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Respiratory Response of Dormant nectarine Vegetative Buds to High Temperature Stress

Journal of Integrative Agriculture 2013年第1期12卷 80-86页

作者： TAN Yue LI Ling LENG Chuan-yuan LI Dong-mei CHEN Xiu-de GAO Dong-sheng National Research Center for Apple Engineering and Technology/State Key Laboratory of Crop Biology/College of Horticulture Science and Engineering Shandong Agricultural University

High temperature stress （HT） is efficient in breaking endo-dormancy of perennial trees. The effects of HT （50°C） on the respiration of dormant nectarine （Prunus persica var. nectariana cv. Shuguang） vegetative buds were evaluated in the research. We found that bud respiration was transiently inhibited by HT and the pentose phosphate pathway （PPP） and the cytochrome C pathway （CYT） were significantly affected. On the substrate level, PPP was activated in the HT-treated buds compared with the control group. However, the activation did mot occur until hours after HT treatment. The tricarboxylic acid cycle （TCA） in both the HT-treated buds and in the control group proceeded at a low level most of the time compared with total respiration. On the electron transfer level, CYT was transiently inhibited by HT but became significantly active in the later stage. CYT operation in the control group exhibited an attenuation process. The alternative pathway （ALT） fluctuated both in the HT-treated samples and in the control. The results suggest that the temporary CYT inhibition and the following PPP activation may be involved in HT-induced bud dormancy release and budburst mechanisms.

关键词： high temperature stress dormancy release respiration nectarine vegetative buds

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RAPD MARKERS LINKED TO nectarine GENE AND FLESH COLOR GENE OF PEACH FRUIT

RAPD MARKERS LINKED TO NECTARINE GENE AND FLESH COLOR GENE O...

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International Symposium on Biotechnology Application in Horticultural Crops

作者： Zhang Kaichun Yang Yingjun Li Rongqi Lin Ke Jiang Quan Yin Shaping Guo Baolin Zhang XiaomingInstitute of forestry and pomologyBeijing academy of agriculture and forestry sciencesBeijing 100093 P. R. China

＜正＞ The population used was 69 hybrids derived from the intraspecific cross between peach variety "jingyu" [Primus persica （L.） Batsch.] and nectarine variety "flavor-top" （ Prunus persica Var. nectariana Maxim.） . RAPD fingerprinting technique was used to map nectarine gene （G） and flesh color gene （Y）, and 520 10 mer arbitrary primers were screened. Results showed that 158 primers were of polymorphism between the two parents - "jingyu" and "flavortop", Amplified polymorphic DNA fragment OPP20-2200 was linked to G gene with a distance of 5. 0 cM, and OPU03-850 was linked to Y gene with a distance of 9.6 cM.

关键词： RAPD marker nectarine flesh color

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Genome-wide identification of microRNAs that are responsive to virus/viroid infection in nectarine trees

Genome-wide identification of microRNAs that are responsive ...

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中国植物病理学会2021年学术年会

作者： YANG Lijuan LI Shifang ZHANG Zimeng LU Meiguang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences

Peach is one of the most widely grown fruit crops in China,and nectarine [Prunus persica（L.） Batsch ***（Suckow） ***.] is an important commercial variety of ***（miRNAs） are a class of non-coding RNAs that play important functions in posttranscriptional gene regulation in *** a number of miRNAs have been identified in peach trees,miRNAs in the different tissues of nectarine trees and in greenhouse-grown trees infected with various viruses or viroids have not been well *** this study,miRNAs and their target genes were identified and characterized by sequencing four small RNA libraries prepared from nectarine leaf and fruit tissues infected with different virus/viroid combinations using next-generation sequencing technology.A total of 116 known peach miRNAs belonging to 49 miRNA families were identified,and 303 putative novel miRNAs were *** of these miRNAs were found to be highly conserved,such as miR156,miR166,miR167,miR390,miR395,*** of the novel miRNAs were 24-nt in length and many of these miRNAs came from the MIR5271 and MIR7717 *** of potential targets of the differentially-expressed miRNAs showed that these miRNAs targeted 2,785 genes,including genes involved in transcriptional regulation and morphogenesis and biological processes and pathways such as metabolism,stress responses,and pathogen *** profiling revealed that 45 miRNAs belonging to 27 known miRNA families exhibited significant differential expression between leaf and fruit ***,the expression levels of these miRNAs were significantly affected in the two tissues despite the fact that the trees were infected by different virus/viroid combinations（infected by nectarine stem-pitting-associated virus and peach latent mosaic viroid or infected by nectarine stem-pitting-associated virus,peach latent mosaic viroid,apple chlorotic leaf spot virus,and asian prunus virus 2）;for example,miR167 a,miR172

