON-C’s anti-obesity effect and modulatory effect toward intestinal microbiota were analyzed using human flora-associated(HFA)mice ***:CON-C could alleviated HFD-induced gut dysbiosis,by significantly decreasing the numbers of Bifidobacterium and Lactobacillus/Enterococcus spp.,and increasing the numbers of Bacteroides-Prevotella and Clostridium ***-C could also enriched the most differentially expressed genes through KEGG pathways of biosynthesis of amino acids,two-component system,and ATP binding cassette(ABC)***:The improved therapeutic effect of CON-C against HFD-induced obesity has been approved,and hence,CON-C has a great potential to be utilized as a functional food ingredient in reducing body weight.

关键词： chitosan oligosaccharide nisin Maillard reaction modulatory effect intestinal microbiota

A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第8期8卷 549-554页

作者： WANG Fang CAO Li-ting HU Song-hua Department of Veterinary Medicine College of Animal Sciences Zhejiang University Hangzhou 310029 China

The objective of this study is to propose a more accurate and faster MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r2=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125～32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7～8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24～28 h for ADA. The MCA provides an accurate and rapid method for quantifi-cation of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.

关键词： MTT colorimetric assay nisin Micrococcus luteus

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1995年第4期15卷 193-197页

作者：叶嗣颖 Department of Microbiology Tongji Medical University Wuhan Institute of Biotechnology University of Helsinki Finland

In this study, a plasmid was integrated into nisP, creating the first defined mutation in a nisin biosynthetic gene. The mutant strain secreted fully modified nisin with the N-terminal leader still *** presence of the leader was confirmed by N-terminal sequencing of the purified precursor. The dehydration and lanthionine formation of the precursor were already completed as active nisin could be formed by cleaving the leader from the inactive precursor by a trypsin treatment or by incubation with wild type cells. nisin immunity of the NisP mutant strain was lowered to about 10% of the wild type immunity. The results show that NisP is needed for precursor processing and for development of high immunity of nisin.

关键词： nisin Lactococcus lactis nisin immunity gene mutation

nisin对奶牛乳腺炎葡萄球菌生物被膜形成的影响及调节机制

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中国食品学报 2017年第10期17卷 8-15页

作者：许女史改玲张浩杜立红王俊东食品科学与工程学院山西农业大学山西太谷030801 山西省生态畜牧与环境兽医学重点实验室山西农业大学山西太谷030801

本文考察了nisinA对奶牛乳腺炎葡萄球菌生物被膜的形成及相关基因表达的影响。结果表明,亚抑菌浓度的nisinA对金黄色葡萄球菌SMS10和表皮葡萄球菌SMSE169的生长和生物被膜的形成都起到明显的抑制作用;当nisinA的浓度为8×MIC和16MIC时,... 详细信息

本文考察了nisinA对奶牛乳腺炎葡萄球菌生物被膜的形成及相关基因表达的影响。结果表明,亚抑菌浓度的nisinA对金黄色葡萄球菌SMS10和表皮葡萄球菌SMSE169的生长和生物被膜的形成都起到明显的抑制作用;当nisinA的浓度为8×MIC和16MIC时,其对菌株已形成的成熟生物被膜也具有良好的消除作用;采用电镜可以观察到经nisinA处理的生物被膜的密度和厚度显著降低,不仅出现了明显空隙,而且可见单个分散的菌体细胞。实时荧光定量PCR结果表明,相比于对照组,nisinA处理组的ica D,agr基因的表达显著提高,clfB基因的表达却显著下降,而关于RNAIII基因的表达情况,2株菌呈现出不同的变化趋势,显示出生物被膜基因调控的复杂性。nisinA天然、安全、高效等特点,将在奶牛乳房炎的预防和治疗上具有良好的开发前景。

