检索结果-南通市图书馆

Postnatal development of nadph-diaphorase expression in the visual cortex of the golden hamster

Neural Regeneration Research 2011年第28期6卷 2165-2170页

作者： Ying Xu Yuemei Xiao Yuncheng Diao Kwok-Fai So Joint laboratory for Brain Function and Health Jinan University and the University of Hong Kong Jinan University Medical School Guangzhou 510632 Guangdong Province China Laboratory of Visual Information Processing Institute of Biophysics Academia Sinica Beijing 100101 China Department of Anatomy the University of Hong Kong Pokfulam Hong Kong China

Nitric oxide is an important neuromodulator in the brain and is involved in the development of visual system. But it is not clear how nitric oxide and nitric oxide synthase （NOS） are involved in the developing visual cortex of rodents. Thus we examined the expression of NOS activity in the postnatal developing visual cortex of the golden hamster by using histochemical technique for nadph-diaphorase （nadph-d）. A heavily stained nadph-d band was observed in the neuropil of the visual cortex. This nadph-d band initially appeared in the cortical plate from the day of birth （P0） to postnatal day 4 （P4）. From P7 to P21, this band was confined to area 17 and migrated to the deeper layers Ill IV and V VI before it eventually disappeared at P28. Such developmental trends of the band correlated well with the process of formation and establishment of the geniculo-cortical projection patterns. Thus, the areal specific development of the band suggests that NOS is closely related to the cortical differentiation and synaptic formation of the primary visual cortex. On the other hand, monocular eye enucleation on P1 could not alter the appearance of this nadph-d positive band, indicating a non-activity dependant role of NOS. In addition, differences in the laminar distributions and developmental sequence between the heavily and lightly stained nadph-d positive neurons during development suggest that they play different roles in the development.

关键词： nadph-diaphorase nitric oxide synthase postnatal development visual cortex area 17 golden hamster neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Method for Detecting nadph-Cytochrome P450 Reductase in Liver Microsomal Fractions by Using Native Polyacrylamide Gel Electrophoresis and nadph-diaphorase Staining

引用

American Journal of Analytical Chemistry 2013年第6期4卷 301-305页

作者： Hirokazu Yokoyama Yukishige Okamura Toshifumi Hibi Department of Internal Medicine School of Medicine Keio University Shinanomachi Shinjukuku Tokyo Japan

By combining native polyacrylamide gel electrophoresis (PAGE) and nicotinamide adenine dinucleotide phosphate (nadph)-diaphorase staining, a simple method for detecting nadph-cytochrome P450 reductase in tissue samples was established. When rat liver microsomal fractions were examined by this method, several bands with different mobility were visualized. Western blot analysis indicated that the band which appeared in the most anodal position among them represented nadph-cytochrome P450 reductase. SDS-PAGE/Western blot analysis revealed that the molecular weight of the protein forming the band was around 80 kDa, which was identical to that of rat nadph-cytochrome P450 reductase. The intensity level of nadph-diaphorase staining assigned to this enzyme estimated by this method increased four times in microsomal fractions prepared from rat fed ethanol chronically compared to that from controls. When a dilution series of a rat liver microsomal fraction was examined by this method and SDS-PAGE/Western blot analysis, their staining intensities representing this enzyme were significantly correlated with each other. Using the naive PAGE/nadph-diaphorase staining method, nadph-cytochrome P450 reductase is detected in rat liver microsomes. This method is beneficial because compared with the conventional SDS-PAGE/Western blot analysis, the quantification of nadph-cytochrome P450 reductase in tissue samples is allowed to be more easily done.