关键词： nectarine microRNA leaf and fruit next-generation sequencing virus/viroid

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Effects of Short-term Freezing on Endodormancy Release in nectarine Bud

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Agricultural Science & Technology 2014年第3期15卷 367-369,431页

作者：魏国增赵海亮辽宁省果树科学研究所辽宁营口115009

[Objective] This study aimed to investigate the endodormancy release in nectarine bud treated by short-term freezing. [Method] Through short-term freezing at seven different temperatures for three different periods on bud, the livability, burst, ratio of free water to bound water and membrane permeability of ＇Shuguang＇ nec- tarine bud were studied. [Result] On November 10, compared with non-freezing treatment （CK）, the bud burst, ratio of free water to bound water and membrane permeability treated by freezing at -5 and -8 ℃ were almost the same as CK. But the rest freezing treatments advanced the date of endodormancy release, while the bud burst, ratio of free water to bound water and membrane permeability were high- er than CK. on November 20 and 30, the effects of the freezing treatment on en- dodormancy release were the same when the treatment on November 10, and the effect was better as the treatment was later. [Conclusion] The correlation of the rate of bud burst, ratio of free water to bound water, and membrane permeability of the different freezing treatments indicated that the change from bound water to free wa- ter and the increase of membrane permeability were probably the signal of endodor- mancy release.

关键词： Short-term freezing Endodormancy Bud nectarine

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A Role for GIBBERELLIN 2-OXIDASE6 and Gibberellins in Regulating Nectar Production

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Molecular Plant 2016年第5期9卷 753-756页

作者： Lisa B. Wiesen Ricci L. Bender Travis Paradis Alexie Larson M. Ann D.N. Perera Basil J. Nikolau Neil E. Olszewski Clay J. Carter Department of Plant Biology University of Minnesota Saint PaulMN 55108 USA Department of Biochemistry Biophysics & Molecular Biology Iowa State University Ames IA 50011 USA W.M.Keck Metabolomics Research Laboratory Iowa State University Ames IA 50011 USA Department of Plant Biology University of Minnesota Twin Cities 1445 Gortner Avenue 250 Biological Sciences Center Saint Paul MN 55108 USA

Dear Editor The regulation of nectar production is dependent on hormonal signaling. For example, Arabidopsis PIN6, which encodes an auxin efflux transporter family protein, was identified as a nectary-enriched gene whose expression level was positively correlated to total nectar production （Bender et al., 2013）. Furthermore, jasmonic acid （JA） levels peak in Brassica flowers just prior to anthesis and promote nectar secretion （Radhika et al., 2010）. While auxin and JA have been demonstrated to play roles in the regulation of nectar production,

关键词： GIBBERELLIN genetics nectarine RESEARCH

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Sugar and Acid Content and Their Genotypic Correlations of Unselected *** x *** Progenies as Affected by Hairness and Flesh Color

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Agricultural Sciences in China 2004年第2期3卷 136-142页

作者： WUBen-hong LIShao-hua QuilotBenedicte GenardMichel KervellaJocelyne InstituteofBotany ChineseAcademyofSciencesBeijing100093P.R.China INRA UnitePlantesetSystemesdecultureHorticolesDomaineStPaulSiteAgroparcAvignon84914Cedex9France INRA UnitedeGenetiqueetd＇AmeliorationdesFruitsetLegumesDomaineStPaulSiteAgroparcAvignon84914Cedex9France

Fruit sugar and acid content were determined in a group of hybrids from one family,including 22 peach and 85 nectarine genotypes, or 75 white-fleshed and 32 yellow-fleshedgenotypes. Sucrose accounted for about 65% of the total sugar, and malate for about 50%of the total acid. Mean sucrose, fructose, sorbitol, total sugar, soluble solid andquinate content were significantly higher in nectarines than in peaches, while nosignificant difference was found in mean malate, citrate and total acid content betweennectarines and peaches. Shikimate content was very low and ranged from 0.011-0.046 meq100 g-1 FW in all genotypes studied. Flesh color did not affect sugar and acid *** and fructose had a close positive correlation (r =0.63***). Malate had positivecorrelations with sucrose, sorbitol, total sugar and soluble solid content. The correlationbetween citrate and sugars were not significant. Glucose, fructose, sorbitol and malatecontent seemed to be higher in late-maturing genotypes. Sucrose, total sugar and totalacid content had a parabolic relationship with developmental period (from full bloomingdate to maturity date).

关键词： Acid Flesh color nectarine Peach Sugar

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