关键词： nisin 奶牛乳腺炎葡萄球菌生物被膜

nisin、ε-聚赖氨酸、pH值对虾源枯草芽孢杆菌生长/非生长界面模型构建与评价

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食品科学 2020年第22期41卷 140-147页

作者：郭全友刘玲李保国杨絮姜朝军中国水产科学研究院东海水产研究所上海200090 上海理工大学医疗器械与食品学院上海200093

以分离自熟制南美白对虾的虾源枯草芽孢杆菌为对象,探究乳酸链球菌肽(nisin)、ε-聚赖氨酸(ε-polylysine,ε-PL)和pH值对枯草芽孢杆菌的抑制效应,采用简单Logistic及多项式Logistic方程构建其生长/非生长界面模型,并对模型进行验证,比... 详细信息

以分离自熟制南美白对虾的虾源枯草芽孢杆菌为对象,探究乳酸链球菌肽(nisin)、ε-聚赖氨酸(ε-polylysine,ε-PL)和pH值对枯草芽孢杆菌的抑制效应,采用简单Logistic及多项式Logistic方程构建其生长/非生长界面模型,并对模型进行验证,比较分析抑菌因子间的协同或拮抗效应。结果表明,建立简单Logistic模型与多项式Logistic模型的R^2-Nagelkerke值分别为0.79和0.91;Hosmer-Lemeshow测试分别为χ^2=2.00、P=0.981(简单Logistic模型)和χ^2=0.76、P=1(多项式Logistic模型),说明两种模型均能很好描述nisin、ε-PL、pH值及其交互作用下枯草芽孢杆菌生长/非生长情况(Pnisin、ε-PL、pH值及其交互作用对虾源枯草芽孢杆菌生长/非生长界面影响显著(Pnisin及ε-PL质量浓度增加或pH值降低,生长区域逐渐减小,非生长区域逐渐扩大,枯草芽孢杆菌的生长受到抑制,当nisin质量浓度增加,枯草芽孢杆菌的生长/非生长界限向更高ε-PL质量浓度、更低pH值方向移动。无论第3种因子质量浓度为何值,当nisin质量浓度为60、100、140、180μg/mL时,枯草芽孢杆菌在pH值分别为4.0、4.25、4.5、5.0时表现为不生长。ε-PL质量浓度为60、100、140、180μg/mL时,枯草芽孢杆菌pH值为4.0、4.5、4.75、5.25时生长概率均为0。当pH<5.0时,枯草芽孢杆菌的生长受到较强抑制,此时pH值是影响枯草芽孢杆菌生长/非生长界限的主要因素。通过构建枯草芽孢杆菌生长/非生长概率模型,为定量描述水产品抑菌效应和风险评价提供一种有效手段。

关键词：枯草芽孢杆菌生长/非生长模型 nisin ε-聚赖氨酸抑菌效应 pH值

nisin对酸肉常温贮藏中营养卫生及感官品质的影响

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食品与发酵工业 2019年第23期45卷 176-182页

作者：罗珍岑段珍珍谭小琴周才琼西南大学食品科学学院重庆400715 重庆市特色食品工程技术研究中心重庆400715

采用不同nisin浓度处理酸肉产品并真空包装,研究酸肉常温保藏中营养、卫生及感官品质的变化以确定酸肉最佳保藏时间。结果表明,酸肉中蛋白质和脂肪含量在贮藏中逐渐下降,其降解产物非蛋白氮(nonprotein nitrogen,NPN)、挥发性盐基氮(tot... 详细信息

采用不同nisin浓度处理酸肉产品并真空包装,研究酸肉常温保藏中营养、卫生及感官品质的变化以确定酸肉最佳保藏时间。结果表明,酸肉中蛋白质和脂肪含量在贮藏中逐渐下降,其降解产物非蛋白氮(nonprotein nitrogen,NPN)、挥发性盐基氮(total volatile basic nitrogen,TVB-N)、游离脂肪酸和硫代巴比妥酸(thiobarbituric acid,TBA)随nisin添加量增加呈下降趋势,且均低于对照组;在保藏80 d内,nisin各剂量组菌落总数均未超标。以肉腐臭的TBA阈值评判,对照组贮藏时间不宜超过26 d,nisin添加中、低、高剂量组分别不宜超过30、37和42 d。以国家腌肉TVB-N一级品限量看,对照组贮藏时间不宜超过30 d,nisin各组不宜超过45 d。通过分析蛋白质和脂肪降解产物、菌落总数及感官评定,酸肉采用真空包装后常温贮藏时间不宜超过20 d,nisin低、中剂量组不宜超过30 d,高剂量组不宜超过45 d。该研究可为酸肉产品商业化及延伸地方特色食品产业链提供参考。