关键词： nadph-Cytochrome p450 Reductase Native PAGE nadph-diaphorase Staining Chronic Ethanol Consumption

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Features of adult neurogenesis and neurochemical signaling in the Cherry salmon Oncorhynchus masou brain

引用

Neural Regeneration Research 2013年第1期8卷 13-23页

作者： Evgeniya V. Pushchina Dmitry K. Obukhov Anatoly A. Varaksin Laboratory of Cytophysiology Institute of Marine Biology Far East Branch Russian Academy of Sciences St. Petersburg State University

We investigated the distribution of gamma aminobutyric acid, tyrosine hydroxylase and nitric oxide-producing elements in a cherry salmon Oncorhynchus masou brain at various stages of postnatal ontogenesis by immunohistochemical staining and histochemical staining. The periventricular region cells exhibited the morphology of neurons and glia including radial glia-like cells and contained several neurochemical substances. Heterogeneous populations of tyrosine hydroxylase-, gamma aminobutyric acid-immunoreactive, as well as nicotinamide adenine dinucleotide phosphate diaphorase-positive cells were observed in proliferating cell nuclear antigen-immunoreactive proliferative zones in periventricular area of diencephalon, central grey layer of dorsomedial tegmentum, medulla and spinal cord. Immunolocalization of Pax6 in the cherry salmon brain revealed a neuromeric construction of the brain at various stages of postnatal ontogenesis, and this was confirmed by tyrosine hydroxylase and gamma aminobutyric acid labeling.

关键词： neural regeneration neurogenesis teleostei adult neurogenesis neurotransmitter signaling migration tyrosine hydroxylase gamma aminobutyric acid development Pax6 nadph-diaphorase proliferation grant-supported paper neuroregeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Interaction of Atoms with Grain Surfaces in Steel: Periodic Dependence of Binding Energy on Atomic Number and Influence on Wear Resistance

引用

Engineering（科研） 2012年第10期4卷 76-79页

作者： E. V. Pushchina D. K. Obukhov A. V. Zhirmunski Institute of Marine Biology Far East Division Russian Academy of Sciences ul. Pal'chevskii 17 Vladivostok 690059 Russia ru St. Petersburg State University Universitetskaya Naberezhnaya 7/9 St. Petersburg 199034 Russia

The data of our investigations contribute to understanding of cellular mechanisms of the teleost fishes CNS forming in postembryonic development. The revealed peculiarities of structural and neurochemical organization and description of basic histogenetic processes (proliferation, migration and neuronal cell differentiation) during the brain forming in fish, which have signs of fetal organization, widen the existing knowledge about histogenesis of these structures in postembryonic development. It seems conceivable, that during postembrional development in teleost fishes some neurotransmitters and gaseous mediators (NO and H2S) act as factors, which initiate and regulate the cellular and the tissues processes of genetic program during the brain development. Materials of this investigation define a new experimental model for studying of postembrional neurogenesis processes.

关键词： Teleostei Postnatal Neurogenesis Proliferative Cell Nuclear Antigen (PCNA) Neurotransmitter Signaling Migration Tyrosine Hydroxylase GABA Development Pax6 nadph-diaphorase Nitric Oxide Hydrogen Sulfide Proliferation.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The　dynamic　distribution　of　nitric　oxide　synthase　in　the　small　intestine　of　mice　with　intestinal　radiation　sickness

引用

Journal of Medical Colleges of PLA(China) 1996年第2期11卷 147-150,154页

作者：魏丽春郭鹞

in order to understand the dynamic change of nitric oxide (NO) and its pathological significance during intestinal radiation sickness, we gave mice 60Co γ-ray irradiation and sacrificed them after different periods of *** dynamic distribution of nitric oxide synthase (NOS) in the small intestines (duodenum, jejunum and ileum) was studied by using nadph-diaphorase histochemistry. The results showed that in the animal groups that survived 48 h and 72 h after irradiation (the 48 h and 72 h groups), the number of NOS-positive neuronal bodies and the density of NOS-positive fibers increased obviously in the myenteric plexus and deep muscular plexus as compared with the control group. The neuronal bodies and fibers were stained intensely. The NOS-positive reaction product in the glandular cavities of the intestines and on the mucosa also increased. The results suggest that the activation of NOS or increase of NO production might be one of the main causes of intestinal and vascular dilatation during intestinal radiation sickness.

关键词： intestinal radiation sickness nitric oxide synthase nadph-diaphorase histochemistry small intestine mice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The histochemical localization of nitric oxide synthase in mouse intestines

引用

Journal of Medical Colleges of PLA(China) 1995年第2期10卷 109-112,117页

作者：魏丽春郭鹞

In the present study,the localization of nitric oxide synthase(NOS) in the mouse intestines(duodenum,jejunum,ileum and proximal segment of large intestine) was observed using nadph-diaphorase(ND) histochemical *** res... 详细信息

关键词： nitric oxide synthase nadph-diaphorase histochemistry intestines mice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

丙烯酰胺和2,5己二酮对大鼠神经毒作用的研究

丙烯酰胺和2,5己二酮对大鼠神经毒作用的研究

引用

作者：侯维锦州医科大学

学位级别：硕士

目的在丙烯酰胺（acrylamide,AA）和2,5己二酮（2,5-hexanedione,2,5-HD）的暴露下,检测年轻大鼠感觉运动功能障碍,并探究在毒素作用下大鼠延髓及脊髓后索尼克酰胺腺嘌呤二核苷酸磷酸-黄递酶（Nicotinamide adenine dinucleotide phosph... 详细信息

目的在丙烯酰胺（acrylamide,AA）和2,5己二酮（2,5-hexanedione,2,5-HD）的暴露下,检测年轻大鼠感觉运动功能障碍,并探究在毒素作用下大鼠延髓及脊髓后索尼克酰胺腺嘌呤二核苷酸磷酸-黄递酶（Nicotinamide adenine dinucleotide phosphate diaphorase,nadph-d）阳性反应特征和毒素对神经组织细胞及神经递质的影响。方法正常2月龄雄性SD大鼠60只随机分为对照组、AA组和2,5-HD组,每组20只,AA组和2,5-HD组分别给予25 mg/kg AA、300 mg/kg 2,5-HD,对照组给予等体积的生理盐水,均采用腹腔注射的给药方式,每周周1至周5给药,连续给药9周。各组动物每周周日记录体重变化;各组大鼠在给药期评估大鼠步态并检测50℃热水浴甩尾撤退时间。给药9周后行灌注固定,在冰上迅速取出大脑和脊髓浸泡于梯度蔗糖多聚甲醛溶液中脱水固定。各组动物延髓薄束核及脊髓进行nadph-d组织化学染色,延髓薄束核行胶质纤维酸性蛋白（glial fibrillary acidic protein,GFAP）、神经元核心抗原（Neuronal nuclei,Neu N）小胶质细胞/巨噬细胞特异性蛋白、神经肽Y（neuropeptide Y,NPY）、（ionized calcium binding adapter molecule 1,Iba1）、微管相关蛋白2（microtubule-associated protein 2,MAP2）、降钙素基因相关肽（calcitonin gene related peptide,CGRP）等免疫组织化学或免疫荧光显色反应,观察毒素对薄束核神经组织细胞及神经递质表达的影响。结果与对照组相比,AA组与2,5-HD组大鼠体重明显降低（P＜0.01）。步态评估中,AA组和2,5-HD组大鼠从4周开始均出现明显步态评分异常（P＜0.01）。在50℃热水浴甩尾实验中,与对照组相比,AA组在给药4周后,热水浴甩尾撤退时间缩短（P＜0.05）,8周后撤退时间与对照组相比无明显差别;而2,5-HD组甩尾撤退时加与对照组相比未见明显差别。对于nadph-d组织化学显色反应,AA组大鼠延髓薄束核区未出现明显的年龄相关性nadph-d异常阳性体（aging-related nadph-diaphorase positive body,ANB）,但薄束核区nadph-d阳性细胞明显增多（P＜0.05）增多,免疫组织化学显示薄束核GFAP、Iba1、NPY表达增加,MAP2和Neu N表达降低。2,5-HD组薄束核区和颈胸段后索薄束出现了比较明显的ANB,薄束核免疫组织化学反应显示薄束核GFAP表达明显增加（P＜0.01）,MAP2和Neu N表达降低。结论1.毒素AA和2,5-HD导致的脊髓和延髓退行性变不同于自然衰老的病理变化,ANB作为自然状态下神经衰老的标志有其特殊性。2.毒素AA早期可造成大鼠感觉异常,致使痛觉过敏,可能与NO和NPY有关,而晚期没有痛觉过敏反应,可能与感觉性神经元缺失有关。3.毒素AA和2,5-HD均可对神经造成炎性损伤。

关键词：薄束核 nadph-diaphorase 丙烯酰胺 2,5-己二酮

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：