关键词：酸肉 nisin 常温保藏营养卫生感官品质

nisin、ε-聚赖氨酸和温度对枯草芽孢杆菌失活动力学的影响

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食品与发酵工业 2019年第11期45卷 58-64,70页

作者：郭全友刘玲李保国姜朝军中国水产科学研究院东海水产研究所上海200090 上海理工大学医疗器械与食品学院上海200093

以源自熟制对虾虾仁的优势腐败菌枯草芽孢杆菌为对象,对温度(25、37和45℃)、nisin和ε-聚赖氨酸在酸性环境(pH=5)作用下其失活效应进行分析,并采用线性模型、Weibull模型、Log-logistic模型对不同处理条件下枯草芽孢杆菌的失活动力学... 详细信息

以源自熟制对虾虾仁的优势腐败菌枯草芽孢杆菌为对象,对温度(25、37和45℃)、nisin和ε-聚赖氨酸在酸性环境(pH=5)作用下其失活效应进行分析,并采用线性模型、Weibull模型、Log-logistic模型对不同处理条件下枯草芽孢杆菌的失活动力学进行拟合和评价。结果表明:25~45℃时,枯草芽孢杆菌失活量随nisin和ε-聚赖氨酸的浓度变化而变化。温度由25℃升高至45℃,nisin和ε-聚赖氨酸作用下枯草芽孢杆菌失活量呈先升后降趋势,37℃时失活效果最佳,25℃和45℃时,ε-聚赖氨酸作用下的失活效果优于nisin,37℃时,两者差异不显著。处理180min终点枯草芽孢杆菌数均降至0。Log-logistic模型与Weibull模型均能很好拟合其失活过程,而线性模型难以描述失活动力学,Log-logistic模型拟合优度优于Weibull模型。通过构建虾源枯草芽孢杆菌在温度、nisin和ε-聚赖氨酸作用下失活模型,为优化靶向生物杀菌和延长水产品货架期等提供依据。

关键词：枯草芽孢杆菌 nisin ε-聚赖氨酸温度失活

含肉桂油和nisin的海藻酸钠薄膜保鲜黑鱼性能分析

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农业机械学报 2011年第5期42卷 146-150页

作者：吕飞丁玉庭叶兴乾浙江工业大学生物工程与环境学院杭州310014 浙江大学生物系统工程与食品科学学院杭州310029

研究了含有肉桂油和nisin的海藻酸钠抗菌薄膜保鲜(4±1)°C下贮藏黑鱼鱼肉的效果。鱼肉片被分为:未处理组(CK),海藻酸钠薄膜组(C0),肉桂油海藻酸钠薄膜组(C1),nisin海藻酸钠薄膜组(C2),肉桂油和nisin海藻酸钠薄膜组(C3)。相对于CK、C0和... 详细信息

研究了含有肉桂油和nisin的海藻酸钠抗菌薄膜保鲜(4±1)°C下贮藏黑鱼鱼肉的效果。鱼肉片被分为:未处理组(CK),海藻酸钠薄膜组(C0),肉桂油海藻酸钠薄膜组(C1),nisin海藻酸钠薄膜组(C2),肉桂油和nisin海藻酸钠薄膜组(C3)。相对于CK、C0和C2,C1和C3能更好抑制鱼肉总嗜温菌、总嗜冷菌和假单胞菌。C1和C3可显著抑制鱼肉的腐败变质,维持较低pH值、挥发性盐基氮含量和脂肪氧化值,并能抑制鱼肉色泽的变化。C1和C3在维持鱼肉品质上无显著差异。因此,含有肉桂油或者肉桂油和nisin的海藻酸钠薄膜可有效维持黑鱼的贮藏品质。

关键词：黑鱼肉桂油 nisin 海藻酸钠抗菌效果